《中国康复理论与实践》

中国康复理论与实践 >

2019 , Vol. 25 >Issue 10: 1133 - 1139

DOI: https://doi.org/10.3969/j.issn.1006-9771.2019.10.004

专题 脊髓损伤康复

电针对创伤性脊髓损伤大鼠下肢骨骼肌萎缩相关蛋白表达的影响

  • 范蕊 ,
  • 吴宗辉 ,
  • 陈晓琳 ,
  • 邹佐强 ,
  • 龙在云 ,
  • 姚兰 ,
  • 李斌
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  • 1.西南大学医院，重庆市400715;
    2.西南大学运动康复研究所，重庆市400715;
    3.陆军军医大学大坪医院野战外科研究所第三研究室，创伤与烧伤国家重点实验室，重庆市 400042
范蕊(1980-)，女，汉族，河南平顶山市人，硕士，主治医师，主要研究方向：物理因子治疗及运动损伤康复。

网络出版日期: 2019-10-30

基金资助

1.重庆市科委社会事业与民生保障科技创新项目(No. CSTC2016SHMZX130016)；2.中央高校基本科研基金项目(No. XDJK2017C090)；3.西南大学医院科研资金资助项目(No. xdyykyjj2019xm04)

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Effects of Electroacupuncture on Skeletal Muscle Atrophy-associated Protein in Hind Limbs of Traumatic Spinal Cord Injury Rats

  • FAN Rui ,
  • WU Zong-hui ,
  • CHEN Xiao-lin ,
  • ZOU Zuo-qiang ,
  • LONG Zai-yun ,
  • YAO Lan ,
  • LI Bin
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  • 1.Southwest University Hospital, Chongqing 400715, China;
    2.Institute of Sports Rehabilitation, Southwest University, Chongqing 400715, China;
    3.State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University, Chongqing 400042, China

Online published: 2019-10-30

Supported by

Supported by Scientific and Technological Innovation Project of Social Career and People's Livelihood Guarantee of Chongqing Science and Technology Commission (No. CSTC2016SHMZX130016), The Fundamental Research Funds for the Central Universities (No. XDJK2017C090) and Southwest University Hospital Scientific Research Project (No. xdyykyjj2019xm04)

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摘要

目的 观察电针对创伤性脊髓损伤(TSCI)大鼠腓肠肌中肌肉生长抑制素(MSTN)、肌肉特异性环指蛋白1 (MuRF1/Trim63)、肌肉萎缩盒F蛋白32 (Atrogin-1/Fbxo32)、成肌分化抗原(Myod)和肌细胞生成素(Myog) mRNA表达的影响，探讨电针延缓TSCI大鼠下肢萎缩的可能机制。 方法 将雌性Sprague-Dawley大鼠45只随机分为假手术组(n = 12)和造模组(n = 33)。采用 Allen法制备大鼠TSCI模型。将造模组存活大鼠(n = 24)随机分为模型组(n = 12)和电针组(n = 12)。术后1 d，电针组电针大椎、命门和双侧足三里穴，共28 d。术前，术后3 d、7 d、14 d、21 d、28 d比较各组BBB评分；术前和术后28 d，比较各组大鼠体质量；术后28 d，比较各组腓肠肌湿重比，HE染色比较腓肠肌纤维截面积和直径，实时定量聚合酶链反应(PCR)比较各组腓肠肌中MSTN、Trim63、Fbxo32、Myod和Myog mRNA的相对表达量。 结果 术后3 d、7 d、14 d、21 d和28 d，模型组BBB评分明显低于假手术组(P < 0.01)，术后7 d、14 d、21 d和28 d，电针组BBB评分明显高于模型组(P < 0.01)。术后28 d，与假手术组比较，模型组体质量、左右侧肌湿重比、肌纤维横截面积和直径均减小(P < 0.01)，MSTN、Trim63、Fbxo32、Myod和Myog mRNA的相对表达量升高(P < 0.05)；与模型组比较，电针组左右侧肌湿重比、肌纤维横截面积和直径均增加(P < 0.05)，MSTN、Trim63和Fbxo32 mRNA相对表达量降低(P < 0.05)，Myod和Myog mRNA相对表达量升高(P < 0.05)。 结论 电针干预大鼠大椎、命门和双侧足三里穴可能通过下调MSTN、Trim63、Fbxo32 mRNA的表达，并上调Myod和Myog mRNA的表达，促进肌卫星细胞的成肌分化，延缓肌蛋白降解，从而减轻TSCI大鼠下肢腓肠肌萎缩。

关键词： 创伤性脊髓损伤; 肌萎缩; 电针; 肌肉生长抑制素; 肌卫星细胞; 大鼠

本文引用格式

范蕊 , 吴宗辉 , 陈晓琳 , 邹佐强 , 龙在云 , 姚兰 , 李斌 . 电针对创伤性脊髓损伤大鼠下肢骨骼肌萎缩相关蛋白表达的影响[J]. 中国康复理论与实践, 2019 , 25(10) : 1133 -1139 . DOI: 10.3969/j.issn.1006-9771.2019.10.004

Abstract

Objective To explore the effects and mechanism of electroacupuncture (EA) on expression of myostatin (MSTN), muscle-specific ring finger protein 1 (MuRF1/Trim63), F-box only protein 32 (Atrogin-1/ Fbxo32), myogenic differentiation antigen (Myod) and myogenin (Myog) in traumatic spinal cord injury (TSCI) rats. Methods A total of 45 adult female Sprague-Dawley rats were randomly divided into sham operation group (n = 12) and operation group (n = 33). The TSCI model was established with the modified Allen's method. After modeling, there were 24 survival rats and they were randomly divided into model group (n = 12) and EA group (n = 12). EA group was electroacupunctured at Dazhui (DU 14), Mingmen (DU 4) and bilateral Zusanli (ST 36) for 10 minutes, once a day, six times a week for 28 days. Basso-Beattie-Bresnahan (BBB) score was tested before modeling, and three days, seven days, 14 days, 21 days and 28 days after modeling. The rats were measured their body mass before and 28 days after modeling. The ratio of gastrocnemius wet mass was calculated; the cross-sectional area (CSA) and fiber diameter were measured by HE staining; the expression of MSTN, Trim63, Fbxo32, Myod and Myog mRNA were tested with real-time quantitative polymerase chain reaction (qPCR). Results Three days, seven days, 14 days, 21 days, and 28 days after modeling, the score of BBB was lower in the model group than in the sham operation group (P < 0.01); seven days, 14 days, 21 days, and 28 days after modeling, the score of BBB was higher in EA group than in the model group (P < 0.01). Compared with the sham operation group, the mass of rats, the gastrocnemius wet mass, the CSA and the diameter of the muscle fiber were smaller in the model group (P < 0.05), while the expression of MSTN, Trim63, Fbxo32, Myod and Myog mRNA were higher (P < 0.05). Compared with the model group, the mass of rats, the gastrocnemius wet mass, the CSA, the expression of Myod and Myog mRNA were higher (P < 0.05) in EA group, while the expression of MSTN, Trim63 and Fbxo32 mRNA were lower (P < 0.05). Conclusion EA might delay the gastrocnemius atrophy in TSCI rats by down-regulating the expression of MSTN, Trim63, Fbxo32 mRNA and up-regulating the expression of Myod and Myog mRNA via controlling the differentiation of the muscle satellite cells and the degradation of protein in skeletal muscle cells.

Key words： traumatic spinal cord injury; muscle atrophy; electroacupuncture; myostatin; muscle satellite cells; rats

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