《中国康复理论与实践》

中国康复理论与实践 >

2020 , Vol. 26 >Issue 3: 319 - 324

DOI: https://doi.org/10.3969/j.issn.1006-9771.2020.03.011

电针对血管性痴呆大鼠脑白质纤维和学习记忆功能的效果

  • 张冰雪 ,
  • 杨敏光 ,
  • 李建鸿 ,
  • 陶静 ,
  • 梁胜祥 ,
  • 柳维林 ,
  • 陈立典
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  • 1.福建中医药大学康复医学院,福建福州市 350122
    2.福建中医药大学康复医疗技术国家地方联合工程研究中心,福建福州市 350122
    3.福建省康复技术协同中心,福建福州市 350122
张冰雪(1995-),女,汉族,贵州六盘水市人,硕士研究生,主要研究方向：神经康复与认知科学。|陈立典(1963-),男,福建政和县人,博士,教授,主任医师,博士生导师,主要研究方向：神经康复及认知科学。

收稿日期: 2019-09-27

  修回日期: 2020-01-20

  网络出版日期: 2020-04-01

基金资助

国家自然科学基金项目(81774424)

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Effects of Electroacupuncture on White Matter Fibers and Learning-memory Function in Rats with Vascular Dementia

  • Bing-xue ZHANG ,
  • Min-guang YANG ,
  • Jian-hong LI ,
  • Jing TAO ,
  • Sheng-xiang LIANG ,
  • Wei-lin LIU ,
  • Li-dian CHEN
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  • 1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
    2. National & Local Joint Engineering Research Center of Rehabilitation Medical Technology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
    3. Fujian Collaborative Innovation Center for Rehabilitation Technology, Fuzhou, Fujian 350122, China

Received date: 2019-09-27

  Revised date: 2020-01-20

  Online published: 2020-04-01

Supported by

National Natural Science Foundation of China(81774424)

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摘要

目的 观察电针百会、神庭对血管性痴呆(VD)大鼠脑白质纤维和学习记忆能力的影响。 方法 Sprague-Dawley大鼠32只分离双侧颈总动脉,假手术组(n = 8)不结扎,其余造模成功后随机分为模型组(n = 8)、非穴组(n = 8)和电针组(n = 8)。电针组电针百会和神庭穴,非穴组刺激腋下非经非穴点,每天1次,共28 d。干预前后采用新物体识别试验进行评定,弥散张量成像观察大鼠脑白质纤维。 结果 干预前,与假手术组相比,模型组、非穴组和电针组新物体偏爱系数降低(P< 0.05),后三组间无显著性差异(P> 0.05);干预后,电针组新物体偏爱系数较模型组增高(P < 0.05)。干预前,与假手术组相比,模型组、非穴组和电针组胼胝体、扣带回、海马白质纤维各向异性分数(FA)降低;干预后,电针组海马、扣带回、胼胝体、外囊白质纤维FA较模型组增加。 结论 电针百会、神庭能改善VD大鼠记忆功能,可能与前额叶皮质、海马等脑区白质纤维修复有关。

关键词： 血管性痴呆; 电针; 白质纤维; 弥散张量成像; 学习记忆; 大鼠

本文引用格式

张冰雪 , 杨敏光 , 李建鸿 , 陶静 , 梁胜祥 , 柳维林 , 陈立典 . 电针对血管性痴呆大鼠脑白质纤维和学习记忆功能的效果[J]. 中国康复理论与实践, 2020 , 26(3) : 319 -324 . DOI: 10.3969/j.issn.1006-9771.2020.03.011

Abstract

Objective To observe the effect of electroacupuncture at Baihui (DU20) and Shenting (DU24) acupoints on white matter fiber and learning-memory function in rats with vascular dementia (VD). Methods Sprague-Dawley rats were randomly divided into operation group and sham group (n = 8). The operation group accepted two-vessel occlusion, while the sham group only separated the bilateral common carotid arteries without ligation. The rats modeled successfully were randomly divided into model group (n = 8), non-acupoint group (n = 8) and electroacupuncture group (n = 8). The electroacupuncture group accepted electroacupuncture at Baihui and Shenting, and the non-acupoint group accepted electroacupuncture at axillary non-acupoint, once a day for 28 days. All the rats were tested with object recognition test before and after intervention, while the white matter fibers were observed with Diffusion Tensor Imaging. Results Compared with the sham group, the preference coefficients of the model group, the non-acupoint group and the electroacupuncture group decreased before intervention (P< 0.05), and there was no significant difference among the later three groups (P> 0.05). The preference coefficients increased in the electroacupuncture group compared with the model group after intervention (P< 0.05). The fractional anisotropy (FA) of corpus callosum, cingulate gyrus and hippocampus decreased in the model group, the non-acupoint group and the electroacupuncture group compared with the sham group before intervention. FA of hippocampus, cingulate gyrus, corpus callosum and external capsule increased in the electroacupuncture group compared with the model group after intervention. Conclusion Electroacupuncture at Baihui and Shenting can improve learning-memory function of VD rats, which may be related with repairment of white matter fibers in prefrontal cortex, hippocampus and others.

Key words： vascular dementia; electroacupuncture; white matter fiber; diffusion tensor imaging; learning and memory; rats

参考文献

[1] Rizzi L, Rosset I, Roriz-Cruz M. Global epidemiology of dementia: Alzheimer's and vascular types[J]. Biomed Res Int, 2014, 2014:908915.
[2] Zhang Y D, Xu Y, Nie H W, et al. Prevalence of dementia and major dementia subtypes in the Chinese populations: a meta-analysis of dementia prevalence surveys, 1980–2010[J]. J Clin Neurosci, 2012, 19(10):1333-1337.
[3] Gorelick P B, Scuteri A, Black S E, et al. Vascular contributions to cognitive impairment and dementia: a statement for healthcare professionals from the American Heart Association/American Stroke Association[J]. Stroke, 2011, 42(9):2672-2713.
[4] Sebastien V, Lopes R, Lenfant P, et al. Cerebral hypoperfusion and hypometabolism detected by arterial spin labeling MRI and FDG-PET in early-onset Alzheimer's disease[J]. J Neuroimaging, 2016, 26(2):207-212.
[5] Cao X Y, Yao Y, Li T, et al. The impact of cognitive training on cerebral white matter in community-dwelling elderly: one-year prospective longitudinal diffusion tensor imaging study[J]. Sci Rep, 2016, 6(1):33212.
[6] Lopez-Oloriz J, Lopez-Cancio E, Arenillas J F, et al. Diffusion tensor imaging, intracranial vascular resistance and cognition in middle-aged asymptomatic subjects[J]. Cerebrovasc Dis, 2014, 38(1):24-30.
[7] Wu Y F, Wu W B, Liu Q P, et al. Presence of lacunar infarctions is associated with the spatial navigation impairment in patients with mild cognitive impairment: a DTI study[J]. Oncotarget, 2016, 7(48):78310-78319.
[8] Lin L, Xue Y J, Duan Q, et al. Microstructural white matter abnormalities and cognitive dysfunction in subcortical ischemic vascular disease: an atlas-based diffusion tensor analysis study[J]. J Mol Neurosci, 2015, 56(2):363-370.
[9] Tuladhar A M, van Norden A G W, de Laat K F, et al. White matter integrity in small vessel disease is related to cognition[J]. Neuroimage Clin, 2015, 7:518-524.
[10] Huang F, Kuang W C, Zhou F X, et al. Therapeutic efficacy observation on acupuncture treatment for vascular cognitive disorder following a cerebral infarction[J]. J Acup Tuina Sci, 2012, 10(1):29-33.
[11] 石景洋, 张彦丽, 唐学敏. 电针对血管性痴呆患者认知功能及行为能力的影响[J]. 中医杂志, 2012, 53(8):697-699.
[12] 孟培燕, 孙国杰, 毛娟娟. 电针对血管性痴呆模型大鼠学习记忆能力的影响[J]. 针灸推拿医学, 2012, 10(2):67-71.
[13] 梁慧英, 林阳阳, 燕铁斌. 电针对血管性痴呆模型大鼠记忆能力的影响[J]. 中国康复医学杂志, 2015, 30(6):527-531.
[14] Kim Y J, Kwon H K, Lee J M, et al. White matter microstructural changes in pure Alzheimer's disease and subcortical vascular dementia[J]. Eur J Neurol, 2015, 22(4):709-716.
[15] Correia S, Lee S Y, Voorn T, et al. Quantitative tractography metrics of white matter integrity in diffusion-tensor MRI[J]. Neuroimage, 2008, 42(2):568-581.
[16] Wu Z, Hu J, Du F, et al. Long-term changes of diffusion tensor imaging and behavioural status after acupuncture treatment in rats with transient focal cerebral ischaemia[J]. Acupunct Med, 2012, 30(4):331-338.
[17] 李晓洁, 俞坤强, 赵从快. 电针神庭、百会对大脑中动脉闭塞大鼠学习记忆的影响及机制研究[J]. 中国康复医学杂志, 2017, 32(8):869-873.
[18] 林浴坤, 陶静, 陈斌, 等. 电针对局灶性脑缺血再灌注损伤大鼠学习记忆功能的影响[J]. 中国康复理论与实践, 2014, 20(9):831-834.
[19] 赵从快, 柳维林, 林如辉, 等. 电针百会、神庭穴对脑缺血再灌注大鼠学习记忆影响的小动物磁共振波谱研究[J]. 康复学报, 2016, 26(2):21-27.
[20] Huang S L, Chang C W, Lee Y H, et al. Protective effect of low-intensity pulsed ultrasound on memory impairment and brain damage in a rat model of vascular dementia[J]. Radiology, 2017, 282(1):113-122.
[21] 方剑乔, 郭义. 实验针灸学[M]. 北京: 中国中医药出版社, 2012.
[22] Xie G, Song C, Lin X, et al. Electroacupuncture regulates hippocampal synaptic plasticity via inhibiting Janus-activated kinase 2/signal transducer and activator of transcription 3 signaling in cerebral ischemic rats[J]. J Stroke Cerebrovasc Dis, 2019, 28(3):792-799.
[23] Gaskin S, Tardif M, Cole E, et al. Object familiarization and novel-object preference in rats[J]. Behav Processes, 2010, 83(1):61-71.
[24] Nie B B, Chen K W, Zhao S J, et al. A rat brain MRI template with digital stereotaxic atlas of fine anatomical delineations in Paxinos space and its automated application in voxel-wise analysis[J]. Hum Brain Mapp, 2013, 34(6):1306-1318.
[25] Liang S X, Wu S, Huang Q, et al. Rat brain digital stereotaxic white matter atlas with fine tract delineation in Paxinos space and its automated applications in DTI data analysis[J]. Magn Reson Imaging, 2017, 43:122-128
[26] 杨辰华, 王永炎. 血管性痴呆的中医病机及辨治思路[J]. 中医研究, 2005, 18(5):6-7.
[27] 高强, 张斐姝, 张丹丹. 血管性痴呆中医病机及人参皂苷益智机制研究进展[J]. 中华中医药杂志, 2018, 33(12):238-240.
[28] 武峻艳, 王杰, 张俊龙. 从督脉论治不同脑老化疾病异病同治的理论基础——以阿尔茨海默病和帕金森病为例[J]. 中国针灸, 2015, 35(5):489-492.
[29] 贾玉洁, 高靓, 张雪竹. 近5年针刺治疗血管性痴呆机制研究进展[J]. 江西中医药, 2017, 48(8):71-73.
[30] 蒋灵鸽, 张华伟, 张拯, 等. 双强度、频度电针对血管性痴呆大鼠海马CA1区β淀粉样蛋白1-40、精氨酸加压素及学习记忆的影响[J]. 针刺研究, 2017, 42(1):20-24.
[31] 马莉, 顾兰馨, 王诗陶, 等. 近10年针刺治疗血管性痴呆临床研究进展[J]. 针灸临床杂志, 2018, 34(8):86-89.
[32] 邵瑛, 赖新生, 关楚威, 等. “通督调神固本”电针法对高血压-高血脂复合血管性痴呆模型大鼠学习记忆干预的初步研究[J]. 针刺研究, 2009, 34(6):368-375.
[33] 江一静, 范文曦, 林凌, 等. 电针百会穴、神庭穴对血管性认知功能障碍的临床研究[J]. 世界中医药, 2019, 14(2):473-476.
[34] 李静, 白玉, 张鹏, 等. 脱氢表雄酮对血管性痴呆小鼠学习和记忆能力的改善作用[J]. 中国病理生理杂志, 2018, 34(10):1905-1909.
[35] Debette S, Markus H S. The clinical importance of white matter hyperintensities on brain magnetic resonance imaging: systematic review and meta-analysis[J]. BMJ, 2010, 341(1):c3666.
[36] Price C C, Mitchell S M, Brumback B, et al. MRI-leukoaraiosis thresholds and the phenotypic expression of dementia[J]. Neurology, 2012, 79(8):734-740.
[37] Lundeberg T, Lund I, Naslund J. Acupuncture–self-appraisal and the reward system[J]. Acupunct Med, 2007, 25(3):87-99.
[38] 杨烁慧, 陆方, 潘卫东, 等. 针刺改善急性脑缺血后脑白质损伤的DTI研究[J]. 中国中西医结合影像学杂志, 2013, 11(5):463-465.
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