《中国康复理论与实践》

《中国康复理论与实践》

• 础基研究 • 上一篇    下一篇

运动时不同吸氧浓度对慢性阻塞性肺疾病大鼠活性氧类物质产生的影响①

刘衡1，温宝伶2，王晓龙2   

  1. 1. 重庆大学体育学院，重庆市400030；2. 重庆医科大学第二临床学院急救部，重庆市400010。
  • 出版日期:2016-07-25 发布日期:2016-09-22

Effect of Oxygen Concentration during Exercise on Reactive Oxygen Species Production in Chronic Obstructive Pulmonary Disease Rats

LIU Heng1, WEN Bao-ling2, WANG Xiao-long2   

  1. 1. Sports Institute of Chongqing Universty, Chongqing 400030, China; 2. Emergency Department, the 2nd Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
  • Published:2016-07-25 Online:2016-09-22

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摘要： 目的探讨运动时吸氧浓度差异性对慢性阻塞性肺疾病(COPD)活性氧类物质(ROS)产生的影响及可能机制。方法Wistar 大鼠80 只建立COPD模型，分为低氧(LO, n=20)、常氧(NO, n=20)、吸氧(IO, n=20)运动组及常氧非运动对照(C, n=20)组。前3 组每天分别置氧体积浓度13.6%、21%、25%环境中跑台运动1 h，C 组于氧浓度21%下置静止跑台上1 h。各组运动后1 周(n=10)、4 周(n=10)，流式细胞仪检测血中性粒细胞凋亡率及ROS 含量，免疫组化检测肺组织还原型谷胱甘肽(GSH)表达，Westernblotting 检测骨骼肌细胞色素C氧化酶亚基Ⅳ(COXⅣ)蛋白表达。结果运动1 周时，LO组、NO组ROS较C组升高(P<0.05)，中性粒细胞凋亡率下降(P<0.05)，GSH及COXⅣ与C组无显著性差异(P>0.05)。运动4 周时，LO组ROS含量较C组升高(P<0.05)，NO组和IO组ROS较C组减低(P<0.05)；NO组和IO组GSH、COXⅣ含量较C组增加(P<0.05)，LO组较C组减少(P<0.05)。结论非低氧状态COPD患者进行长期康复运动，可能通过促进中性粒细胞凋亡、增强抗氧化能力等抑制体内ROS产生。

关键词: 慢性阻塞性肺疾病, 运动, 氧浓度, 吸氧, 活性氧类物质, 大鼠

Abstract: Objective To investigate the effect of various concentration of inhaled oxygen during exercise on reactive oxygen species (ROS) production in chronic obstructive pulmonary disease (COPD) rats and the possible mechanism. Methods Eighty COPD Wistar rats were divided into low oxygen (LO, n=20), normal oxygen (NO, n=20) and inhaled oxygen (IO, n=20) exercising groups, which ran on treadmill in the conditions of 13.6%, 21%, 25% oxygen, respectively, and non-exercising normal oxygen group (C, n=20), which stood on still treadmill in the condition of 21% oxygen. Their apoptosis percentage of neutrophils and ROS content were measured with flow cytometry, glutathione (GSH) in lung with immunohistochemistry and cytochrome C oxidase IV (COXIV) in skeletal muscle with Western blotting, as one and four weeks of exercising. Results For one week of exercise, the ROS was more in LO and NO groups than in C group (P<0.05), while the neutrophils apoptosis percentage was less (P<0.05); and there was no difference among C, LO, NO, IO groups in expression of GSH and COXIV (P>0.05). For four weeks of exercise, the ROS was more in LO group but less in NO and IO groups than in C group (P< 0.05); while the expression of GSH and COXIV increased in NO and IO groups compared with those in C group (P<0.05). Conclusion A long term exercise in non-hypoxic state may inhibit ROS production for COPD patients, by promoting neutrophils apoptosis and antioxidant expression.

Key words: chronic obstructive pulmonary disease, exercise, oxygen concentration, inhaled oxygen, reactive oxygen species, rats