《中国康复理论与实践》

《中国康复理论与实践》

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完全性骶上脊髓损伤后神经源性膀胱大鼠模型的建立及尿流动力学分析①

许明1a,1b，张泓1a，刘继生1a，张健1a，尹秀婷1a，张雨辰2   

  1. 1. 湖南中医药大学，a. 针灸推拿学院，b. 中医诊断学湖南省重点实验室，湖南长沙市410208；2. 湖南中医药大学第二附属医院，湖南长沙市410208。
  • 出版日期:2016-08-25 发布日期:2016-09-22

Establishment of Rat Model for Neurogenic Bladder after Complete Suprasacral Spinal Cord Injury and Urodynamic Analysis

XU Ming1a,1b, ZHANG Hong1a, LIU Ji-sheng1a, ZHANG Jian1a, YIN Xiu-ting1a, ZHANG Yu-chen2   

  1. 1. a. Department of Acupuncture, Moxibustion and Tuina; b. Hunan Provincial Key Laboratory of Diagnostics in Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410208, China; 2. The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410208, China
  • Published:2016-08-25 Online:2016-09-22

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摘要： 目的建立完全性骶上脊髓损伤后神经源性膀胱大鼠模型的制作方法，并进行尿流动力学分析和评价。方法40 只成年Sprague-Dawley 大鼠随机分为对照组(n=10)和实验组(n=30)。实验组采用改良的脊髓横断法制作T10脊髓损伤模型，术后采用Crede 手法辅助排尿并记录尿量，记录单天成模大鼠只数。模型稳定后对所有大鼠进行尿流动力学检测，HE染色观察膀胱的组织形态学差异。结果实验组大鼠死亡4 只。模型大鼠尿量在术后第18 天减少至相对稳定水平，术后21 d 模型成功24 只。实验组膀胱最大容量小于对照组(t=3.150, P=0.021)，膀胱基础压力、膀胱最大压力、漏尿点压力明显大于对照组(t>3.125, P<0.01)，膀胱顺应性明显小于对照组(t=5.330, P=0.002)。结论脊髓横断法完全性骶上脊髓损伤可复制痉挛性神经源性膀胱大鼠模型。

关键词: 骶上脊髓损伤, 神经源性膀胱, 动物模型, 尿流动力学, 大鼠

Abstract: Objective To establish the rat model for neurogenic bladder after complete suprasacral spinal cord injury, and analyze the urodynamics. Methods Forty adult Sprague-Dawley rats were randomly divided into control group (n=10) and experimental group (n=30). The spinal cord injury model on T10 was made with modified spinal cord transaction. The Crede's method was used to urinate assistantly after modeling. The amounts of urine and successful rat model everyday were recorded. The urodynamics was measured in both groups, and the morphology was observed with HE staining. Results Four rats died in the experimental group. The amount of urine was the least and stable on the 18th day, and 24 rats were successfully modeled on the 21st day. Compared with the control group, maximum bladder capacity decreased (t=3.150, P=0.021), bladder base pressure, maximum bladder pressure and leak point pressure increased (t>3.125, P<0.01), and bladder compliancy decreased (t=5.330, P=0.002) in the experimental group. Conclusion The complete suprasacral after spinal cord injury of neurogenic bladder in a rat model of manufacturing method is of high reliability, good repeatability, and low coefficient of variable characteristics for spastistic neurogenic bladder basic experimental studies.

Key words: suprasacral spinal cord injury, neurogenic bladder, animal model, urodynamics, rats