《中国康复理论与实践》

《中国康复理论与实践》

• 专题 • 上一篇    下一篇

督脉电针对不同时间段脊髓损伤大鼠运动功能及p75 神经营养素受体表达的影响①

吕威1a，莫雨平2，李冰1b，姚海江3,4，景泉凯1a，宋良玉1a，王鑫1a，毛颖秋1c，李志刚1a，时素华5   

  1. 吕威(1988-)，男，回族，河南驻马店市人，硕士研究生，主要研究方向：针刺干预中枢神经系统损伤的机理研究。
  • 出版日期:2016-08-25 发布日期:2016-09-22

Effects of Governor Vessel Electroacupuncture in Different Time Points on Motor Functions and p75 Neurotrophin Receptor after Spinal Cord Injury

LÜ Wei1a, MO Yu- ping2, LI Bing1b, YAO Hai- jiang3,4, JING Quan- kai1a, SONG Liang- yu1a, WANG Xin1a, MAO Ying-qiu1c, LI Zhi-gang1a, SHI Su-hua5   

  1. 1.a. Department of Acupuncture and Massage, b. Department of Basic Medicine, c. Scientific Research and Test Center, Beijing University of Chinese Medicine, Beijing 100029, China; 2. The Rehabilitation Department of the Third People's Hospital of Shenzhen City, Shenzhen, Guangdong 518112, China; 3. Treatment Center of Traditional Chinese Medicine, Beijing Bo'ai Hospital, China Rehabilitation Research Center, Beijing 100068, China; 4. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China; 5. Department of Rehabilitation, The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China
  • Published:2016-08-25 Online:2016-09-22

PDF (PC)

摘要： 目的观察督脉电针对脊髓损伤大鼠不同时间段运动功能及p75 神经营养素受体(p75NTR)表达的影响。方法雄性清洁级Sprague-Dawley 大鼠180 只，随机分为术后1 d、3 d、7 d 组，每个时间段组再随机分为空白组、空白电针组、假手术组、模型组和督脉电针组，每组12 只。采用改良Allen 打击法复制脊髓损伤模型。空白电针组、督脉电针组选取大椎、命门进行电针干预。采用BBB评分法评估大鼠后肢运动神经功能的变化；采用Western blotting 法检测p75NTR的表达情况。结果BBB评分结果显示，模型组、督脉电针组评分均低于其他三组；术后7 d，督脉电针组评分显著高于模型组(t=-4.510, P<0.001)。督脉电针组各时间点p75NTR表达明显低于模型组(P<0.01)。结论脊髓损伤后受损脊髓组织中p75NTR蛋白表达升高；督脉电针可以提高脊髓损伤大鼠的运动功能，下调受损脊髓组织中p75NTR的表达。

关键词: 脊髓损伤, 督脉, 电针, p75 神经营养素受体, 凋亡, 大鼠

Abstract: Objective To explore the effect of Governor Vessel electroacupuncture in different time points on motor function and p75 neurotrophin receptor (p75NTR) after spinal cord injury. Methods A total of 180 adult male Sprague-Dawley rats were randomly divided into three groups (one day, three days and seven days after modeling), and each group was divided into normal control group, normal electroacupuncture group, sham operation group, model group and Governor Vessel electroacupuncture group with 12 cases in each group. The spinal cord injury model was established with the modified Allen's method. The normal electroacupuncture group and the Governor Vessel electroacupuncture group received electroacupuncture on Dazhui (DU14) and Mingmeng (DU04) acupoints. Basso-Beattic-Bresnahan (BBB) Scale was performed to assess the motor function of rats. The expression of p75NTR was detected with Western blotting. Results The BBB score of the model group and the Governor Vessel electroacupuncture group were significantly lower than that of the other three groups. The BBB score was significantly higher in the Governor Vessel electroacupuncture group than in the model group seven days after intervention (t=-4.510, P<0.001). The expression of p75NTR was siginificantly lower in the Governor Vessel electroacupuncture group than in the model group (P<0.01). Conclusion The expression of p75NTR increased after spinal cord injury. Governor Vessel electroacupuncture could improve the motor function, and inhibit the p75NTR expression of damaged spinal cord tissues.

Key words: spinal cord injury, Governor Vessel, electroacupuncture, p75 neurotrophin receptor, apoptosis, rats