《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

电针对慢性应激抑郁模型大鼠海马磷酸二酯酶4及其亚型表达的影响①

景泉凯1，赛音朝克图2，姚海江3,4，吕威1，奥村悠斗1，杨利娟1，刘俊彤1，李志刚1   

  1. 1. 北京中医药大学针灸推拿学院，北京市100029；2. 内蒙古自治区国际蒙医医院，内蒙古呼和浩特市010065；3. 中国康复研究中心北京博爱医院康复科，北京市100068；4. 首都医科大学康复医学院，北京市100068。
  • 出版日期:2016-09-25 发布日期:2016-12-05

Effect of Electroacupuncture on Expression of Phosphodiesterase 4 and Subtypes in Hippocampus in Rats with Chronic Stress Depression

JING Quan-kai1, SAIYIN Chao-ke-tu2, YAO Hai-jiang3,4, LÜ Wei1, AOCUN You-dou1, YANG Li-juan1, LIU Jun-tong1, LI Zhi-gang1   

  1. 1. Department of Acupuncture and Massage, Beijing University of Chinese Medicine, Beijing 100029, China; 2. Inner Mongolia International Mongolian Hospital Traditional Therapy Technique, Hohhot, Inner Mongolia 010065, China; 3. Treatment Center of Traditional Chinese Medicine, Beijing Bo'ai Hospital, China Rehabilitation Research Center, Beijing 100068, China; 4. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China
  • Published:2016-09-25 Online:2016-12-05

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摘要： 目的探讨电针治疗抑郁症的生物学机制。方法成年雄性Sprague-Dawley 大鼠32 只随机分为空白组(n=8)、模型组(n=8)、电针组(n=8)、氟西汀组(n=8)，采用慢性不可预知性应激刺激结合孤养的方式建立抑郁模型。开野试验观察大鼠行为学变化。RT-PCR法检测大鼠海马磷酸二酯酶4(PDE4)A、PDE4D表达。结果模型组大鼠开野试验水平运动次数和垂直运动次数较空白组显著降低(P<0.001)，电针组和氟西汀组较模型组显著增加(P<0.001)。PDE4A、PDE4D mRNA表达，电针组比模型组显著减少(P<0.001)。结论抑制PDE4A、PDE4D表达可能是电针抗抑郁作用的机制之一。

关键词: 抑郁, 电针, 慢性应激, 磷酸二酯酶4, 大鼠

Abstract: Objective To explore the biological mechanisms of electroacupuncture (EA) for depression. Methods Forty male adult Sprague-Dawley rats were randomly divided into control group (n=8), model group (n=8), EA group (n=8) and fluoxetine group (n=8). Depressive models were established with lonely raising and chronic unpredictable mild stress. They were tested with Open-field Test, and the expression of phosphodiesterase 4 (PDE4)A and PDE4D in hippocampus was detected with RT-PCR. Results The cross and rear scores were significantly lower in the model group than in the control group (P<0.001), while it increased in the EA group and the fluoxetine group (P<0.001). Compared with the model group, the expression of PDE4A and PDE4D decreased in the EA group (P<0.001). Conclusion Electroacupuncture may relieve depression through inhibiting the expression of PDE4A and PDE4D.

Key words: depression, electroacupuncture, chronic stress, phosphodiesterase 4, rats