《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

Ski 在大鼠脊髓损伤后不同时间的表达变化①

周开升1,2，朱彦东1,2，赵鑫1,2，郭永强1,2，寇江力1,2，汪静2，李森3，龙在云3，伍亚民3，张海鸿1   

  1. 1. 兰州大学第二医院骨科，甘肃兰州市730030；2. 甘肃省骨关节疾病研究重点实验室，甘肃兰州市730030；3. 第三军医大学大坪医院野战外科研究所三室，创伤、烧伤与复合伤国家重点实验室，重庆市400042。
  • 出版日期:2016-09-25 发布日期:2016-12-05

Expression and Change of Ski after Spinal Cord Injury in Rats

ZHOU Kai- sheng1,2, ZHU Yan- dong1,2, ZHAO Xin1,2, GUO Yong- qiang1,2, KOU Jiang- li1,2, WANG Jing1,2, LI Sen3, LONG Zai-yun3, WU Ya-min3, ZHANG Hai-hong1   

  1. 1. Department of Orthopedics, Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, China; 2. Key Laboratory of Orthopedics of Gansu Province, Lanzhou, Gansu 730030, China; 3. State Key Laboratory of Trauma, Burns and Combined Injury, the Third Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China
  • Published:2016-09-25 Online:2016-12-05

PDF (PC)

摘要： 目的探究ski 在大鼠正常及损伤后脊髓中的表达及随时间变化的规律。方法60 只成年雌性Sprague-Dawley 大鼠，随机分为假手术组(n=30)和损伤组(n=30)，各组设1 周、2 周、4 周、8 周、12 周亚组，每个亚组6 只大鼠。用Allen 法制备T10打击损伤模型。损伤后1 d、3 d、1 周、2 周、4 周、8 周、12 周行BBB后肢功能评分；术后1 周、2 周、4 周、8 周、12 周，各组取3 只大鼠行HE染色，观察损伤后脊髓病理变化及空洞形成；另3 只大鼠行ski 免疫荧光染色及半定量分析。结果损伤组各时间点BBB评分均低于假手术组(P<0.05)。损伤后1 周、2 周，脊髓主要以坏死为主；4 周时空洞完全形成，空洞周围有致密瘢痕组织；8周、12 周空洞及瘢痕无明显变化，但损伤中心及附近脊髓明显变细。ski 在正常脊髓中表达较低，损伤后ski 表达逐渐增高，8 周时达到高峰，12 周时有所降低；ski 在正常及损伤后12 周脊髓中主要分布于白质；损伤后2 周、4 周和8 周时灰质中出现明确ski 表达。在损伤中心，ski 在空洞周围密集表达。结论ski 在脊髓损伤后表达。它可能作用于星形胶质细胞，并调控其活化、增生以及胶质瘢痕形成。

关键词: 脊髓损伤, ski, 星形胶质细胞, 瘢痕, 大鼠

Abstract: Objective To explore the expression and the changes of ski with time in the injured spinal cord in rats. Methods Sixty adult female Sprague-Dawley rats were randomly divided into sham group (n=30) and injury group (n=30), each group were further divided into 1 week, 2 weeks, 4 weeks, 8 weeks and 12 weeks subgroups, with 6 rats in each subgroup. Spinal cord injury at T10 was established with modified Allen's technique (10 g×25 mm) in the injury group. The hindlimbs behavior of rats was rated with Basso-Beattie-Bresnahan (BBB) scores 1 day, 3 days, 1 week, 2 weeks, 4 weeks, 8 weeks and 12 weeks after spinal cord injury. Three rats in each subgroup were stained with HE staining to observe the pathological changes of the spinal cord and the formation of cavity. The other 3 rats were analyzed with immunofluorescence staining of ski and semi quantitative analysis. Results The BBB scores of each time point were less in the injury group than in the sham group (P<0.05). Necrosis was the major pathological change in the injury groups 1 and 2 weeks after injury; cystic cavity completely formed 4 weeks after injury, with dense scar tissue around it; there was no significant change in the cavity and scar 8 and 12 weeks after injury, however, the adjacent spinal cord was obviously thinner. Ski expressed little in the normal spinal cord, and expressed more and more after injury, peaked at 8 weeks and decreased then. Ski was mainly observed in white matter in the sham group and 12 weeks injury subgroup, which was in gray matter 2, 4 and 8 weeks after injury. Ski was highly expressed around the cavity in injury center and formed high expression band. Conclusion Ski expresses after spinal cord injury in rats, that may be associated with the activation and proliferation of astrocytes and the formation of glial scar.

Key words: spinal cord injury, ski, astrocyte, scar, rats