《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

PM2.5 对支气管上皮细胞色素上皮衍生因子表达的影响①

朱成华1,2，施丽君2，张灵凤2，计晓兰2，杜强1,2   

  1. 1. 南京医科大学第二附属医院呼吸科，江苏南京市210011；2. 南京医科大学第二临床医学院，江苏南京市210011。
  • 出版日期:2016-10-25 发布日期:2016-12-05

Effects of PM2.5 on Expression of Pigment Epithelium-derived Factor in Bronchial Epithelial Cells

ZHU Cheng-hua1, SHI Li-jun2, ZHANG Ling-feng2, JI Xiao-lan2, DU Qiang1   

  1. 1. Department of Respiration Medicine, No.2 Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, China; 2. the Second Clinical Medical School of Nanjing Medical University, Nanjing, Jiangsu 210011, China
  • Published:2016-10-25 Online:2016-12-05

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摘要： 目的探索不同浓度PM2.5 对支气管上皮细胞(BEAS-2B)色素上皮衍生因子(PEDF)蛋白表达的影响。方法BEAS-2B细胞传代培养，加入低、中、高浓度PM2.5 悬液(25 μg/ml、50 μg/ml、100 μg/ml)刺激24 h。采用酶联免疫吸附试验(ELISA)检测细胞培养上清液中PEDF 水平；Western blotting 检测BEAS-2B细胞中PEDF 蛋白表达水平。结果与对照组相比，PM2.5 浓度为25μg/ml 时，细胞内和上清液中PEDF蛋白水平均有增加趋势，但无统计学意义(t=-0.730, t=-1.840, P>0.05)；PM2.5 浓度为50 μg/ml和100 μg/ml 时，细胞内和上清液中PEDF蛋白表达水平明显增高(t>5.798, P<0.01)。结论中、高浓度PM2.5 能够增加支气管上皮细胞中PEDF蛋白水平，并呈浓度依赖性。

关键词: 细颗粒物, 支气管上皮细胞, 色素上皮衍生因子

Abstract: Objective To explore the effect of particulate matter (PM) 2.5 on the expression of pigment epithelium- derived factor (PEDF) protein in bronchial epithelial cells (BEAS-2B). Methods Human BEAS-2B were subcultivated, followed by low, medium and high concentrations of PM2.5 (25 μg/ml, 50 μg/ml, 100 μg/ml) stimulation for 24 hours. The expression of PEDF protein in supernatant was analyzed by enzyme-linked immunosorbent assay (ELISA), and the expression in BEAS-2B cells was detected by Western blotting. Results Compared with the control group, the expression of PEDF protein in supernatant and BEAS-2B cells induced by PM2.5 (25 μg/ml) increased, but no significance was found (t=-0.730, t=-1.840, P>0.05), and the expression induced by PM2.5 with the concentrations of 50 μg/ml and 100 μg/ml significantly increased (t>5.798, P<0.05). Conclusion PM2.5 with the concentrations of 50 μg/ml and 100 μg/ml could increase the expression of PEDF protein in a concentration-dependent manner both in supernatant and BEAS-2B cells.

Key words: particulate matter, bronchial epithelial cells, pigment epithelium-derived factor