《中国康复理论与实践》

《中国康复理论与实践》

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电针委中穴对大鼠退变腰椎间盘组织中Bcl-xL、Bax表达的影响①

何挺超1，陈少清1，林建平2，刘午龙1，夏梦1,3，王诗忠1   

  1. 1. 福建中医药大学，福建福州市350122；2. 福建中医药大学附属康复医院，福建福州市350003；3. 福建省康复技术重点实验室，福建福州市350122。
  • 出版日期:2016-11-25 发布日期:2016-12-05

Effects of Electroacupuncture at Weizhong on Expression of Bcl-xL and Bax in Rats with Lumbar Disc Degeneration

HE Ting-chao1, CHEN Shao-qing1, LIN Jian-ping2, LIU Wu-long1, XIA Meng1,3, WANG Shi-zhong1   

  1. 1. Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China; 2. Fujian University of Traditional Chinese Medicine Subsidiary Rehabilitation Hospital, Fuzhou, Fujian 350003, China; 3. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350122, China
  • Published:2016-11-25 Online:2016-12-05

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摘要： 目的观察电针委中穴对大鼠腰椎间盘退变组织中抗凋亡因子Bcl-xL、凋亡因子Bax蛋白表达的影响。方法30 只雄性Sprague-Dawley 大鼠随机分为假手术组(n=10)、模型组(n=10)和电针委中组(n=10)。模型组和电针委中组通过纤维环穿刺法建立大鼠腰椎间盘退变模型。造模成功1 个月后，电针委中组予电针委中穴，连续4 周。HE染色观察组织形态，免疫组化法观察退变腰椎间盘组织中Bax 蛋白表达，Western blotting 检测Bcl-xL、Bax 蛋白表达。结果HE染色显示，腰椎间盘组织退变程度由轻至重依次是假手术组、电针委中组、模型组。模型组Bcl-xL 蛋白表达量低于假手术组(P<0.05)，Bax 蛋白表达量明显高于假手术组(P<0.01)；电针委中组Bcl-xL 蛋白表达量显著高于模型组(P<0.001)，Bax 蛋白表达量明显低于模型组(P<0.01)。结论电针委中穴治疗腰椎间盘退变，可能与上调Bcl-xL蛋白表达并抑制Bax蛋白表达有关。

关键词: 腰椎间盘退变, 电针, 委中穴, 凋亡因子, 大鼠

Abstract: Objective To explore the effect of electroacupuncture at Weizhong (BL40) acupoint on the expression of Bcl-xL and Bax in rats with lumbar disc degeneration. Methods Thirty male adult Sprague-Dawley rats were randomly divided into sham-operated group (n= 10), model group (n=10), and electroacupuncture group (n=10). The lumbar disc degeneration model was established by puncturing the annulus fibrosis in the model group and electroacupuncture group. One month after modeling, the electroacupuncture group was electroacupunctured at Weizhong acupoint for four weeks. HE staining was used to observe the pathological changes. Immunohistochemical staining was used to observe the expression of Bax in the lumbar disc tissue. Western blotting was used to detect the expression of Bcl-xL and Bax protein. Results HE staining showed that the degrees of lumbar disc degeneration from light to severe was ranged as: the sham-operated group, the electroacupuncture group and the model group. The expression of Bcl-xL was lower (P<0.05), and the expression of Bax was higher (P<0.01) in the model group than in the sham-operated group, which was opposite (P<0.01) in the electroacupuncture group than in the model group. Conclusion Electroacuponcture at Weizhong may upregulate the expression of Bcl-xL and downregulate the expression of Bax in rats with lumbar disc degeneration.

Key words: lumbar disc degeneration, electroacupuncture;Weizhong, apoptosis factor, rats