《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

黄芪多糖对大鼠脊髓损伤后运动功能和脊髓病理的效果①

郑利强1，伍亚民2，石永江3，江琼1   

  1. 1. 重庆医科大学，重庆市400016；2. 第三军医大学大坪医院野战外科研究所三室，创伤烧伤与复合伤国家重点实验室，重庆市400042；3. 解放军后勤工程学院门诊部，重庆市401331。
  • 出版日期:2016-11-25 发布日期:2016-12-05

Effect of Astragalus Polysaccharide on Motor Function and Pathology after Spinal Cord Injury in Rats

ZHENG Li-qiang1, WU Ya-min2, SHI Yong-jiang3, JIANG Qiong1   

  1. 1. Chongqing Medical University, Chongqing 400016, China; 2. Third Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China; 3. Logistical Engineering University of PLA Hospital, Chongqing 401331, China
  • Published:2016-11-25 Online:2016-12-05

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摘要： 目的观察黄芪多糖对脊髓损伤后运动功能和脊髓病理变化的影响。方法70 只成年Sprague-Dawley 大鼠分为正常组(n=10)、损伤组(n=30)和治疗组(n=30)，损伤组和治疗组内再分为7 d、14 d、28 d 三个亚组。损伤组和治疗组均采用Allen 法(10g×25 mm)损伤大鼠T10脊髓。造模成功后，治疗组术后每天腹腔注射黄芪多糖注射液10 mg/kg。术后7 d、14 d、28 d，各亚组分别行BBB评分，HE染色观察细胞形态，Nissl 染色观察尼氏小体，铬花青染色观察脱髓鞘情况。结果术后各时间点，治疗组BBB评分高于损伤组(P<0.05)。损伤组术后7 d、14 d 损伤部位以坏死组织为主，空洞开始形成；28 d 时空洞基本形成，空洞周围有致密瘢痕。治疗组各时间点损伤部位坏死较轻，14 d、28 d 时空洞面积缩小。损伤组术后7 d 出现脱髓鞘，并逐渐加重；术后28 d，损伤中心出现空洞。治疗组各时间点脱髓鞘及髓鞘排列疏松情况有所改善。损伤组术后7 d 尼氏小体开始融合，14 d 时融合加重，28 d 时几乎完全融合或溶解。治疗组各时间点尼氏小体融合程度较轻。结论黄芪多糖能减轻脊髓损伤后病理损害，促进运动功能改善。

关键词: 脊髓损伤, 黄芪多糖, 运动功能, 病理, 大鼠

Abstract: Objective To observe the effect of astragalus polysaccharide on motor function and pathology after spinal cord injury in rats. Methods Seventy adult Sprague-Dawley rats were selected as normal group (n=10), injury group (n=30) and treatment group (n=30), and the injury group and the treatment group were divided into 7 days, 14 days, 28 days subgroups. The injury group and the treatment group were modeled with Allen's mode at T10 (10 g×25 mm). The treatment group was injected with astragalus polysaccharide 10 mg/kg per day after injury. They were rated with Basso-Beattie-Bresnahan (BBB) scores 7 days, 14 days and 28 days after injury, while the morphology was observed with HE staining, Nylon body was observed with Nissl staining, and myelin sheath was observed with eriochrome cyanine staining. Results The BBB score was significantly higher in the treatment group than in the injury group at each time point (P<0.05). There was a large number of necrotic tissue in the injured cords and cystic cavity began to form 7 and 14 days after injury. Cystic cavity formed basically and surrounded with dense scar 28 days after injury. The necrosis and cystic cavity alleviated in the treatment group at each time point. Demyelination and myelin sheaths loose were found 7 days after injury, and aggravated with the time. There was a cystic cavity in trauma center 28 days after injury. The demyelination and myelin sheaths loose relieved at each time point in the treatment group. Nissl bodies began to coalesce 7 days after injury, and aggravated 14 days after injury, coalesced completely 28 days after injury. Nissl body coalesced alleviatively in the treatment group at each time point. Conclusion Astragalus polysaccharide may reduce the damage and promote the recovery of motor function after spinal cord injury in rats.

Key words: spinal cord injury, astragalus polysaccharide, motor function, pathology, rats