针康法对脑缺血大鼠神经功能和细胞外信号调节激酶1/2信号通路的影响

doi:10.3969/j.issn.1006-9771.2017.01.007

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (1): 27-31.doi: 10.3969/j.issn.1006-9771.2017.01.007

针康法对脑缺血大鼠神经功能和细胞外信号调节激酶1/2信号通路的影响

唐强¹, 叶涛², 朱路文¹, 吴孝军², 李宏玉², 田源²

1.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001;
2.黑龙江中医药大学,黑龙江哈尔滨市 150040。

收稿日期:2016-10-10 出版日期:2017-01-20 发布日期:2017-02-17
作者简介:唐强(1963-),男,汉族,四川大竹县人,博士,教授,主要研究方向：神经系统疾病中医康复基础与临床研究。E-mail: tangqiang1963@163.com。
基金资助:
国家自然科学基金项目(No.81273818; No.81473762); 黑龙江中医药大学领军人才计划项目(No.2012RCL02)

Effects of Acupuncture-rehabilitation Therapy on Neurological Function and Extracellular Signal-regulated Kinase 1/2 Signaling Pathway after Focal Cerebral Ischemia in Rats

TANG Qiang¹, YE Tao², ZHU Lu-wen¹, WU Xiao-jun², LI Hong-yu², TIAN Yuan²

1. Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China;
2. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China

Received:2016-10-10 Published:2017-01-20 Online:2017-02-17
Contact: TANG Qiang. E-mail: tangqiang1963@163.com

摘要/Abstract

摘要： 目的探讨针康法对脑缺血大鼠神经功能预后和细胞外信号调节激酶1/2 (ERK1/2)信号通路的影响。方法 90只雄性Sprague-Dawley大鼠随机分入假手术组(n=18)、模型组(n=18)、针刺组(n=18)、康复组(n=18)及针康组(n=18),再分为术后3 d、7 d、14 d三个亚组(n=6)。线栓法制备永久性局灶性脑缺血模型。假手术组和模型组不进行治疗,针刺组采用头穴丛刺针法治疗,康复组给予电动跑台训练,针康组采用针康法治疗。各时间点,采用改良神经损害严重程度评分进行评定,Western blotting法检测缺血半暗区皮层ERK1/2、p-ERK1/2蛋白表达。结果术后各时间点,与模型组比较,各治疗组神经缺损评分降低(P<0.05);术后7 d、14 d,与针刺组、康复组比较,针康组评分降低(P<0.05)。术后各时间点,与模型组比较,各治疗组p-ERK1/2蛋白表达增加(P<0.05),(p-ERK1/2)/(ERK1/2)增加(P<0.05);与针刺组、康复组比较,针康组p-ERK1/2蛋白表达升高(P<0.05),(p-ERK1/2)/(ERK1/2)增加(P<0.05)。结论针康法治疗可进一步改善脑缺血大鼠神经行为,可能与ERK1/2信号通路激活有关。

关键词: 脑缺血, 针康法, 神经功能, 细胞外信号调节激酶1/2, 大鼠

Abstract: Objective To explore the effects of acupuncture-rehabilitation therapy on neurological function recovery and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway activation after permanent focal cerebral ischemia in rats. Methods Ninty male Sprague-Dawley rats were divided into five groups, namely sham group, model group, acupuncture group, rehabilitation group and acupuncture-rehabilitation group, and 18 in each group. Each group was further divided into 3 days, 7 days and 14 days subgroups (n=6). Their middle cerebral arteries were occluded except those of sham group. The sham and model groups accepted no treatment, while the acupuncture group accepted cluster needling of scalp acupuncture, rehabilitation group accepted treadmill training, and the acupuncture-rehabilitation group accepted both acupuncture and treadmill training. They were assessed with modified Neurologic Severity Score (mNSS) 3, 7 and 14 days after modeling, while the expression of ERK1/2 and p-ERK1/2 were determined with Western blotting. Results The neurologic severity score reduced in all three treatment groups (P<0.05) compared with that of the model group at every time point, and was the least in the acupuncture-rehabilitation group (P<0.05) 7 and 14 days after modeling among the treatment groups. Meanwhile, the expression of p-ERK1/2 increased in all three treatment groups (P<0.05), and was the most in the acupuncture-rehabilitation group (P<0.05), as well as the rate of (p-ERK1/2)/(ERK1/2) (P<0.05). Conclusion Acupuncture-rehabilitation therapy can promote the neurological function recovery, which may be associated with activation of ERK1/2 signaling pathway.

Key words: cerebral ischemia, acupuncture-rehabilitation therapy, neurological function, extracellular signal-regulated kinase 1/2, rats

中图分类号:

R743.3

唐强, 叶涛, 朱路文, 吴孝军, 李宏玉, 田源. 针康法对脑缺血大鼠神经功能和细胞外信号调节激酶1/2信号通路的影响[J]. 《中国康复理论与实践》, 2017, 23(1): 27-31.

TANG Qiang, YE Tao, ZHU Lu-wen, WU Xiao-jun, LI Hong-yu, TIAN Yuan. Effects of Acupuncture-rehabilitation Therapy on Neurological Function and Extracellular Signal-regulated Kinase 1/2 Signaling Pathway after Focal Cerebral Ischemia in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(1): 27-31.

参考文献

[1] Meschia JF, Bushnell C, Boden-Albala B, et al. Guidelines for the Primary Prevention of Stroke A Statement for Healthcare Professionals from the American Heart Association/American Stroke Association [J]. Stroke, 2014, 45(12): 3754-3832.
[2] 唐强,朱路文. 脑卒中康复新策略——针康法[J]. 中国康复医学杂志, 2015, 30(10): 1071-1073.
[3] 张荣天,雷鸣,刘伟,等. Hif-1α及ERK信号通路在缺血再灌注损伤组织中的表达研究现状[J]. 世界最新医学信息文摘(电子版), 2015, 15(19): 23-24.
[4] Yenari MA, Han HS. Neuroprotective mechanisms of hypothermia in brain ischaemia [J]. Nat Rev Neurosci, 2012, 13(4): 267-278.
[5] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989, 20(1): 84-91.
[6] 唐强,姜云飞,朱路文,等. 针康法对局灶性脑缺血大鼠神经功能缺损及缺血区碱性成纤维细胞生长因子、血管抑制素蛋白表达的影响[J]. 中国康复理论与实践, 2015, 21(10): 1151-1155.
[7] Li Y, Chopp M, Chen J, et al. Intrastriatal transplantation of bone marrow nonhematopoietic cells improves functional recovery after stroke in adult mice [J]. J Cereb Blood Flow Metab, 2000, 20(9): 1311-1319.
[8] 唐强,朱冬梅,刘景隆,等. 头穴丛刺结合康复治疗急性脑梗死患者运动功能障碍的临床观察[J]. 中国康复理论与实践, 2004, 10(11): 697-698.
[9] 张慧敏,唐强,朱路文. 针康法对脑卒中运动性失语患者抑郁的影响 [J]. 中国康复理论与实践, 2011, 17(4): 319-321.
[10] 王艳,张立,李淑荣,等. 头穴丛刺结合认知训练对脑梗死患者认知功能障碍的影响[J]. 中国康复理论与实践, 2011, 17(4): 316-318.
[11] 关莹,张立,邢艳丽,等. 针康法对脑卒中后痉挛状态的影响[J]. 中国康复理论与实践, 2011, 17(4): 325-327.
[12] 唐强,朱路文,邢艳丽,等. 针康法对脑缺血大鼠运动功能及GAP-43、Nogo-A表达的影响[J]. 针灸临床杂志, 2015, 31(1): 54-57.
[13] 唐强,王宏宇,王艳,等. 针康法对局灶性脑缺血大鼠运动功能及缺血区周围内皮素受体-β表达的影响[J]. 针灸临床杂志, 2013, 29(5): 65-68.
[14] 孔妍. 针康法对脑缺血大鼠Caspase-3 mRNA和蛋白表达的影响[J]. 针灸临床杂志, 2013, 29(5): 63-65.
[15] 孙妲男,唐强,郑剑. 针康法对糖尿病缺血性脑卒中大鼠外周血清NF-κB-p65、TNF-α表达的影响[J]. 针灸临床杂志, 2014, 30(8): 60-63.
[16] Maddahi A, Edvinsson L. Cerebral ischemia induces microvascular pro-inflammatory cytokine expression via the MEK/ERK pathway [J]. J Neuroinflammation, 2010, 7: 14.
[17] Qiao H, Zhang X, Zhu C, et al. Luteolin downregulates TLR4, TLR5, NF-κB and p-p38MAPK expression, upregulates the p-ERK expression, and protects rat brains against focal ischemia [J]. Brain Res, 2012, 1448: 71-81.
[18] Rabenstein M, Vay SU, Flitsch LJ, et al. Osteopontin directly modulates cytokine expression of primary microglia and increases their survival [J]. J Neuroimmunol, 2016, 299: 130-138.
[19] Singh N, Sharma G, Mishra V, et al. Hypoxia inducible factor-1: its potential role in cerebral ischemia [J]. Cell Mol Neurobiol, 2012, 32(4): 491-507.
[20] Xie G, Yang S, Chen A, et al. Electroacupuncture at Quchi and Zusanli treats cerebral ischemia?reperfusion injury through activation of ERK signaling [J]. Exp Ther Med, 2013, 5(6): 1593-1597.

针康法对脑缺血大鼠神经功能和细胞外信号调节激酶1/2信号通路的影响

Effects of Acupuncture-rehabilitation Therapy on Neurological Function and Extracellular Signal-regulated Kinase 1/2 Signaling Pathway after Focal Cerebral Ischemia in Rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	李芳, 霍速, 杜巨豹, 刘秀贞, 李小爽, 宋为群. 经颅直流电刺激联合任务导向性康复训练对脊髓损伤大鼠前肢运动障碍的效果[J]. 《中国康复理论与实践》, 2023, 29(7): 777-781.
[2]	康晓宇, 刘丽旭, 王文竹, 王云雷. 普拉克索联合左旋多巴对全脑缺血再灌注损伤大鼠认知能力及线粒体功能的影响[J]. 《中国康复理论与实践》, 2023, 29(5): 533-540.
[3]	罗兰, 李璐, 金沐. 氙气后处理对脊髓缺血再灌注损伤的效果：Akt信号通路和自噬机制[J]. 《中国康复理论与实践》, 2023, 29(2): 174-181.
[4]	朱烈烈, 夏建宁, 蒲新宇, 邵湘芝, 张嘉诚, 吴登宠. 老年营养风险指数对脑卒中后神经功能预后的效能[J]. 《中国康复理论与实践》, 2023, 29(11): 1346-1352.
[5]	黄志霖, 徐发邵, 施静, 黄淦, 刘梅芳, 张霞辉. 线栓法建立卒中后吞咽障碍的大鼠模型[J]. 《中国康复理论与实践》, 2023, 29(10): 1147-1153.
[6]	秦彦强, 董浩, 孙迎春, 程先宽, 姚海江. 不同针刺方案对卒中后抑郁大鼠神经递质及相关炎性因子的影响[J]. 《中国康复理论与实践》, 2023, 29(1): 30-37.
[7]	缪培,张通,米海霞,张伟东. 不同线栓法复制局灶性脑缺血模型大鼠恢复期学习记忆能力的差异及其机制[J]. 《中国康复理论与实践》, 2022, 28(7): 789-796.
[8]	周小珏,冯婧,庞日朝,刘捷,张安仁. 隔日限食减轻脊髓损伤大鼠炎症反应的芳香烃受体/细胞因子信号传导抑制因子2/核转录因子-κB信号通路机制[J]. 《中国康复理论与实践》, 2022, 28(5): 544-551.
[9]	宋绍霏,侯园园,王云雷,张通. 异常光周期所致昼夜节律紊乱对大鼠腓肠肌时钟基因和葡萄糖摄取相关基因表达节律的影响[J]. 《中国康复理论与实践》, 2022, 28(5): 552-558.
[10]	李童,方志鹏,邵玉萍,王平. 有氧运动对睡眠剥夺大鼠学习记忆及海马神经元突触可塑性的效果[J]. 《中国康复理论与实践》, 2022, 28(11): 1270-1277.
[11]	唐强,王雪,穆姿辰,张世强,朱路文. 针康法促进脑卒中后下肢运动功能重建的功能性近红外光谱研究[J]. 《中国康复理论与实践》, 2022, 28(1): 32-37.
[12]	王静怡,尹杰,刘建成,庞日朝,王文春. 蜘蛛香环烯醚萜类成分对急性脊髓损伤大鼠神经细胞焦亡的影响[J]. 《中国康复理论与实践》, 2021, 27(6): 653-660.
[13]	周文美,陶陶,吴霜,王廷龙,杨正奕,张莹. 丰富环境对脑缺血再灌注损伤大鼠神经功能和缺血半暗带区葡萄糖代谢的影响[J]. 《中国康复理论与实践》, 2021, 27(5): 522-529.
[14]	王琼芬,王风波,王科,钟永强,王娇娇. 电针风池对急性脑梗死大鼠脑星形胶质细胞和神经元的保护效果[J]. 《中国康复理论与实践》, 2021, 27(3): 302-309.
[15]	李向哲,丁洁,王庆华,董传明,王彤,吴勤峰. 减重平板训练对脊髓损伤大鼠神经病理性疼痛及脊髓后角谷氨酸脱羧酶-65/67表达的影响[J]. 《中国康复理论与实践》, 2021, 27(2): 131-136.