丰富环境对小鼠缺血性脑损伤后运动、社交功能的作用

doi:10.3969/j.issn.1006-9771.2017.01.008

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (1): 32-36.doi: 10.3969/j.issn.1006-9771.2017.01.008

丰富环境对小鼠缺血性脑损伤后运动、社交功能的作用

姜敏¹, 王继先², 余波¹, 杨国源³, 陈文华¹

1.上海交通大学附属第一人民医院康复医学科,上海市 200080;
2.上海交通大学医学院附属瑞金医院康复医学科,上海市 200025;
3.上海交通大学Med-X研究院,上海市 200030。

收稿日期:2016-07-15 出版日期:2017-01-20 发布日期:2017-02-17
作者简介:姜敏(1988-),女,汉族,上海市人,硕士研究生,主要研究方向：神经康复。通讯作者：陈文华,女,汉族,主任医师,教授。E-mail: chen.wh@163.com。
基金资助:
上海市科委产学研医项目(No.11DZl921307)

Effect of Enriched Environment on Motor and Social Function after Ischemic Brain Injury in Mice

JIANG Min¹, WANG Ji-xian², YU Bo¹, YANG Guo-yuan³, CHEN Wen-hua¹

1. Department of Rehabilitation Medicine, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, China;
2. Department of Rehabilitation Medicine, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;
3. Med-X Research Institute, Shanghai Jiaotong University, Shanghai 200030, China

Received:2016-07-15 Published:2017-01-20 Online:2017-02-17
Contact: CHEN Wen-hua. E-mail: chen.wh@163.com

摘要/Abstract

摘要： 目的研究长期丰富环境对促进小鼠缺血性脑损伤后运动、社交功能恢复的作用。方法成年雄性ICR小鼠16只,复制大脑中动脉永久性栓塞模型。术后第1天将小鼠分为丰富环境组(n=8)和标准环境组(n=8)。术后7 d、14 d、21 d、28 d对小鼠进行改良神经损害严重程度评分(mNSS),进行疲劳转棒仪、聪明笼子检测。结果术后28 d,丰富环境组mNSS评分与转棒疲劳试验结果优于标准环境组(t>2.927, P<0.05)。一般运动试验中,术后14~28 d,丰富环境组在聪明笼子中间区域出现的时间长于标准环境组(t>2.480, P<0.05);社交行为试验中,术后7~28 d,丰富环境组比标准环境组表现出对陌生小鼠更感兴趣的趋势,但无显著性差异(P>0.05);术后14~28 d,丰富环境组出现在中间区域的时间明显长于标准环境组(t>3.472, P<0.01);术后14 d在聪明笼子中的移动速度高于标准环境组(P<0.05)。结论丰富环境有助于小鼠缺血性脑损伤后运动功能恢复,并可能促进其社交功能恢复。

关键词: 缺血性脑损伤, 丰富环境, 运动, 社交, 行为, 小鼠

Abstract: Objective To explore the role of long-term enriched environment in promoting the recovery of motor and social function in mice after ischemic brain injury. Methods Sixteen adult male ICR mice underwent permanent middle cerebral artery occlusion (MCAO). The first day after operation, they were divided into enriched environment group (n=8) and standard condition group (n=8). The mice were tested with modified Neurological Severity Score (mNSS), rotarod test and smart cage 7, 14, 21, 28 days after modeling. Results The score of mNSS and the result of rotarod test improved more in the enriched environment group than in the standard condition group 28 days after MCAO (t>2.927, P<0.05). The occupancy time in the middle of smart cage was longer in the enriched environment group than in the standard condition group 7 to 28 days after MCAO (t>2.480, P<0.05) in the general move test. There was a trend of being more interested in strange mice in the enriched environment group than in the standard condition group, but the difference was not statistically significant (P>0.05) in the social behavior test; however, the occupancy time in the middle of smart cage was longer in the enriched environment group than in the standard condition group 14 to 28 days after MCAO (t>3.472, P<0.01), and the velocity of moving was higher 14 days after MCAO (P<0.05). Conclusion Enriched environment could promote the recovery of motor function, somehow of social function, in mice after ischemic brain injury.

Key words: ischemic brain injury, enriched environment, motor, sociality, behavior, mice

中图分类号:

R743.3

姜敏, 王继先, 余波, 杨国源, 陈文华. 丰富环境对小鼠缺血性脑损伤后运动、社交功能的作用[J]. 《中国康复理论与实践》, 2017, 23(1): 32-36.

JIANG Min, WANG Ji-xian, YU Bo, YANG Guo-yuan, CHEN Wen-hua. Effect of Enriched Environment on Motor and Social Function after Ischemic Brain Injury in Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(1): 32-36.

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丰富环境对小鼠缺血性脑损伤后运动、社交功能的作用

Effect of Enriched Environment on Motor and Social Function after Ischemic Brain Injury in Mice

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