次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ及Tau蛋白表达的影响

doi:10.3969/j.issn.1006-9771.2017.03.010

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (3): 298-303.doi: 10.3969/j.issn.1006-9771.2017.03.010

次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ及Tau蛋白表达的影响

蔡丽¹, 王晋², 崔尧^{3, 4}, 张鸽², 武松艳¹, 张文东¹, 刘朝晖¹

1.第四军医大学唐都医院康复医学科,陕西西安市 710038;
2.第四军医大学放射医学教研室,陕西西安市 710032;
3.中国康复研究中心北京博爱医院,北京市 100068;
4.首都医科大学康复医学院,北京市 100068。

收稿日期:2016-10-26 出版日期:2017-03-05 发布日期:2017-03-31
通讯作者: 刘朝晖(1962-),女,汉族,山西运城市人,博士,副主任医师,硕士研究生导师,主要研究方向：物理治疗的临床应用与机制研究。E-mail: 15829845741@163.com。
作者简介:蔡丽(1987-),女,汉族,陕西商南县人,硕士研究生,治疗师,主要研究方向：言语认知功能障碍的治疗及其机制。
基金资助:
国家自然科学基金项目(No.31470825)

Effects of Infrasound on Expression of Calmodulin-dependent Protein Kinase II and Tau Protein in Hippocampus of Rats

CAI Li¹, WANG Jin², CUI Yao^{3, 4}, ZHANG Ge², WU Song-yan¹, ZHANG Wen-dong¹, LIU Zhao-hui¹

1. Department of Rehabilitation Medicine, Tangdu Hospital, the Fourth Military Medicine University, Xi'an, Shaanxi 710038, China;
2. Section of Radiation Medicine Teaching & Research, the Fourth Military Medicine University, Xi'an, Shaanxi 710032, China;;
3. Beijing Bo'ai Hospital, China Rehabilitation Research Center, Beijing 100068, China;
4. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China

Received:2016-10-26 Published:2017-03-05 Online:2017-03-31
Contact: Correspondence to LIU Zhao-hui. E-mail: 15829845741@163.com

摘要/Abstract

摘要： 目的探讨8 Hz、130 dB次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ(CaMKⅡ)及tau蛋白表达的影响。方法56只雄性Sprague-Dawley大鼠随机分为对照组(n=8)、1 d组(n=8)、7 d组(n=8)、14 d组(n=32)。14 d组再分为照射后1 h、6 h、24 h、48 h亚组,每亚组8只。对照组每天次声仓内无次声作用放置2 h,其余各组8 Hz、130 dB次声照射2 h。免疫组织化学、Western blotting和ELISA方法检测各组大鼠海马区磷酸化CaMKⅡ(pT²⁸⁶-CaMKⅡ)及tau蛋白表达。结果14 d组pT²⁸⁶-CaMKⅡ水平最高(F>14.912, P<0.001),tau蛋白表达最多(F>36.229, P<0.001),7 d组次之(P<0.05)。14 d组中,次声后1 h、6 h,tau蛋白表达最高,24 h有所恢复,但仍较对照组高(P<0.05)。结论8 Hz、130 dB次声作用后,大鼠海马区CaMKⅡ磷酸化水平和tau蛋白表达及磷酸化水平增高,可能参与次声致大鼠学习记忆功能受损。

关键词: 次声, 钙调依赖性蛋白激酶Ⅱ, tau蛋白, 学习记忆, 海马, 大鼠

Abstract: ObjectiveTo study the effect of infrasound on expression of calmodulin-dependent protein kinase II (CaMKII) and tau protein in hippocampus of rats. MethodsFifty-six male Sprague-Dawley rats were randomized into control group (n=8), 1-day group (n=8), 7-day group (n=8) and 14-day group (n=32), and the 14-day group was subgrouped as 1-hour, 6-hour, 24-hour, 48-hour subgroups, naming after the time after infrasound exposure, 8 in each subgroup. All the test groups were put in an infrasound field with 8 Hz, 130 dB for 2 hours daily, while the control group was put in the infrasound instrument without infrasound exposure for 2 hours daily. The expression of pT²⁸⁶-CaMKII and tau protein in hippocampus was detected with immunohistochemisty, Western blotting and enzyme-linked immunoabsorbent assay. ResultsThe expression of pT²⁸⁶-CaMKII was the most in 14-day group (F>14.912, P<0.001), as well as the expression of tau protein (F>36.229, P<0.001), and secondary in 7-day group (P<0.05). For 14-day group, the expression of tau protein was the most in 1-hour and 6-hour subgroups, and dropped down in 24-hour subgroup, although more than that in the control group (P<0.05). ConclusionExposure of 8 Hz, 130 dB infrasound may induce phosphorylation of CaMKII and tau protein, and the expression of tau protein in hippocampal cells in rat, which may disturb their learning and memory function.

Key words: infrasound, calmodulin-dependent protein kinase II, tau protein, learning and memory, hippocampus, rats

中图分类号:

R594.9

蔡丽, 王晋, 崔尧, 张鸽, 武松艳, 张文东, 刘朝晖. 次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ及Tau蛋白表达的影响[J]. 《中国康复理论与实践》, 2017, 23(3): 298-303.

CAI Li, WANG Jin, CUI Yao, ZHANG Ge, WU Song-yan, ZHANG Wen-dong, LIU Zhao-hui. Effects of Infrasound on Expression of Calmodulin-dependent Protein Kinase II and Tau Protein in Hippocampus of Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(3): 298-303.

参考文献

[1] 章菲,杨庆生,夏雅琴,等. 次声:监测地震的新领域[J]. 地学前缘, 2013, 20(6): 94-101.
[2] Pei ZH, Chen BY, Tie R, et al. Infrasound exposure induces apoptosis of rat cardiac myocytes by regulating the expression of apoptosis-related proteins [J]. Cardiovasc Toxicol, 2011, 11(4): 341-346.
[3] 张歆薇,刘海强,徐胜龙,等. 次声作用对大鼠血浆SOD、MDA、NO水平的影响[J]. 现代生物医学进展, 2012, 12(18): 3416-3418.
[4] Lin T, Liu Y, Shi M, et al. Promotive effect of ginsenoside Rd on proliferation of neural stem cells in vivo and in vitro [J]. J Ethnopharmacol, 2012, 142(3): 754-761.
[5] Cheng H, Wang B, Tang C, et al. Infrasonic noise induces axonal degeneration of cultured neurons via a Ca 2+ influx pathway [J]. Toxicol Lett, 2012, 212(2): 190-197.
[6] Cai J, Jing D, Shi M, et al. Epigallocatechin gallate (EGCG) attenuates infrasound-induced neuronal impairment by inhibiting microglia-mediated inflammation [J]. J Nutr Biochem, 2014, 25(7): 716-725.
[7] 谭永霞,李玲,陈景藻,等. 次声对大鼠海马超微结构的影响[J]. 中国康复理论与实践, 2003, 9(11): 645-647.
[8] 费舟,章翔,王晓峰,等. 次声作用后大鼠血脑屏障的改变及意义[J]. 医学争鸣, 1999, 20(8): 678-680.
[9] Liu J, Lin T, Yan X, et al. Effects of infrasound on cell proliferation in the dentate gyrus of adult rats [J]. Neuroreport, 2010, 21(8): 585-589.
[10] 袁华,龙华,牟翔,等. 8 Hz次声对大鼠学习记忆能力和神经元再生的影响[J]. 中国康复医学杂志, 2008, 23(5): 385-387.
[11] 魏智钧,李玲,胨莆藻,等. 次声作用对大鼠记忆功能及隔内侧核和斜角带核胆碱能神经元表达的影响[J]. 中华物理医学与康复杂志, 2001, 23(2): 79-82.
[12] 江山,徐成峰,李亚娜,等. 次声诱导原代培养大鼠星形胶质细胞释放谷氨酸的研究[J]. 中国康复医学杂志, 2014, 29(6): 499-503.
[13] Du F, Yin L, Shi M, et al. Involvement of microglial cells in infrasonic noise-induced stress via upregulated expression of corticotrophin releasing hormone type 1 receptor [J]. J Neurosci Res, 2010, 167(3): 909-919.
[14] Shi M, Du F, Liu Y, et al. Glial cell-expressed mechanosensitive channel TRPV4 mediates infrasound-induced neuronal impairment [J]. Acta Neuropathol, 2013, 126(5): 725-739.
[15] 胡江平,耿瑞,李凯军. tau蛋白与阿尔茨海默病的研究进展[J]. 中国康复理论与实践, 2014, 20(11): 1031-1034.
[16] O'Day DH, Myre MA. Calmodulin-binding domains in Alzheimer's disease proteins: extending the calcium hypothesis [J]. Biochem Bioph Res Co, 2004, 320(4): 1051-1054.
[17] Liu Z, Gong L, Li X, et al. Infrasound increases intracellular calcium concentration and induces, apoptosis in hippocampi of adult rats [J]. Mol Med Rep, 2011, 5(1): 73-77.
[18] Martin L, Latypova X, Terro F. Post-translational modifications of tau protein: implications for Alzheimer's disease [J]. Neurochem Int, 2011, 58(4): 458-471.
[19] Yuan H, Long H, Liu J, et al. Effects of infrasound on hippocampus-dependent learning and memory in rats and some underlying mechanisms [J]. Environ Toxicol Phar, 2009, 28(2): 243-247.
[20] Liu ZH, Chen JZ, Ye L, et al. Effects of infrasound at 8 Hz 90 dB/130 dB on NMDAR1 expression and changes in intracellular calcium ion concentration in the hippocampus of rats [J]. Mol Med Rep, 2010, 3(6): 917-921.
[21] 刘朝晖,陈景藻,李康樗,等. 不同声压级次声对大鼠海马细胞凋亡的影响[J]. 中华物理医学与康复杂志, 2004, 26(3): 148-151.
[22] 吕国蔚. 医学神经生物学[M]. 北京:高等教育出版社, 2000: 87-88, 184-202.
[23] Liang Z, Liu F, Iqbal K, et al. Decrease of protein phosphatase 2A and its association with accumulation and hyperphosphorylation of tau in Down syndrome [J]. J Alzheimers Dis, 2008, 13(3): 295-302.
[24] Avila J. Tau kinases and phosphatases: Commentary [J]. J Cell Mol Med, 2008, 12(1): 258-259.
[25] Wang YJ, Chen GH, Hu XY, et al. The expression of calcium/calmodulin-dependent protein kinase II-α in the hippocampus of patients with Alzheimer's disease and its links with AD-related pathology [J]. Brain Res, 2005, 1031(1): 101-108.

次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ及Tau蛋白表达的影响

Effects of Infrasound on Expression of Calmodulin-dependent Protein Kinase II and Tau Protein in Hippocampus of Rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	李芳, 霍速, 杜巨豹, 刘秀贞, 李小爽, 宋为群. 经颅直流电刺激联合任务导向性康复训练对脊髓损伤大鼠前肢运动障碍的效果[J]. 《中国康复理论与实践》, 2023, 29(7): 777-781.
[2]	罗兰, 李璐, 金沐. 氙气后处理对脊髓缺血再灌注损伤的效果：Akt信号通路和自噬机制[J]. 《中国康复理论与实践》, 2023, 29(2): 174-181.
[3]	黄志霖, 徐发邵, 施静, 黄淦, 刘梅芳, 张霞辉. 线栓法建立卒中后吞咽障碍的大鼠模型[J]. 《中国康复理论与实践》, 2023, 29(10): 1147-1153.
[4]	秦彦强, 董浩, 孙迎春, 程先宽, 姚海江. 不同针刺方案对卒中后抑郁大鼠神经递质及相关炎性因子的影响[J]. 《中国康复理论与实践》, 2023, 29(1): 30-37.
[5]	缪培,张通,米海霞,张伟东. 不同线栓法复制局灶性脑缺血模型大鼠恢复期学习记忆能力的差异及其机制[J]. 《中国康复理论与实践》, 2022, 28(7): 789-796.
[6]	周小珏,冯婧,庞日朝,刘捷,张安仁. 隔日限食减轻脊髓损伤大鼠炎症反应的芳香烃受体/细胞因子信号传导抑制因子2/核转录因子-κB信号通路机制[J]. 《中国康复理论与实践》, 2022, 28(5): 544-551.
[7]	宋绍霏,侯园园,王云雷,张通. 异常光周期所致昼夜节律紊乱对大鼠腓肠肌时钟基因和葡萄糖摄取相关基因表达节律的影响[J]. 《中国康复理论与实践》, 2022, 28(5): 552-558.
[8]	李童,方志鹏,邵玉萍,王平. 有氧运动对睡眠剥夺大鼠学习记忆及海马神经元突触可塑性的效果[J]. 《中国康复理论与实践》, 2022, 28(11): 1270-1277.
[9]	王静怡,尹杰,刘建成,庞日朝,王文春. 蜘蛛香环烯醚萜类成分对急性脊髓损伤大鼠神经细胞焦亡的影响[J]. 《中国康复理论与实践》, 2021, 27(6): 653-660.
[10]	周文美,陶陶,吴霜,王廷龙,杨正奕,张莹. 丰富环境对脑缺血再灌注损伤大鼠神经功能和缺血半暗带区葡萄糖代谢的影响[J]. 《中国康复理论与实践》, 2021, 27(5): 522-529.
[11]	王琼芬,王风波,王科,钟永强,王娇娇. 电针风池对急性脑梗死大鼠脑星形胶质细胞和神经元的保护效果[J]. 《中国康复理论与实践》, 2021, 27(3): 302-309.
[12]	李向哲,丁洁,王庆华,董传明,王彤,吴勤峰. 减重平板训练对脊髓损伤大鼠神经病理性疼痛及脊髓后角谷氨酸脱羧酶-65/67表达的影响[J]. 《中国康复理论与实践》, 2021, 27(2): 131-136.
[13]	艾坤,许明,刘琼,邓石峰,刘继生,祁芳,易细芹,瞿启睿,张泓. 电针对骶上脊髓损伤后逼尿肌亢进大鼠逼尿肌中环腺苷酸和蛋白激酶A含量及肌球蛋白轻链激酶磷酸化的影响[J]. 《中国康复理论与实践》, 2021, 27(2): 137-144.
[14]	凌梦钰,杨一卓,刘帅,叶超群. 运动训练对脑缺血再灌注大鼠认知功能及前额皮层神经元核抗原和突触素1表达的效果[J]. 《中国康复理论与实践》, 2021, 27(11): 1272-1281.
[15]	彭志锋, 张义平, 张继红. 早期运动联合戊四氮对脑缺血再灌注大鼠神经功能的效果及其机制[J]. 《中国康复理论与实践》, 2021, 27(1): 27-30.