针康法对脑缺血大鼠神经功能和缺血半暗区皮层血管内皮生长因子受体表达的影响

doi:10.3969/j.issn.1006-9771.2017.05.006

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (5): 520-524.doi: 10.3969/j.issn.1006-9771.2017.05.006

针康法对脑缺血大鼠神经功能和缺血半暗区皮层血管内皮生长因子受体表达的影响

叶涛¹, 唐强², 朱路文², 陈慧杰², 吴孝军¹, 李宏玉¹, 田源¹

1.黑龙江中医药大学,黑龙江哈尔滨市 150040;
2.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001。

收稿日期:2017-01-25 修回日期:2017-02-21 出版日期:2017-05-25 发布日期:2017-05-24
通讯作者: 唐强(1963-),男,汉族,四川大竹县人,博士,教授,主要研究方向：神经系统疾病中医康复基础与临床研究。E-mail: tangqiang1963@163.com。
作者简介:叶涛(1989-),男,汉族,福建漳平市人,博士研究生,主要研究方向：脑卒中中医康复基础与临床研究。
基金资助:
1.国家自然科学基金项目(No.81273818; No.81473762); 2.黑龙江中医药大学领军人才计划项目(No.2012RCL02)

Effects of Acupuncture-rehabilitation Therapy on Neurological Function and Expression of Vascular Endothelial Growth Factor Receptors, Flt-1 and Flk-1, after Focal Cerebral Ischemia in Rats

YE Tao¹, TANG Qiang², ZHU Lu-wen², CHEN Hui-jie², WU Xiao-jun¹, LI Hong-yu¹, TIAN Yuan¹

1. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China;
2. the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China

Received:2017-01-25 Revised:2017-02-21 Published:2017-05-25 Online:2017-05-24
Contact: TANG Qiang. E-mail: tangqiang1963@163.com

摘要/Abstract

摘要： 目的观察针康法对脑缺血大鼠神经功能和缺血半暗区皮层血管内皮生长因子受体Flt-1、Flk-1蛋白表达的影响。方法 90只雄性Sprague-Dawley大鼠随机分入假手术组、模型组、针刺组、康复组和针康组,每组再分为术后3 d、7 d、14 d三个亚组(n=6)。模型组、针刺组、康复组和针康组用线栓法制备永久性局灶性脑缺血模型。假手术组和模型组不进行治疗,针刺组采用头穴丛刺针法治疗,康复组给予跑台训练,针康组采用头穴丛刺结合跑台训练治疗。术后各时间点,采用改良神经损害严重程度评分(mNSS)进行评定,Western blotting法检测缺血半暗区皮层Flt-1、Flk-1表达。结果与模型组比较,各治疗组术后各时间点mNSS评分降低(P<0.05);术后7 d、14 d,与针刺组、康复组比较,针康组评分进一步降低(P<0.05)。与模型组比较,各治疗组术后各时间点Flt-1、Flk-1蛋白表达升高(P<0.05);与针刺组、康复组比较,针康组Flt-1、Flk-1蛋白表达水平进一步升高(P<0.05)。结论针康法治疗可进一步改善脑缺血大鼠神经行为,可能与其持续促进缺血半暗区皮层Flt-1、Flk-1蛋白表达有关。

关键词: 脑缺血, 针康法, 神经功能, 血管内皮生长因子受体, Flt-1, Flk-1, 大鼠

Abstract: Objective To explore the effect of acupuncture-rehabilitation therapy on neurological function and expression of Flt-1 and Flk-1, members of vascular endothelial growth factor receptors, after permanent focal cerebral ischemia in rats. Methods Ninety male Sprague-Dawley rats were divided into five groups, namely sham group, model group, acupuncture group, rehabilitation group and acupuncture-rehabilitation group, and each group was further divided into 3-day, 7-day and 14-day subgroups, equally. Their middle cerebral arteries were occluded except those of sham group. The sham and model groups accepted no treatment, while the acupuncture group accepted cluster needling of scalp acupuncture, the rehabilitation group accepted treadmill training, and the acupuncture-rehabilitation group accepted both acupuncture and treadmill training. They were assessed with modified Neurologic Severity Score (mNSS) 3, 7 and 14 days after modeling, while the expression of Flt-1 and Flk-1 were determined with Western blotting. Results The mNSS score reduced in all the treatment groups (P<0.05) compared with that of the model group at every time point, and was the least in the acupuncture-rehabilitation group (P<0.05) 7 and 14 days after modeling among the treatment groups. Meanwhile, the expression of Flt-1 and Flk-1 protein increased in all the treatment groups (P<0.05), and was the most in the acupuncture-rehabilitation group (P<0.05). Conclusion Acupuncture-rehabilitation therapy can promote the neurological function recovery in rat with permanent focal cerebral ischemia, which may be associated with the continuous inducement of Flt-1, Flk-1 protein expression in ischemic penumbra cortex.

Key words: cerebral ischemia, acupuncture-rehabilitation therapy, neurological function, vascular endothelial growth factor receptors, Flt-1, Flk-1, rats

中图分类号:

R743.3

叶涛, 唐强, 朱路文, 陈慧杰, 吴孝军, 李宏玉, 田源. 针康法对脑缺血大鼠神经功能和缺血半暗区皮层血管内皮生长因子受体表达的影响[J]. 《中国康复理论与实践》, 2017, 23(5): 520-524.

YE Tao, TANG Qiang, ZHU Lu-wen, CHEN Hui-jie, WU Xiao-jun, LI Hong-yu, TIAN Yuan. Effects of Acupuncture-rehabilitation Therapy on Neurological Function and Expression of Vascular Endothelial Growth Factor Receptors, Flt-1 and Flk-1, after Focal Cerebral Ischemia in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(5): 520-524.

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针康法对脑缺血大鼠神经功能和缺血半暗区皮层血管内皮生长因子受体表达的影响

Effects of Acupuncture-rehabilitation Therapy on Neurological Function and Expression of Vascular Endothelial Growth Factor Receptors, Flt-1 and Flk-1, after Focal Cerebral Ischemia in Rats

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