《中国康复理论与实践》

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (6): 628-633.doi: 10.3969/j.issn.1006-9771.2017.06.002

• 专题 脊髓损伤研究 • 上一篇    下一篇

电针对完全性脊髓损伤后神经源性膀胱大鼠脊髓组织中Caspase-9、细胞色素C及凋亡蛋白酶激活因子-1表达的影响

许明, 张泓, 刘继生, 尹秀婷, 张健, 黄桂兰, 艾坤   

  1. 湖南中医药大学,湖南长沙市 410208。
  • 收稿日期:2016-10-17 修回日期:2016-12-21 出版日期:2017-06-25 发布日期:2017-06-27
  • 通讯作者: 张泓(1963-),男,汉族,湖南常德市人,教授,博士研究生导师,主要研究方向：神经系统疾病的中西医结合康复机理与临床研究。E-mail: zh5381271@sina.com。
  • 作者简介:许明(1990-),男,汉族,湖南益阳市人,硕士研究生,主要研究方向：神经系统疾病的中西医结合康复机理与临床研究。
  • 基金资助:
    国家自然科学基金面上项目(No.81473753)

Effect of Electroacupuncture on Expression of Caspase-9, Cytochrome C and Apoptotic Protease Activating Factor 1 in Spinal Tissue in Rats with Neurogenic Bladder after Complete Spinal Cord Injury

XU Ming, ZHANG Hong, LIU Ji-sheng, YIN Xiu-ting, ZHANG Jian, HUANG Gui-lan, AI Kun   

  1. Hunan University of TCM, Changsha, Hunan 410208, China
  • Received:2016-10-17 Revised:2016-12-21 Published:2017-06-25 Online:2017-06-27
  • Contact: Correspondence to ZHANG Hong. E-mail: zh5381271@sina.com

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摘要： 目的 观察电针对完全性脊髓损伤后神经源性膀胱大鼠膀胱功能及脊髓组织中Caspase-9、细胞色素C (Cyt-C)及凋亡蛋白酶激活因子-1 (Apaf-1)表达的影响。方法 雌性Sprague-Dawley大鼠60只,随机抽取36只,采用改良T10脊髓横断法制作完全性脊髓损伤后神经源性膀胱模型,取24只成功模型分为模型组和电针组各12只,其余未造模大鼠24只随机分为假手术组和空白组各12只。于造模后第19天取次髎、中极、三阴交、大椎行电针,连续7 d后行尿流动力学检测,TUNEL法测定细胞凋亡率,Western blotting测定脊髓组织中Caspase-9、Cyt-C和Apaf-1蛋白的表达情况。结果 与空白组和假手术组比较,模型组和电针组膀胱最大容量及膀胱顺应性均明显降低(P<0.01),膀胱基础压力和漏尿点压力增加(P<0.05);脊髓组织TUNEL阳性率显著升高(P<0.001);脊髓组织中Caspase-9、Cyt-C和Apaf-1蛋白表达升高(P<0.05)。与模型组比较,电针组的膀胱最大容量及膀胱顺应性明显增加(P<0.01),膀胱基础压力和漏尿点压力降低(P<0.05);脊髓组织TUNEL阳性率明显降低(P<0.01);脊髓组织中Caspase-9、Cyt-C及Apaf-1蛋白表达量降低(P<0.05)。结论 电针次髎、中极、三阴交、大椎可改善完全性脊髓损伤后神经源性膀胱大鼠膀胱功能,其作用机制可能与脊髓组织中Caspase-9、Cyt-C和Apaf-1的表达下调,凋亡减少有关。

关键词: 脊髓损伤, 神经源性膀胱, 电针, Caspase-9, 细胞色素C, 凋亡蛋白酶激活因子-1, 凋亡, 大鼠

Abstract: Objective To observe the effect of electroacupuncture on Caspase-9, cytochrome C (Cyt-C) and apoptotic protease activating factor 1 (Apaf-1) protein in rats with neurogenic bladder after complete spinal cord injury. Methods A total of 60 female Sprague-Dawley rats were involved, in which 36 rats were randomly selected to establish the neurogenic bladder model with T10 spinal cord transected. 24 successful models selected were randomly divided into model group (n=12) and electroacupuncture group (n=12). The other 24 rats were also randomly divided into sham group (n=12) and blank group (n=12). The electroacupuncture group was electroacupunctured on Ciliao (BL32), Zhongji (RN3), Sanyinjiao (SP6) and Dazhui (GV14) for seven days. All of them were assessed with urodynamic test, TUNEL method was used to determine the apoptosis rate of the spinal cord, and Western blotting was used to detect the expression of Caspase-9, Cyt-C and Apaf-1 protein. Results Compared with the blank group and the sham group, the maximum bladder capacity and compliance decreased (P<0.01), the urinary bladder pressure and leakage point pressure increased (P<0.05); the apoptosis rate of the spinal cord increased (P<0.001); the expression of Caspase-9, Cyt-C and Apaf-1 protein increased (P<0.05) in the model group and the electroacupuncture group. Compared with the model group, the maximum bladder capacity and compliance increased (P<0.01), the urinary bladder pressure and leakage point pressure decreased (P<0.05); the apoptosis rate of the spinal cord decreased (P<0.05); the expression of Caspase-9, Cyt-C and Apaf-1 protein decreased (P<0.05) in the electroacupuncture group. Conclusion Electroacupuncture on Ciliao, Zhongji, Sanyinjiao and Dazhui could improve the bladder function after spinal cord injury in rats. The mechanisms may be related with the down-regulation of Caspase-9, Cyt-C and Apaf-1 protein to reduce apoptosis.

Key words: spinal cord injury, neurogenic bladder, electroacupuncture, Caspase-9, cytochrome C, apoptotic protease activating factor 1, apoptosis, rats

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