电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响

doi:10.3969/j.issn.1006-9771.2017.07.001

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (7): 745-749.doi: 10.3969/j.issn.1006-9771.2017.07.001

• 专题脑卒中针刺机理与功能康复 • 下一篇

电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响

叶涛¹, 朱路文², 唐强², 李宏玉¹, 梁碧莹¹, 张继瑶¹, 郑婷婷¹

1.黑龙江中医药大学,黑龙江哈尔滨市 150040;
2.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001。

收稿日期:2017-03-29 出版日期:2017-07-25 发布日期:2017-08-07
通讯作者: 朱路文(1983-),男,副教授,主要研究方向：神经系统疾病中医康复基础与临床研究。E-mail: zhuluwen1983@126.com。
作者简介:叶涛(1989-),男,汉族,福建漳平市人,博士研究生,主要研究方向：脑卒中中医康复基础与临床研究。
基金资助:
1.国家自然科学基金项目(No.81503666); 2.黑龙江省自然科学基金项目(No.QC2015103); 3.哈尔滨市科技创新人才研究专项基金项目(青年后备人)(No.2014RFQGJ150); 4.黑龙江中医药大学领军人才计划项目(No.2012RCL02); 5.黑龙江中医药大学研究生创新科研项目(No.yjscx2016037)

Effects of Electroacupuncture Pretreatment on Neurological Function, and Expression of Toll-like Receptor 4 and Nuclear Factor κB in Ischemic Penumbra in Rats after Cerebral Ischemia-reperfusion Injury

YE Tao¹, ZHU Lu-wen², TANG Qiang², LI Hong-yu¹, LIANG Bi-ying¹, ZHANG Ji-yao¹, ZHENG Ting-ting¹

1. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China;
2. The Second Hospital Affiliated to Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China

Received:2017-03-29 Published:2017-07-25 Online:2017-08-07
Contact: Correspondence to ZHU Lu-wen. E-mail: zhuluwen1983@126.com

摘要/Abstract

摘要： 目的探讨电针预处理对大鼠脑缺血再灌注损伤后神经功能的影响及其机制。方法雄性Sprague-Dawley大鼠36只随机分为假手术组(n=12)、模型组(n=12)和电针预处理组(n=12)。后两组线栓法制备大鼠脑缺血2 h再灌注模型。电针预处理组在缺血前给予电针百会干预2周。再灌注24 h后,采用改良神经功能缺损评分进行评定,HE染色观察缺血侧脑组织脑损伤程度,Western blotting检测脑缺血半暗区Tolls样受体4 (TLR4)、核转录因子κB (NF-κB)蛋白表达。结果与模型组比较,电针预处理组神经功能缺损评分降低(P<0.05),脑组织损伤程度减轻,缺血半暗区TLR4、NF-κB蛋白表达下调(P<0.05)。结论电针预处理通过下调缺血半暗区TLR4、NF-κB蛋白表达,诱导脑保护作用,降低炎性损伤程度,改善脑缺血再灌注损伤后大鼠的神经功能。

关键词: 脑缺血再灌注损伤, 电针, 预处理, 神经功能, Tolls样受体4, 核转录因子κ, B, 大鼠

Abstract: ObjectiveTo investigate the effect of electroacupuncture (EA) pretreatment on neurological function, and the expression of Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB) protein in ischemic penumbra after cerebral ischemia-reperfusion (CIR) injury. MethodsA total of 36 male Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and EA group (n=12). The later two groups were occluded their right middle cerebral arteries for two hours and reperfused. The EA group was pretreated with EA at Baihui (GV20) for two weeks before modeling. They were assessed with modified Neurological Severity Scores (mNSS), the injury in ischemic brain was detected with HE staining, and the expression of TLR4 and NF-κB protein in ischemic penumbra were detected with Western blotting, 24 hours after reperfusion. ResultsThe score of mNSS decreased (P<0.05), the injury of brain tissue ameliorated, and the expression of TLR4 and NF-κB decreased in ischemic penumbra (P<0.05) in EA group compared with those in the model group. ConclusionEA pretreatment can reduce the injury and improve the neurological function in rats after CIR by down-regulating the expression of TLR4 and NF-κB protein in ischemic penumbra.

Key words: cerebral ischemia-reperfusion injury, electroacupuncture, pretreatment, neurological function, Toll-like receptor 4, nuclear factor κ, B, rats

中图分类号:

R743.3

叶涛, 朱路文, 唐强, 李宏玉, 梁碧莹, 张继瑶, 郑婷婷. 电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响[J]. 《中国康复理论与实践》, 2017, 23(7): 745-749.

YE Tao, ZHU Lu-wen, TANG Qiang, LI Hong-yu, LIANG Bi-ying, ZHANG Ji-yao, ZHENG Ting-ting. Effects of Electroacupuncture Pretreatment on Neurological Function, and Expression of Toll-like Receptor 4 and Nuclear Factor κB in Ischemic Penumbra in Rats after Cerebral Ischemia-reperfusion Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(7): 745-749.

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电针预处理对脑缺血再灌注损伤大鼠神经功能和缺血半暗区Tolls样受体4、核转录因子κB蛋白的影响

Effects of Electroacupuncture Pretreatment on Neurological Function, and Expression of Toll-like Receptor 4 and Nuclear Factor κB in Ischemic Penumbra in Rats after Cerebral Ischemia-reperfusion Injury

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