电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响

doi:10.3969/j.issn.1006-9771.2017.07.002

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (7): 750-755.doi: 10.3969/j.issn.1006-9771.2017.07.002

• 专题脑卒中针刺机理与功能康复 • 上一篇下一篇

电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响

宋长明^{1, 2, 3}, 黄佳^{1, 2, 3}, 林冰冰^{1, 2, 3}, 杨敏光^{1, 2, 3}, 张秀峰^{1, 2, 3}, 柳维林^{1, 2, 3}, 陶静^{1, 2, 3}, 陈立典^{1, 2, 3, 4}

1.福建中医药大学康复医学院,福建福州市 350122;
2.福建省康复技术重点实验室,福建福州市 350122;
3.福建康复产业研究院技术创新平台,福建福州市 350122;
4.国家中医药管理局中医康复研究中心,福建福州市 350122。

收稿日期:2017-04-03 出版日期:2017-07-25 发布日期:2017-08-07
通讯作者: 陈立典(1963-),男,福建政和县人,博士,教授,主任医师,博士生导师,主要研究方向：神经康复及认知科学。E-mail: fjtcm1958@sina.com。
作者简介:宋长明(1990-),女,汉族,河南安阳市人,硕士研究生,主要研究方向：神经康复及认知科学。
基金资助:
1.国家自然科学基金项目(No.81403462); 2.福建省教育厅省属高校项目(No.JK2014022)

Effects of Electroacupuncture at Baihui and Shenting on Learning-memory Function and Ultrastructure in Hippocampal CA1 in Cerebral Ischmeia-reperfusion Rats

SONG Chang-ming^{1, 2, 3}, HUANG Jia^{1, 2, 3}, LIN Bing-bing^{1, 2, 3}, YANG Min-guang^{1, 2, 3}, ZHANG Xiu-feng^{1, 2, 3}, LIU Wei-lin^{1, 2, 3}, TAO Jing^{1, 2, 3}, CHEN Li-dian^{1, 2, 3, 4}

1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China;
2. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350122, China;
3. Technology Innovation Platform of Fujian Rehabilitation Industry Research Institute, Fuzhou, Fujian 350122, China;
4. Rehabilitation Research Center of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China

Received:2017-04-03 Published:2017-07-25 Online:2017-08-07
Contact: Correspondence to CHEN Li-dian. E-mail: fjtcm1958@sina.com

摘要/Abstract

摘要： 目的观察电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及损伤大鼠海马CA1区突触超微结构的影响。方法雄性Sprague-Dawley大鼠25只随机分为假手术组(n=6)和手术组(n=19)。手术组线栓法建立左侧大脑中动脉栓塞90 min后再灌注模型,筛选符合纳入标准的12只大鼠随机分为模型组和电针组各6只。电针组电针百会、神庭穴7 d。采用Longa评分检测大鼠神经功能;小动物磁共振成像分析系统T₂加权成像观察脑梗死体积;Barnes迷宫测试检测大鼠学习记忆能力;透射电镜观察海马CA1区突触超微结构。结果与模型组相比,电针组Longa评分降低(P<0.05),脑梗死体积明显减少(P<0.01);Barnes迷宫测试逃避潜伏期明显缩短(P<0.01),平均进入错误洞口次数显著减少(P<0.001)。透射电镜显示,模型组突触结构溶解,数量减少,囊泡稀疏、破坏;与模型组相比,电针组突触结构较清晰,突触数量较多,突触囊泡较多。结论电针百会、神庭能有效改善脑缺血再灌注大鼠的学习记忆能力,其机制可能与改善海马CA1区突触的超微结构,提高突触可塑性有关。

关键词: 脑缺血再灌注, 电针, 突触, 超微结构, 学习记忆, 海马, 大鼠

Abstract: ObjectiveTo observe the effect of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning-memory function and ultrastructure in hippocampal CA1 region of rats after cerebral ischmeia-reperfusion. MethodsA total of 25 male Sprague-Dawley rats were randomly divided into sham group (n=6) and operation group (n=19). The operation group was occluded the left middle cerebral arteries with modified Longa's Methods for 90 minutes and reperfused, and twelve qualified rats of them were randomly divided into model group (n=6) and electroacupuncture group (n=6), the later accepted electroacupuncture at Baihui and Shenting for seven days. They were assessed with Longa's scores, and tested with Barnes maze. Their cerebral infarct volume was tested with magnetic resonance imaging T₂-weighted image. The ultrastructure of synapse in hippocampal CA1 region was observed with transmission electron microscope. ResultsCompared with the model group, the Longa's score improved (P<0.05), the infarct volume decreased (P<0.01), the average escape latency decreased (P<0.01) and the times entering the wrong hole decreased (P<0.001) in the electroacupuncture group. Under the transmission electron microscope, the number of synapse decreased in the model group, with the structure damage and vesicles sparse; compared with the model group, the number of synapse increased in the electroacupuncture group, with clear and complete structure and rich vesicles. ConclusionElectroacupuncture at Baihui and Shenting can improve the learning-memory function in rats after cerebral ischmeia-reperfusion, which may relate to improvement of synaptic plasticity and ameliorating ultrastructure in hippocampal CA1 region.

Key words: cerebral ischmeia-reperfusion, electroacupuncture, synapse, ultrastructure, learning-memory, hippocampus, rats

中图分类号:

R743.3

宋长明, 黄佳, 林冰冰, 杨敏光, 张秀峰, 柳维林, 陶静, 陈立典. 电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响[J]. 《中国康复理论与实践》, 2017, 23(7): 750-755.

SONG Chang-ming, HUANG Jia, LIN Bing-bing, YANG Min-guang, ZHANG Xiu-feng, LIU Wei-lin, TAO Jing, CHEN Li-dian. Effects of Electroacupuncture at Baihui and Shenting on Learning-memory Function and Ultrastructure in Hippocampal CA1 in Cerebral Ischmeia-reperfusion Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(7): 750-755.

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电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响

Effects of Electroacupuncture at Baihui and Shenting on Learning-memory Function and Ultrastructure in Hippocampal CA1 in Cerebral Ischmeia-reperfusion Rats

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