大鼠脊髓损伤后ski相关蛋白表达的时空变化规律及作用

doi:10.3969/j.issn.1006-9771.2017.08.009

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (8): 912-918.doi: 10.3969/j.issn.1006-9771.2017.08.009

大鼠脊髓损伤后ski相关蛋白表达的时空变化规律及作用

朱彦东^1,2，周开升^1,2，郭永强^1,2，江龙^1,2，郑利强^1,2，汪静²，李森³，龙在云³，伍亚民³，张海鸿¹

1.兰州大学第二医院骨科，甘肃兰州市 730030；
2.甘肃省骨关节疾病研究重点实验室，甘肃兰州市 730030；
3.第三军医大学大坪医院野战外科研究所三室，创伤、烧伤与复合伤国家重点实验室，重庆市 400042。

收稿日期:2017-03-06 修回日期:2017-04-11 出版日期:2017-08-25 发布日期:2017-08-24
通讯作者: 张海鸿，主任医师，副教授，硕士研究生导师，主要研究方向：脊柱外科。E-mail: zhanghaihong1968@sina.com。
作者简介:朱彦东(1989-)，男，汉族，甘肃天水市人，硕士研究生，主要研究方向：脊柱脊髓损伤。
基金资助:
国家自然科学基金项目(No.30772299)。

Temporal and Spatial Variation of ski-interacting Protein Expression in Rats after Spinal Cord Injury and its Role　

ZHU Yan-dong^1,2, ZHOU Kai-sheng^1,2, GUO Yong-qiang^1,2, JIANG Long^1,2, ZHENG Li-qiang^1,2, WANG Jing^1,2, LI Sen³, LONG Zai-yun³, WU Ya-min³, ZHANG Hai-hong¹

1. Department of Orthopedics, Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, China;
2. Key Laboratory of Orthopedics of Gansu Province, Lanzhou, Gansu 730030, China;
3. State Key Laboratory of Trauma, Burns and Combined Injury, the Third Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China

Received:2017-03-06 Revised:2017-04-11 Published:2017-08-25 Online:2017-08-24
Contact: ZHANG Hai-hong. E-mail: zhanghaihong1968@sina.com

摘要/Abstract

摘要： 目的探究大鼠脊髓损伤后ski相关蛋白(SKIP)的表达及其变化规律。方法 60只成年雌性Sprague-Dawley大鼠随机分为假手术组(n=30)和打击组(n=30)，每组分为1 d、3 d、5 d、7 d、14 d五个时间点，各6只。打击组用Allen法制作T10打击损伤模型，假手术组只咬开椎板。采用BBB评分评价后肢运动功能；尼氏染色观察损伤后脊髓神经元的病理变化；免疫荧光染色观察损伤脊髓SKIP的表达情况。结果打击组各时间点BBB评分均显著低于假手术组(t>48.267, P<0.001)。与假手术组相比，打击组5 d脊髓神经元胞浆中尼氏小体开始崩解、凝聚，分布不规则，神经元变性坏死，14 d时损伤加重。免疫荧光染色显示，SKIP主要在脊髓灰质中表达，白质中极少表达；损伤后SKIP表达逐渐上升，5 d时达到高峰(t=-17.035, P<0.001)，14 d时明显降低(t=3.853, P<0.05)。结论 SKIP可能是一种作用于神经元并影响其凋亡的新的信号分子。

关键词: 脊髓损伤, ski相关蛋白, 神经元, 凋亡, 大鼠

Abstract: Objective To explore the expression and change of ski-interacting protein (SKIP) in rats after spinal cord injury. Methods A total of 60 adult female Sprague-Dawley rats were randomly divided into sham group (n=30) and spinal cord injury (SCI) group (n=30), each group was further divided into five time points including one day, three days, five days, seven days, and 14 days with six rats in each time points. The model was established at T10 with modified Allen's technique, and the sham group only bit the lamina of rats. The hindlimbs behavior was assessed with Basso-Beattie-Bresnahan (BBB) score at each time point. The pathological changes of spinal cord neurons were detected with Nissl staining. The expression of SKIP were observed with immunofluorescence staining. Results The BBB scores were significantly lower in each time point in SCI group than in the sham group (t>48.267, P<0.001). Compared with the sham group, Nissl bodies in the cytoplasm of spinal cord neurons began to disintegrate, coalesce and irregularly distribute, the neurons began to degenerate and die on the fifth day, and the damage deteriorated on the 14th day. Immunofluorescence staining showed that SKIP expression was mainly expressed in the gray matter of the spinal cord and little expressed in the white matter. The expression of SKIP gradually increased after SCI, and reached a peak on the fifth day (t=-17.035, P<0.001) and decreased significantly on the 14th day (t=3.853, P<0.05). Conclusion SKIP may be a new signaling molecule, which play an important role in neuronal apoptosis after SCI.

Key words: spinal cord injury, ski-interacting protein, neuron, apoptosis, rats

中图分类号:

R651.2

朱彦东,周开升,郭永强,江龙,郑利强,汪静，李森，龙在云，伍亚民，张海鸿. 大鼠脊髓损伤后ski相关蛋白表达的时空变化规律及作用[J]. 《中国康复理论与实践》, 2017, 23(8): 912-918.

ZHU Yan-dong,ZHOU Kai-sheng,GUO Yong-qiang,JIANG Long,ZHENG Li-qiang,WANG Jing,LI Sen, LONG Zai-yun, WU Ya-min, ZHANG Hai-hong. Temporal and Spatial Variation of ski-interacting Protein Expression in Rats after Spinal Cord Injury and its Role　[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(8): 912-918.

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大鼠脊髓损伤后ski相关蛋白表达的时空变化规律及作用

Temporal and Spatial Variation of ski-interacting Protein Expression in Rats after Spinal Cord Injury and its Role

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