APP/PS1转基因小鼠学习记忆能力下降与胼胝体脱髓鞘损伤的关系

doi:10.3969/j.issn.1006-9771.2017.09.008

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (9): 1027-1031.doi: 10.3969/j.issn.1006-9771.2017.09.008

APP/PS1转基因小鼠学习记忆能力下降与胼胝体脱髓鞘损伤的关系

张秀峰¹, 金昊¹, 林冰冰¹, 李珑¹, 宋长明¹, 李钻芳², 梁胜祥³, 毛敬洁², 柳维林¹, 陶静¹, 陈立典^{4, 5, 6}

1.福建中医药大学康复医学院,福建福州市 350122；
2.福建中医药大学中西医结合研究院,福建福州市 350122；
3.郑州大学物理工程学院,河南郑州市 450001；
4.国家中医药管理局中医康复研究中心,福建福州市 350122；
5.福建省康复技术协同创新中心,福建福州市 350122；
6.康复医疗技术国家地方联合工程研究中心,福建福州市 350122。

收稿日期:2017-05-08 修回日期:2017-06-22 出版日期:2017-09-25 发布日期:2017-10-10
通讯作者: 陈立典(1963-),男,福建政和市人,教授,主任医师,博士生导师,主要研究方向：神经康复及认知科学。E-mail: cld@fjtcm.edu.cn。
作者简介:张秀峰(1986-),女,汉族,江苏泰州市人,硕士研究生,主要研究方向：神经康复与认知科学研究。
基金资助:
1.福建省康复技术协同创新中心课题(No.X2016001-协同); 2.福建中医药大学科研创新平台专项(No.X2015024-平台)

Learning and Memory Deficit and Demyelination of Corpus Callosum in APP/PS1 Transgenic Mice

ZHANG Xiu-feng¹, JIN Hao¹, LIN Bing-bing¹, LI Long¹, SONG Chang-ming¹, LI Zuan-fang², LIANG Sheng-xiang³, MAO Jing-jie², LIU Wei-lin¹, TAO Jing¹, CHEN Li-dian^{4, 5, 6}

1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China;
2. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fujian 350122, China;
3. School of Physics and Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China;
4. Rehabilitation Research Center of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China;
5. Fujian Collaborative Innovation Center for Rehabilitation Technology, Fuzhou, Fujian 350122, China;
6. Rehabilitation Medical Technology Joint National Local Engineering Research Center, Fuzhou, Fujian 350122, China

Received:2017-05-08 Revised:2017-06-22 Published:2017-09-25 Online:2017-10-10
Contact: Correspondence to CHEN Li-dian. E-mail: cld@fjtcm.edu.cn

摘要/Abstract

摘要： 目的探讨12月龄APP/PS1转基因小鼠学习记忆能力下降与胼胝体脱髓鞘损伤之间的关系。方法12只12月龄APP/PS1转基因小鼠为AD组,12只同窝阴性小鼠为野生组。Morris水迷宫检测两组学习记忆能力;卢卡斯快蓝染色观察胼胝体神经纤维形态;免疫组化检测胼胝体髓鞘碱性蛋白(MBP)含量;硫黄素S染色观察淀粉样斑块在胼胝体的沉积情况。结果与野生组相比,AD组逃避潜伏期明显延长(Z>2.873, P<0.01),穿越平台次数显著减少(t=-7.339, P<0.001)。AD组胼胝体神经纤维髓鞘稀疏,排列紊乱,出现较多空洞;MBP含量较野生组显著降低(t=-4.481, P<0.001);胼胝体区出现淀粉样斑块沉积。结论12月龄APP/PS1转基因小鼠学习记忆能力下降,可能与淀粉样斑块在胼胝体的沉积引起胼胝体神经纤维脱髓鞘损伤有关。

关键词: 阿尔茨海默病, 学习记忆, 胼胝体, 脱髓鞘, 小鼠

Abstract: ObjectiveTo investigate the relationship between learning and memory deficit and demyelination of the corpus callosum in twelve-month old APP/PS1 transgenic mice. MethodsTwelve twelve-month old APP/PS1 transgenic mice were as AD group, and age-matched wild type (WT) littermates were as WT group. Learning and memory ability was tested with Morris water maze, and the morphology of nerve fiber of corpus callosum was detected with Luxol Fast Blue staining. Immunohistochemistry was used to detect myelin basic protein (MBP) in the corpus callosum. Thioflavine S staining was used to detect amyloid plaque in the corpus callosum. ResultsCompared with WT group, the latency increased (Z>2.873, P<0.01) and the times crossing the location of the platform decreased (t=-7.339, P<0.001) in AD group. The nerve fibers were sparse and disorganized, with a lot of vacuoles in the corpus callosum of AD group. The positive expression of MBP in the corpus callosum was significantly decreased (t=-4.481, P<0.001) in AD group compared with WT group. There were amyloid plaques in the corpus callosum of AD group. ConclusionTwelve-month old APP/PS1 transgenic mice exhibit learning and memory deficit, which may be attributed to the deposition of the amyloid plaque mediated demyelinated injury of the corpus callosum.

Key words: Alzheimer's disease, learning and memory, corpus callosum, demyelination, mice

中图分类号:

R749.1

张秀峰, 金昊, 林冰冰, 李珑, 宋长明, 李钻芳, 梁胜祥, 毛敬洁, 柳维林, 陶静, 陈立典. APP/PS1转基因小鼠学习记忆能力下降与胼胝体脱髓鞘损伤的关系[J]. 《中国康复理论与实践》, 2017, 23(9): 1027-1031.

ZHANG Xiu-feng, JIN Hao, LIN Bing-bing, LI Long, SONG Chang-ming, LI Zuan-fang, LIANG Sheng-xiang, MAO Jing-jie, LIU Wei-lin, TAO Jing, CHEN Li-dian. Learning and Memory Deficit and Demyelination of Corpus Callosum in APP/PS1 Transgenic Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(9): 1027-1031.

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APP/PS1转基因小鼠学习记忆能力下降与胼胝体脱髓鞘损伤的关系

Learning and Memory Deficit and Demyelination of Corpus Callosum in APP/PS1 Transgenic Mice

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