迷走神经电刺激对脑外伤昏迷大鼠意识及前额叶皮质γ-氨基丁酸b1受体表达的影响

doi:10.3969/j.issn.1006-9771.2017.09.010

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (9): 1037-1042.doi: 10.3969/j.issn.1006-9771.2017.09.010

迷走神经电刺激对脑外伤昏迷大鼠意识及前额叶皮质γ-氨基丁酸b1受体表达的影响

廖诚诚, 冯珍, 黄菲菲, 陈琴

南昌大学第一附属医院康复医学科,江西南昌市 330006。

收稿日期:2016-11-05 修回日期:2017-05-11 出版日期:2017-09-25 发布日期:2017-10-10
通讯作者: 冯珍,女,江西南昌市人,教授,主任医师,主要研究方向：神经康复。E-mail: fengzhenly@sina.com。
作者简介:廖诚诚(1988-),女,汉族,江西赣州市人,硕士研究生,主要研究方向：神经康复。
基金资助:
国家自然科学基金项目(No.81260295)

Effect of Vagus Nerve Stimulation on Wake-promoting and Expression of γ-aminobutyric Acid b1 Receptor in Prefrontal Cortex of Coma Rats post Traumatic Brain Injury

LIAO Cheng-cheng, FENG Zhen, HUANG Fei-fei, CHEN Qin

Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China

Received:2016-11-05 Revised:2017-05-11 Published:2017-09-25 Online:2017-10-10
Contact: Correspondence to FENG Zhen. E-mail: fengzhenly@sina.com

摘要/Abstract

摘要： 目的研究迷走神经电刺激(VNS)对脑外伤(TBI)昏迷大鼠的促醒效果及其机制。方法168只健康Sprague-Dawley大鼠随机分为空白组、TBI组、拮抗剂组和VNS组,每组42只。空白组不做任何处理,TBI组、VNS组、拮抗剂组撞击法复制脑外伤模型,造模后昏迷至少30 min的大鼠入选。VNS组予VNS,拮抗剂组侧脑室注射Orexin A受体1拮抗剂SB334867后加VNS,TBI组予假VNS。分别于干预后6 h、12 h、24 h观察大鼠意识水平,应用免疫组化、Western blotting检测前额叶皮质γ-氨基丁酸b1受体(GABAb1R)表达水平。结果最终空白组42只、TBI组11只、拮抗剂组13只、VNS组28只大鼠觉醒。Western blotting显示,干预后12 h、24 h,GABAb1R蛋白表达TBI组>拮抗剂组>空白组>VNS组(F>60.412, P<0.001)。免疫组化显示,GABAb1R蛋白表达TBI组>拮抗剂组>VNS组>空白组(H=15.121, P=0.002),但不同时间点无显著性差异(H=3.028, P=0.220)。结论VNS可促TBI昏迷大鼠觉醒,其促醒机制可能与Orexin A介导大鼠前额叶皮质GABAb1R表达水平下调有关。

关键词: 脑外伤, 昏迷, 迷走神经电刺激, 促醒, γ, -氨基丁酸b1受体, 大鼠

Abstract: ObjectiveTo investigate the wake-promoting effect of vagus nerve stimulation (VNS) on coma rats after traumatic brain injury (TBI), and the related mechanism. MethodsA total of 168 healthy Sprague-Dawley rats were randomly divided into blank group, TBI group, antagonist group and VNS group, 42 rats in each group. The latter three groups were established TBI model with impact, and the rats in coma at least 30 minutes were included. VNS group accepted VNS, the antagonist group were injected intralateroventricularly Orexin A receptor 1 (OXR1) antagonist SB334867, and TBI group accepted sham VNS. Their behaviors were observed to determine the level of consciousness six, twelve and 24 hours after intervention, while the expression of γ-aminobutyric acid b1 receptor (GABAb1R) in prefrontal cortex was detected with immunohistochemistry and Western blotting. ResultsThere were 42 rats in the blank group, 11 rats in TBI group, 13 rats in the antagonist group, and 28 rats in VNS group awakened finally. The expression of GABAb1R in prefrontal cortex ranged as TBI group, antagonist group, blank group and VNS group from more to less twelve and 24 hours after intervention under Western blotting (F>60.412, P<0.001), and it ranged as TBI group, antagonist group, VNS group and blank group under immunohistochemistry (H=15.121, P=0.002), with no significant difference among time points (H=3.028, P=0.220). ConclusionVNS can promote waking from coma in rats after TBI, which may relate with the decrease of GABAb1R in prefrontal cortex that induced by Orexin A.

Key words: traumatic brain injury, coma, vagus nerve stimulation, wake-promoting, γ, -aminobutyric acid b1 receptor, rats

中图分类号:

R651.1

廖诚诚, 冯珍, 黄菲菲, 陈琴. 迷走神经电刺激对脑外伤昏迷大鼠意识及前额叶皮质γ-氨基丁酸b1受体表达的影响[J]. 《中国康复理论与实践》, 2017, 23(9): 1037-1042.

LIAO Cheng-cheng, FENG Zhen, HUANG Fei-fei, CHEN Qin. Effect of Vagus Nerve Stimulation on Wake-promoting and Expression of γ-aminobutyric Acid b1 Receptor in Prefrontal Cortex of Coma Rats post Traumatic Brain Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(9): 1037-1042.

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迷走神经电刺激对脑外伤昏迷大鼠意识及前额叶皮质γ-氨基丁酸b1受体表达的影响

Effect of Vagus Nerve Stimulation on Wake-promoting and Expression of γ-aminobutyric Acid b1 Receptor in Prefrontal Cortex of Coma Rats post Traumatic Brain Injury

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