电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响

doi:10.3969/j.issn.1006-9771.2018.01.008

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (1): 43-48.doi: 10.3969/j.issn.1006-9771.2018.01.008

电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响

黄佳¹, 宋长明¹, 杨敏光¹, 李建鸿¹, 黄盛¹, 柳维林¹, 陶静^1,2,3, 陈立典^1,2,3,4

1.福建中医药大学康复医学院,福建福州市 350122
2.福建省康复技术协同创新中心,福建福州市 350122
3.康复医疗技术国家地方联合工程研究中心,福建福州市 350122
4.国家中医药管理局中医康复研究中心,福建福州市 350122

收稿日期:2017-09-17 修回日期:2017-11-09 出版日期:2018-01-25 发布日期:2018-01-31
通讯作者: 陈立典(1963-),男,福建政和县人,博士,教授,主任医师,博士生导师,主要研究方向：神经康复及认知科学;E-mail:fjtcm1958@sina.com。
作者简介:黄佳(1984-),女,汉族,重庆市人,博士研究生,主要研究方向：神经康复及认知科学。
基金资助:
1.国家自然科学基金项目(No.81403462); 2.福建省卫生厅青年科研项目(No.2014-1-70)

Effects of Electroacupuncture at Baihui and Shenting on Learning and Memory and Expression of Purinoceptor P2X7 in Hippocampal CA1 after Cerebral Ischmeia-reperfusion in Rats

HUANG Jia¹, SONG Chang-ming¹, YANG Min-guang¹, LI Jian-hong¹, HUANG Sheng¹, LIU Wei-lin¹, TAO Jing^1,2,3, CHEN Li-dian^1,2,3,4

1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
2. Fujian Collaborative Innovation Center for Rehabilitation Technology, Fuzhou, Fujian 350122, China
3. National & Local Joint Engineering Research Center of Rehabilitation Medical Technology, Fuzhou, Fujian 350122, China;
4. Rehabilitation Research Center of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China

Received:2017-09-17 Revised:2017-11-09 Published:2018-01-25 Online:2018-01-31
Contact: CHEN Li-dian.E-mail:fjtcm1958@sina.com
Supported by:
National Natural Science Foundation of China (No. 81403462) and Youth Research Project of Fujian Health Department (No. 2014-1-70)

摘要/Abstract

摘要： 目的观察电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆功能的影响,并探究其可能机制。方法雄性Sprague-Dawley大鼠42只随机分为假手术组(n=12)和手术组(n=30)。手术组线栓法制备左侧大脑中动脉栓塞90 min再灌注模型,符合纳入标准的24只分为模型组(n=12)和电针组(n=12)。电针组电针百会、神庭共7 d。造模后2 h和干预后1 d、3 d、7 d,采用Longa神经行为学评分进行评定;干预后3 d起行Barnes迷宫实验检测,共5 d;干预7 d后,免疫荧光标记法检测缺血侧海马CA1区嘌呤受体P2X7的表达,酶联免疫吸附法检测海马CA1区白细胞介素-1β (IL-1β)、肿瘤坏死因子-α (TNF-α)。结果干预后7 d,与模型组相比,电针组Longa评分降低(P<0.05);与假手术组相比,模型组Barnes迷宫逃避潜伏期显著延长(P<0.001),进入错误洞口次数显著增多(P<0.001);与模型组相比,电针组逃避潜伏期显著缩短(P<0.001),进入错误洞口次数显著减少(P<0.001);与假手术组比较,模型组P2X7受体平均光密度,IL-1β、TNF-α水平均显著提高(P<0.001);与模型组相比,电针组P2X7受体表达,IL-1β、TNF-α水平减少(P<0.05)。结论电针百会、神庭穴能有效改善缺血再灌注损伤大鼠的学习记忆能力,可能与抑制海马CA1区嘌呤受体P2X7表达,改善缺血再灌注损伤大鼠海马CA1区神经炎症反应有关。

关键词: 脑缺血再灌注, 电针, 嘌呤受体, P2X7, 海马, 学习记忆, 炎症, 大鼠

Abstract: Objective To observe the effect of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning and memory in rats after cerebral ischmeia-reperfusion and the possible mechanism.Methods A total of 42 male Sprague-Dawley rats were randomly divided into sham group (n=12) and operation group (n=30). The left middle cerebral arteries of the operation group were occluded with the modified Longa's method for 90 minutes and reperfused, and 24 qualified rats were randomly divided into model group (n=12) and electroacupuncture group (n=12), and the latter accepted electroacupuncture at Baihui and Shenting for seven days. They were assessed with Longa's score two hours after modeling, and one, three, seven days after intervention. They were tested with Barnes maze since three days after intervention, once a day for five days. The expression of purinoceptor P2X7 in CA1 of the hippocampus were detected with immunofluorescence seven days after intervention, while the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in CA1 were detected with enzyme-linked immunosorbent assay.Results The Longa's score was improved in the electroacupuncture group compared with that in the model group seven days after intervention (P<0.05); while the escape latency and the times entering the wrong hole increased in the model group compared with that in the sham group (P<0.001), and decreased in the electroacupuncture group compared with that in the model group (P<0.001). The expression of P2X7, IL-1β and TNF-α increased in the model group compared with the sham operation group (P<0.001), and decreased in the electroacupuncture group compared with that in the model group (P<0.05).Conclusion Electroacupuncture at Baihui and Shenting can improve the learning and memory in rats after cerebral ischemia-reperfusion, which may associate with inhibition of P2X7 to alleviate inflammation in hippocampus.

Key words: cerebral ischemia-reperfusion, electroacupuncture, purinoceptor, P2X7, hippocampus, learning and memory, inflammation, rats

中图分类号:

R743.3

黄佳, 宋长明, 杨敏光, 李建鸿, 黄盛, 柳维林, 陶静, 陈立典. 电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响[J]. 《中国康复理论与实践》, 2018, 24(1): 43-48.

HUANG Jia, SONG Chang-ming, YANG Min-guang, LI Jian-hong, HUANG Sheng, LIU Wei-lin, TAO Jing, CHEN Li-dian. Effects of Electroacupuncture at Baihui and Shenting on Learning and Memory and Expression of Purinoceptor P2X7 in Hippocampal CA1 after Cerebral Ischmeia-reperfusion in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 43-48.

参考文献

[1] Mozaffarian D, Benjamin EJ, Go AS, et al. Executive Summary: Heart Disease and Stroke Statistics-2016 Update: a report from the American Heart Association [J]. Circulation, 2016, 133(4): 447-454.
[2] Murphy SL, Xu J, Kochanek KD, et al. Deaths: Final Data for 2013 [J]. Natl Vital Stat Rep, 2016, 64(2): 1-119.
[3] Agnès J, Christine B, Olivier R, et al. Post-stroke cognitive impairment: high prevalence and determining factors in a cohort of mild stroke [J]. J Alzheimers Dis, 2014, 40(4): 1029-1038.
[4] Taylor RA, Sansing LH. Microglial responses after ischemic stroke and intracerebral hemorrhage [J]. Clin Dev Immunol, 2013, 2013: 746068.
[5] Feng X, Yang S, Liu J, et al. Electro-acupuncture ameliorates cognitive impairment through inhibition of NF-κB-mediated neuronal cell apoptosis in cerebral ischemia-reperfusion injured rats [J]. Mol Med Rep, 2013, 7(5): 1516-1522.
[6] 宋长明,黄佳,林冰冰,等. 电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响[J]. 中国康复理论与实践, 2017, 23(7): 750-755.
[7] 林志诚,杨珊莉,薛偕华,等. 针刺百会穴改善脑卒中患者记忆力的中枢机制[J]. 中国康复理论与实践, 2015, 20(2): 184-188.
[8] Jiang C, Yang S, Tao J, et al. Clinical Efficacy of acupuncture treatment in combination with RehaCom Cognitive Training for improving cognitive function in stroke: a 2×2 factorial design randomized controlled trial [J]. J Am Med Dir Assoc, 2016, 17(12): 1114-1122.
[9] Tort ABL, Komorowski RW, Manns JR, et al. Theta-gamma coupling increases during the learning of item-context associations [J]. Proc Natl Acad Sci USA, 2009, 106(49): 20942-20947.
[10] Nikonenko AG, Radenovic L, Andjus PR, et al. Structural features of ischemic damage in the hippocampus [J]. Anat Rec, 2009, 292(12): 1914-1921.
[11] Kerchner GA, Hess CP, Hammondrosenbluth KE, et al. Hippocampal CA1 apical neuropil atrophy in mild Alzheimer disease visualized with 7-T MRI (Podcast) [J]. Neurology, 2010, 75(15): 1381-1387.
[12] Kerchner GA, Deutsch GK, Zeineh M, et al. Hippocampal CA1 apical neuropil atrophy and memory performance in Alzheimer's disease [J]. Neuroimage, 2012, 63(1): 194-202.
[13] Pankratov YV, Lalo UV, Krishtal OA. Role for P2X receptors in long-term potentiation [J]. J Neurosci, 2002, 22(19): 8363-8369.
[14] Chin Y, Kishi M, Sekino M, et al. Involvement of glial P2Y1 receptors in cognitive deficit after focal cerebral stroke in a rodent model [J]. J Neuroinflammation, 2013, 10(1): 95.
[15] Illes P, Verkhratsky A. Purinergic neurone-glia signalling in cognitive-related pathologies [J]. Neuropharmacology, 2016, 104: 62-75.
[16] Amantea D, Micieli G, Tassorelli C, et al. Rational modulation of the innate immune system for neuroprotection in ischemic stroke [J]. Front Neurosci, 2015, 9(12): 2366-2378.
[17] Burnstock G. P2X ion channel receptors and inflammation [J]. Purinergic Signal, 2016, 12(1): 59-67.
[18] Deng Y, Guo XL, Yuan X, et al. P2X7 receptor antagonism attenuates the intermittent hypoxia-induced spatial deficits in a murine model of sleep apnea via inhibiting neuroinflammation and oxidative stress [J]. Chin Med J (Engl), 2015, 128(16): 2168-2175.
[19] Chu K, Yin B, Wang J, et al. Inhibition of P2X7 receptor ameliorates transient global cerebral ischemia/reperfusion injury via modulating inflammatory responses in the rat hippocampus [J]. J Neuroinflammation, 2012, 9(1): 150-159.
[20] Joana A, Alberto PS, Miroslav G, et al. P2X7 receptor blockade prevents ATP excitotoxicity in neurons and reduces brain damage after ischemia [J]. Neurobiol Dis, 2012, 45(3): 954-961.
[21] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989, 20(1): 84-91.
[22] 李忠仁. 实验针灸学[M]. 北京:中国中医药出版社, 2003.
[23] 李梦瑶,李向荣,高琼珏,等. 电针对局灶性脑缺血再灌注大鼠血中EPCs含量及其神经功能缺损程度的影响[J]. 中医药通报, 2017, 16(3): 39-42.
[24] 皮敏,陈鹏典,杨卓欣,等. 电针督脉联合人脐血间充质干细胞移植对脑缺血大鼠神经功能缺损及细胞凋亡的影响[J]. 时珍国医国药, 2014, 25(2): 492-495.
[25] 梁超,姜涛,王静芝,等. 不同变频组合电针预处理对急性脑缺血大鼠神经功能和脑皮质促红细胞生成素的影响[J]. 中国中医基础医学杂志, 2017, 23(5): 686-688.
[26] 蔡丽,刘毅,陆小青. 针刺对卒中后抑郁大鼠空间学习记忆功能及海马CA3区脑源性神经营养因子的影响[J]. 上海针灸杂志, 2016, 35(4): 462-465.
[27] 陈彬,张志涛,骆健明,等. 针刺治疗对缺血缺氧性脑病模型鼠学习与记忆能力的影响[J]. 中国医药, 2014, 9(8): 1159-1163.
[28] Burnstock G. Purinergic signaling in acupuncture [J]. Science, 2014, 346(6216): S23-S25.
[29] Weng ZJ, Wu LY, Zhou CL, et al. Effect of electro-acupuncture on P2X3 receptor regulation in the peripheral and central nervous systems of rats with visceral pain caused by irritable bowel syndrome [J]. Purinergic Signal, 2015, 11(3): 321-329.
[30] Guo X, Chen J, Yuan L, et al. Electro-acupuncture at He-Mu points reduces P2X4 receptor expression in visceral hypersensitivity [J]. Neural Regen Res, 2013, 8(22): 2069-2077.
[31] Liu W, Wang X, Zheng Y, et al. Electro-acupuncture inhibits inflammatory injury by targeting the miR-9-mediated NF-κB signaling pathway following ischemic stroke [J]. Mol Med Rep, 2016, 13(2): 1618-1626.
[32] Lan L, Tao J, Chen A, et al. Electro-acupuncture exerts anti-inflammatory effects in cerebral ischemia-reperfusion injured rats via suppression of the TLR4/NF-κB pathway [J]. Int J Mol Med, 2013, 31(1): 75-80.
[33] Chen SH, Sun H, Xu H, et al. [Effects of acupuncture of "Baihui"(GV 20) and "Zusanli"(ST 36) on expression of cerebral IL-1beta and TNF-alpha proteins in cerebral ischemia reperfusion injury rats] [J]. [in Chinese]. Zhen Ci Yan Jiu, 2012,37(6): 470-475.
[34] Cheng CY, Lin JG, Tang NY, et al. Electro-acupuncture-like stimulation at the Baihui (GV20) and Dazhui (GV14) acupoints protects rats against subacute-phase cerebral ischemia-reperfusion injuries by reducing S100B-mediated neurotoxicity [J]. PLoS One, 2014, 9(3): e91426.

电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响

Effects of Electroacupuncture at Baihui and Shenting on Learning and Memory and Expression of Purinoceptor P2X7 in Hippocampal CA1 after Cerebral Ischmeia-reperfusion in Rats

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