电针神庭、百会对脑缺血再灌注大鼠认知功能及Beclin-1表达的影响

doi:10.3969/j.issn.1006-9771.2018.01.009

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (1): 49-53.doi: 10.3969/j.issn.1006-9771.2018.01.009

电针神庭、百会对脑缺血再灌注大鼠认知功能及Beclin-1表达的影响

冯晓东, 史景, 湾明月, 刘承梅

河南中医药大学第一附属医院康复中心,河南郑州市 450000

收稿日期:2017-07-13 修回日期:2017-11-09 出版日期:2018-01-25 发布日期:2018-01-31
通讯作者: 刘承梅;E-mail:lcm0428@163.com。
作者简介:冯晓东(1968-),男,汉族,河南郑州市人,博士,教授,主任医师,主要研究方向：脑卒中中医康复基础与临床研究。
基金资助:
国家自然科学基金项目(No.14202214)

Effect of Electroacupuncture on Learning and Memory and Expression of Beclin-1 in Rats after Cerebral Ischemic-reperfusion

FENG Xiao-dong, SHI Jing, WAN Ming-yue, LIU Cheng-mei

Department of Rehabilitation, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450000, China

Received:2017-07-13 Revised:2017-11-09 Published:2018-01-25 Online:2018-01-31
Contact: LIU Cheng-mei.E-mail:lcm0428@163.com
Supported by:
National Natural Science Foundation of China (No. 14202214)

摘要/Abstract

摘要： 目的观察电针神庭、百会对脑缺血再灌注大鼠认知功能的影响,并探讨其可能机制。方法雄性Sprague-Dawley大鼠45只随机分为假手术组(n=15)、模型组(n=15)和电针组(n=15)。模型组和电针组采用线栓法制备脑缺血2 h再灌注模型。电针组术后2 h起电针百会、神庭共7 d。各组术后1 d、3 d、5 d、7 d行Longa评分;干预后4 d起,行Morris水迷宫测试,共4 d。干预后7 d,TTC染色检测脑梗死体积,Western blotting检测缺血侧Beclin-1蛋白表达。结果干预后3 d起,与模型组相比,电针组大鼠Longa评分降低(P<0.05)。各时间点,与模型组相比,电针组逃避潜伏期缩短(P<0.05),穿越平台次数提高(P<0.05)。与模型组相比,电针组脑梗死体积显著减小(F=7.651, P<0.001),Beclin-1表达增强(P<0.05)。结论电针百会、神庭穴可以改善脑缺血再灌注大鼠学习记忆能力,可能与调控自噬网络有关。

关键词: 脑缺血再灌注, 电针, 学习记忆, 自噬, Beclin-1, 大鼠

Abstract: Objective To observe the efficacy of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning and memory in rats after cerebral ischemia-reperfusion, and explore the possible mechanism.Methods A total of 45 male Sprague-Dawley rats were randomly divided into sham group (n=15), ischemia group (n=15) and electroacupuncture group (n=15). The left middle cerebral arteries of the latter two groups were occluded with the modified Longa's method for two hours and reperfused, and the electroacupuncture group received electroacupuncture at Baihui and Shenting once a day for seven days, then. They were assessed with Longa's score one, three, five and seven days after intervention. They were tested with Morris water maze since four days after intervention, once a day for four days. The cerebral infarction volume was measured with TTC staining seven days after intervention, while the protein expression of Beclin-1 was detected with Western blotting.Results Longa's score improved in the electroacupuncture group compared with that in the ischemia group since three days after intervention (P<0.05). The escape latency decreased and the times cross the area of platform increased in the electroacupuncture group compared with that in the ischemia group at every point (P<0.05). The cerebral infarction volume reduced (F=7.651, P<0.001) and the expression of Beclin-1 decreased (P<0.05) in the electroacupuncture group compared with that in the ischemia group.Conclusion Electroacupuncture can improve learning and memory in rats after cerebral ischemia-reperfusion, which may relate with regulation of autophagy network.

Key words: cerebral ischemia-reperfusion, electroacupuncture, learning and memory, autophagy, Beclin-1, rats

中图分类号:

R743.3

冯晓东, 史景, 湾明月, 刘承梅. 电针神庭、百会对脑缺血再灌注大鼠认知功能及Beclin-1表达的影响[J]. 《中国康复理论与实践》, 2018, 24(1): 49-53.

FENG Xiao-dong, SHI Jing, WAN Ming-yue, LIU Cheng-mei. Effect of Electroacupuncture on Learning and Memory and Expression of Beclin-1 in Rats after Cerebral Ischemic-reperfusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 49-53.

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电针神庭、百会对脑缺血再灌注大鼠认知功能及Beclin-1表达的影响

Effect of Electroacupuncture on Learning and Memory and Expression of Beclin-1 in Rats after Cerebral Ischemic-reperfusion

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