电针预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及凋亡相关蛋白表达的影响

doi:10.3969/j.issn.1006-9771.2018.01.010

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (1): 54-59.doi: 10.3969/j.issn.1006-9771.2018.01.010

电针预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及凋亡相关蛋白表达的影响

叶涛¹, 朱路文², 唐强², 李宏玉², 吴孝军¹, 陈晨², 姜云飞², 李佳帅¹

1.黑龙江中医药大学,黑龙江哈尔滨市 150040
2.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001

收稿日期:2017-11-12 修回日期:2017-12-04 出版日期:2018-01-25 发布日期:2018-01-31
通讯作者: 朱路文(1983-),男,副教授,主要研究方向：脑缺血预处理方案及机制研究;E-mail:zhuluwen1983@126.com。
作者简介:叶涛(1989-),男,汉族,福建漳平市人,博士研究生,主要研究方向：脑缺血电针、运动预处理方案及机制研究。
基金资助:
1.国家自然科学基金项目(No.81503666); 2.黑龙江省自然科学基金项目(No.QC2015103); 3.黑龙江省普通本科高等学校青年创新人才培养计划项目(No.201680)

Effects of Electroacupuncture Preconditioning on Apoptosis and Expression of Apoptosis-related Proteins in Rats after Cerebral Ischemia-reperfusion

YE Tao¹, ZHU Lu-wen², TANG Qiang², LI Hong-yu², WU Xiao-jun¹, CHEN Chen², JIANG Yun-fei², LI Jia-shuai¹

1. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China
2. The Second Hospital Affiliated Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China

Received:2017-11-12 Revised:2017-12-04 Published:2018-01-25 Online:2018-01-31
Contact: ZHU Lu-wen.E-mail:zhuluwen1983@126.com
Supported by:
National Natural Science Foundation of China (No. 81503666), Heilongjiang Natural Science Foundation (No. QC2015103) and Heilongjiang Program for Distinguished Young Talents in Higher Education (No. 201680)

摘要/Abstract

摘要： 目的探讨电针预处理对脑缺血再灌注后细胞凋亡的保护作用。方法雄性Sprague-Dawley大鼠72只随机分成假手术组(n=24)、模型组(n=24)和电针预处理组(n=24)。后两组采用改良Longa法制备大鼠缺血2 h再灌注模型,电针预处理组造模前连续电针百会2周。再灌注24 h后,采用改良神经损害严重程度评分(mNSS)进行评估,TTC染色观测脑梗死体积,TUNEL法观察缺血半暗区细胞凋亡情况,Western blotting检测缺血半暗区p53、Bcl-2、Bax蛋白表达。结果与模型组比较,电针预处理组mNSS评分降低(P<0.05),脑梗死体积缩小(P<0.05),TUNEL阳性细胞数减少(P<0.05),p53、Bax蛋白水平均降低(P<0.05),Bcl-2蛋白水平升高(P<0.05),Bax/Bcl-2比降低(P<0.05)。结论电针预处理对脑缺血再灌注大鼠有保护作用,可能与其抑制缺血半暗区p53蛋白表达,下调Bax/Bcl-2比值,从而减轻细胞凋亡有关。

关键词: 脑缺血再灌注, 电针, 预处理, 凋亡, p53, Bax, Bcl-2, 大鼠

Abstract: Objective To investigate the effect of electroacupuncture (EA) preconditioning on apoptosis after cerebral ischemia-reperfusion (I/R).Methods A total of 72 male Sprague-Dawley rats were randomly divided into sham group (n=24), model group (n=24) and EA group (n=24). The rats in latter two groups were occluded the right middle cerebral arteries for two hours and reperfused. EA group was treated with EA at Baihui (GV20) for two weeks before modeling. They were assessed with modified Neurological Severity Scores (mNSS) 24 hours after modeling. Then, the cerebral infarct volume was measured with TTC staining, the apoptosis was detected with TUENL assay, and the expression of p53, Bax and Bcl-2 proteins in ischemic penumbra was detected with Western blotting.Results Compared with the model group, the score of mNSS, cerebral infarct volume and the number of TUNEL-positive cells all significantly decreased (P<0.05) in EA group; while the expression of p53 and Bax proteins decreased (P<0.05), Bcl-2 increased (P<0.05), and Bax/Bcl-2 decreased (P<0.05).Conclusion EA preconditioning can induce tolerance to cerebral I/R injury, which might associate with the inhibition of p53 protein and down-regulation of the Bax/Bcl-2 ratio in ischemic penumbra, to inhibit cerebral cell apoptosis.

Key words: cerebral ischemia-reperfusion, electroacupuncture, preconditioning, apoptosis, p53, Bax, Bcl-2, rats

中图分类号:

R743.3

叶涛, 朱路文, 唐强, 李宏玉, 吴孝军, 陈晨, 姜云飞, 李佳帅. 电针预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及凋亡相关蛋白表达的影响[J]. 《中国康复理论与实践》, 2018, 24(1): 54-59.

YE Tao, ZHU Lu-wen, TANG Qiang, LI Hong-yu, WU Xiao-jun, CHEN Chen, JIANG Yun-fei, LI Jia-shuai. Effects of Electroacupuncture Preconditioning on Apoptosis and Expression of Apoptosis-related Proteins in Rats after Cerebral Ischemia-reperfusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 54-59.

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电针预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及凋亡相关蛋白表达的影响

Effects of Electroacupuncture Preconditioning on Apoptosis and Expression of Apoptosis-related Proteins in Rats after Cerebral Ischemia-reperfusion

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