《中国康复理论与实践》

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (2): 229-232.doi: 10.3969/j.issn.1006-9771.2018.02.021

• 康复工程与辅助技术 • 上一篇    下一篇

辅具中颈托的体外细胞毒性试验研究

栾会芹1, 2, 3, 4, 谷慧茹1, 2, 3, 4, 闫和平1, 2, 3, 张莹莹1, 2, 3, 樊瑜波1, 2, 3   

  1. 1.国家康复辅具研究中心,北京市 100176;
    2.北京市老年功能障碍康复辅助技术重点实验室,北京市 100176;
    3.民政部康复辅具分析评定重点实验室,北京市 100176;
    4.康复辅具评价重点实验室,北京市 100176
  • 收稿日期:2017-11-03 修回日期:2017-12-09 出版日期:2018-02-25 发布日期:2018-03-02
  • 通讯作者: 樊瑜波。E-mail: yubofan@buaa.edu.cn
  • 作者简介:栾会芹(1974-),女,汉族,山东莱芜市人,博士,主要研究方向：运动康复及康复辅具产品生物学评价。
  • 基金资助:
    1.国家重点研发计划项目(No. 2016YFB1101102); 2.中央级公益性科研院所基本科研业务费专项资金项目(No. 118009001000160001)

Cytotoxicity Test of Cervical Collar in Aids

LUAN Hui-qin1, 2, 3, 4, GU Hui-ru1, 2, 3, 4, YAN He-ping1, 2, 3, ZHANG Ying-ying1, 2, 3, FAN Yu-bo1, 2, 3   

  1. 1. National Research Center for Rehabilitation Technical Aids, Beijing 100176, China;
    2. Beijing Key Laboratory of Rehabilitation Technical Aids for Old-Age Disability, Beijing 100176, China;
    3. Key Laboratory of Rehabilitation Technical Aids Analysis and Identification of the Ministry of Civil Affairs, Beijing 100176, China;
    4. Key Laboratory of Rehabilitation Aids Evaluation, Beijing 100176, China
  • Received:2017-11-03 Revised:2017-12-09 Published:2018-02-25 Online:2018-03-02
  • Contact: FAN Yu-bo. E-mail:yubofan@buaa.edu.cn

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摘要： 目的 研究辅具中颈托的体外细胞毒性,调查中国市售颈托的细胞毒性现状。方法 参照GB/T 16886.5-2003和GB/T 16175-2008体外细胞毒性的实验原则,采用显微镜观察与噻唑蓝比色法,在小鼠成纤维细胞(L929株)上对4种不同品牌颈托进行细胞毒性检测。结果 颈托浸提浓度为0.1 g/ml时,颈托D的细胞毒性反应为3级,颈托A为2级,颈托B与颈托C的细胞毒性反应均为1级。颈托浸提液浓度为0.2 g/ml时,颈托A与颈托D的细胞毒性反应均为4级,而颈托B与颈托C的细胞毒性反应均为2级。结论 受试颈托均有不同程度的细胞毒性反应。颈托A与颈托D细胞毒性反应级别较高。

关键词: 辅具, 颈托, 细胞毒性, 噻唑蓝比色法

Abstract: Objective To study the cytotoxicity of cervical collar in rehabilitation technical aids and to investigate the cytotoxicity of domestic marketed products.Methods According to the experimental principle of GB/T 16886.5-2003 and GB/T 16175-2008, microscopic observation and Thiazole Blue Colorimetric methods were used to observe the toxicity of extract from four different cervical collars on the cells (L929).Results When the concentration of the extract was 0.1 g/ml, the cytotoxicity was grade three and grade two in the cervical collars D and A, respectively; and it was grade one both in cervical collars B and C. When the concentration of the extract was 0.2 g/ml, the cytotoxicity was grade four in cervical collars A and D, and was grade two in cervical collars B and C.Conclusion The cervical collars varied in the cytotoxicity, especially in cervical collars A and D.

Key words: aids, cervical collar, cytotoxicity, Thiazole Blue Colorimetry

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