维生素D对大鼠脑外伤海马组织自噬及细胞凋亡的影响

doi:10.3969/j.issn.1006-9771.2018.02.06

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (2): 148-152.doi: 10.3969/j.issn.1006-9771.2018.02.06

维生素D对大鼠脑外伤海马组织自噬及细胞凋亡的影响

姚佳¹, 朱莉¹, 郭鑫², 徐鹏飞¹, 江沛¹, 崔昌萌³

1.济宁市第一人民医院,山东济宁市272000;
2.华北理工大学,河北唐山市 063000;
3.济宁医学院附属医院,山东济宁市 272000

收稿日期:2017-08-03 修回日期:2017-09-11 出版日期:2018-02-25 发布日期:2018-03-02
通讯作者: 崔昌萌。E-mail: cmcuidr1989@163.com。
作者简介:姚佳(1983-),女,汉族,山东济宁市人,硕士,主管药师,主要研究方向：临床药学。通讯作者：崔昌萌,男,博士,主治医师,研究方向：脑创伤。
基金资助:
国家自然科学基金项目(No. 81602846)

Effects of Vitamin D on Autophagy and Apoptosis in Hippocampus of Rats with Traumatic Brain Injury

YAO Jia¹, ZHU Li¹, GUO Xin², XU Peng-fei¹, JIANG Pei¹, CUI Chang-meng³

1. First People's Hospital of Jining, Jining, Shandong 272000, China;
2. North China University of Science and Technology, Tangshan, Hebei 063000, China;
3. Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, China

Received:2017-08-03 Revised:2017-09-11 Published:2018-02-25 Online:2018-03-02
Contact: CUI Chang-meng. E-mail:cmcuidr1989@163.com
Supported by:
; Supported by National Natural Science Foundation of China (No. 81602846)

摘要/Abstract

摘要： 目的探讨低浓度维生素D₃(VDH)对脑外伤大鼠海马组织内自噬和凋亡的影响。方法 45只雄性Sprague-Dawley大鼠分为对照组(n=15)、模型组(n=15)和VDH组(n=15)。后两组制作脑外伤模型,VDH组于造模后30 min、24 h、48 h腹腔注射VDH 1 μg/kg。3 d后,Western blotting检测海马组织微管相关蛋白轻链3(LC3)和p62的表达,TUNEL检测凋亡细胞数。三组均于造模后第5、6、7天进行Morris水迷宫测试,记录逃避潜伏期及游泳速度。结果模型组TUNEL阳性细胞数多于对照组(P<0.05),VDH组TUNEL阳性细胞数少于模型组(P<0.05)。模型组LC3Ⅱ/LC3Ⅰ和p62表达均明显高于对照组(P<0.01),VDH组明显低于模型组(P<0.01)。模型组逃避潜伏期明显长于对照组(P<0.01),VDH组逃避潜伏期短于模型组(P<0.05)。三组大鼠第5、6、7天的平均游泳速度无显著性差异(F=0.530, P>0.05)。结论 VDH对脑外伤大鼠有神经保护作用,可能与脑外伤后影响海马组织LC3、p62蛋白的表达,发挥抗凋亡作用有关。

关键词: 脑外伤, 活性维生素D, 自噬, 凋亡, 大鼠

Abstract: Objective To explore the effect of vitamin D hormone (VDH) on autophagy and apoptosis in hippocampus of rats with traumatic brain injury (TBI).Methods A total of 45 male Sprague-Dawley rats were divided into control group (n=15), model group (n=15) and VDH group (n=15). The TBI model was established in the model group and VDH group. VDH group was injected with VDH 1 μg /kg 30 minutes, 24 hours and 48 hours after modeling, respectively. The expression of microtubule associated protein 1 light chain 3 (LC3) and p62 was detected with Western blotting, and the number of apoptotic cells were detected with TUNEL three days after modeling. All groups were tested with Morris water maze on the fifth, sixth and seventh days.Results The number of TUNEL positive cells in hippocampus was more in the model group than in the control group (P<0.05), and was lower in VDH group than in the model group (P<0.05), as well as the expression of LC3II/LC3I and p62 (P<0.05). The escape latency was longer in the model group than in the control group (P<0.05), and was shorter in VDH group than in the model group (P<0.05). There was no significant difference in the swimming velocity on the fifth, sixth, and seventh days among three groups (F=0.530, P>0.05).Conclusion VDH has potential neuroprotective effects on TBI, which might be associated with its anti-apoptosis effect on the expression of LC3 and p62 proteins in hippocampus after TBI.

Key words: traumatic brain injury, active form of vitamin D hormone, autophagy, apoptosis, rats

中图分类号:

R651.1

姚佳, 朱莉, 郭鑫, 徐鹏飞, 江沛, 崔昌萌. 维生素D对大鼠脑外伤海马组织自噬及细胞凋亡的影响[J]. 《中国康复理论与实践》, 2018, 24(2): 148-152.

YAO Jia, ZHU Li, GUO Xin, XU Peng-fei, JIANG Pei, CUI Chang-meng. Effects of Vitamin D on Autophagy and Apoptosis in Hippocampus of Rats with Traumatic Brain Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(2): 148-152.

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维生素D对大鼠脑外伤海马组织自噬及细胞凋亡的影响

Effects of Vitamin D on Autophagy and Apoptosis in Hippocampus of Rats with Traumatic Brain Injury

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