运动预处理对脑缺血再灌注大鼠线粒体ATP敏感性钾通道蛋白及细胞凋亡的影响

doi:10.3969/j.issn.1006-9771.2018.05.001

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (5): 497-501.doi: 10.3969/j.issn.1006-9771.2018.05.001

• 专题缺血性脑损伤的评定与康复 • 下一篇

运动预处理对脑缺血再灌注大鼠线粒体ATP敏感性钾通道蛋白及细胞凋亡的影响

李宏玉^1,2, 唐强², 朱路文², 王雪¹, 郑婷婷¹, 陈玉红¹, 赵一点¹, 尹侠¹

1.黑龙江中医药大学,黑龙江哈尔滨市 150040;
2.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001

收稿日期:2018-01-14 修回日期:2018-03-27 出版日期:2018-05-25 发布日期:2018-05-24
通讯作者: 唐强。E-mail: tangqiang1963@163.com。
作者简介:李宏玉(1991-),女,汉族,黑龙江鸡西市人,博士研究生,医师,主要研究方向：运动预处理的脑保护机制。通讯作者：唐强(1963-),男,教授,博士生导师,主要研究方向：脑卒中康复基础与临床研究。
基金资助:
1.中国博士后科学基金项目(No. 2017M611411); 2.黑龙江省博士后基金项目(No. LBH-Z16195); 3.哈尔滨市科技创新人才研究专项资金项目(No. RC2017QN003087)

Effect of Exercise Preconditioning on Cell Apoptosis and Expression of Mitochondrial ATP-sensitive Potassium Channels Proteins in Rats after Cerebral Ischemia-reperfusion

LI Hong-yu^1,2, TANG Qiang², ZHU Lu-wen², WANG Xue¹, ZHENG Ting-ting¹, CHEN Yu-hong¹, ZHAO Yi-dian¹, YIN Xia¹

1. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China;
2. Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China

Received:2018-01-14 Revised:2018-03-27 Published:2018-05-25 Online:2018-05-24
Contact: TANG Qiang. E-mail: tangqiang1963@163.com
Supported by:
Supported by　China Postdoctoral Science Fund (No. 2017M611411), Heilongjiang Postdoctoral Fund (No. LBH-Z 16195), and Harbin Science and Technology Innovation Talent Research Fund (No. RC2017QN003087)

摘要/Abstract

摘要： 目的探讨运动预处理对脑缺血再灌注大鼠神经功能缺损的影响及可能机制。方法健康Sprague-Dawley大鼠36只随机分为假手术组、模型组和运动预处理组,每组12只。后两组采用改良线栓法制备脑缺血120 min再灌注模型。再灌注2 h、12 h、24 h后,采用Longa评分法评定;再灌注24 h后,采用Western blotting检测线粒体ATP敏感性钾(mitoK_ATP)通道蛋白内向整流性钾离子通道6.2 (Kir6.2)和磺脲类受体1 (SUR1)的表达,TUNEL染色检测神经细胞凋亡。结果脑缺血再灌注24 h后,与模型组比较,运动预处理组Longa评分降低(P<0.05), Kir6.2、SUR1蛋白水平下降(P<0.05),细胞凋亡减少(P<0.05)。结论运动预处理可能通过调节mitoK_ATP通道蛋白表达,降低细胞凋亡,从而改善脑缺血再灌注后神经功能。

关键词: 脑缺血再灌注, 运动, 预处理, 线粒体ATP敏感性钾通道, 细胞凋亡, 大鼠

Abstract: Objective To explore the effect and mechanism of exercise preconditioning on neurological deficits in rats after cerebral ischemia-reperfusion. Methods Thirty-six healthy Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and exercise preconditioning group (n=12). The latter two groups were occluded middle cerebral artery for 120 minutes and reperfused with modified suture method. The rats were evaluated with Longa's score two, twelve and 24 hours after reperfusion. The expression of mitochondrial ATP-sensitive potassium (mitoK_ATP) channel proteins inwardly rectifying potassium channel (Kir6.2) and sulphonylurea receptor 1 (SUR1) were detected with Western blotting and the cerebral cell apoptosis was detected with TUENL assay 24 hours after reperfusion. Results Compared with the model group, the Longa's score decreased in the exercise preconditioning group 24 hours after reperfusion (P<0.05), while the expression of Kir6.2 and SUR1 decreased (P<0.05), and TUNEL-positive cells decreased (P<0.05).Conclusion Exercise preconditioning may improve neurological function after cerebral ischemia-reperfusion, which may associate with inhibiting the expression of mitoK_ATP channel proteins and cell apoptosis.

Key words: cerebral ischemia-reperfusion, exercise, preconditioning, mitochondrial ATP-sensitive potassium channels, cell apoptosis, rats

中图分类号:

R743.3

李宏玉, 唐强, 朱路文, 王雪, 郑婷婷, 陈玉红, 赵一点, 尹侠. 运动预处理对脑缺血再灌注大鼠线粒体ATP敏感性钾通道蛋白及细胞凋亡的影响[J]. 《中国康复理论与实践》, 2018, 24(5): 497-501.

LI Hong-yu, TANG Qiang, ZHU Lu-wen, WANG Xue, ZHENG Ting-ting, CHEN Yu-hong, ZHAO Yi-dian, YIN Xia. Effect of Exercise Preconditioning on Cell Apoptosis and Expression of Mitochondrial ATP-sensitive Potassium Channels Proteins in Rats after Cerebral Ischemia-reperfusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(5): 497-501.

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运动预处理对脑缺血再灌注大鼠线粒体ATP敏感性钾通道蛋白及细胞凋亡的影响

Effect of Exercise Preconditioning on Cell Apoptosis and Expression of Mitochondrial ATP-sensitive Potassium Channels Proteins in Rats after Cerebral Ischemia-reperfusion

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