不同剂量人参皂甙Rb1对大鼠脊髓缺血再灌注损伤及survivin的作用

doi:10.3969/j.issn.1006-9771.2018.05.006

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (5): 520-525.doi: 10.3969/j.issn.1006-9771.2018.05.006

不同剂量人参皂甙Rb1对大鼠脊髓缺血再灌注损伤及survivin的作用

程斌¹, 宋焕瑾¹, 李锋涛¹, 林磊², 薛建利¹, 吴昊¹, 叶劲涛¹

1.西安交通大学医学院第二附属医院骨科,陕西西安市 710004;
2.汉中市中心医院骨科,陕西汉中市 723000

收稿日期:2017-12-27 修回日期:2018-04-04 出版日期:2018-05-25 发布日期:2018-05-24
通讯作者: 叶劲涛。E-mail: yejintao@163.com
作者简介:程斌(1961-),男,汉族,陕西兴平市人,博士,教授,主要研究方向：脊柱外科。通讯作者：叶劲涛(1993-),男,汉族,陕西西安市人,硕士研究生,主要研究方向：脊柱外科。
基金资助:
陕西省自然科学基金面上项目(No. 2014JM2_8157; No. 2016JM8129)

Effects of Ginsenoside Rb1 in Different Dosages on Spinal Cord Ischemia-reperfusion Injury and Expression of Survivin in Rats

CHENG Bin¹, SONG Huan-jin¹, LI Feng-tao¹, LIN Lei², XUE Jian-li¹, WU Hao¹, YE Jin-tao¹

1. Department of Orthopedics, the 2nd Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China;
2. Department of Orthopedics, Hanzhong Central Hospital, Hanzhong, Shaanxi 723000, China

Received:2017-12-27 Revised:2018-04-04 Published:2018-05-25 Online:2018-05-24
Contact: YE Jin-tao. E-mail: yejintao@163.com
Supported by:
Supported by Shaanxi Natural Science Foundation (General) (No. 2014JM2_8157 and No. 2016JM8129)

摘要/Abstract

摘要： 目的观察不同剂量人参皂甙Rb1预处理对于大鼠脊髓缺血再灌注后的干预效果,并探讨其可能机制。方法 Sprague-Dawley大鼠随机分为假手术组,模型组以及药物10 mg/kg (D10)、20 mg/kg (D20)、40 mg/kg (D40)、80 mg/kg (D80)组,每组12只。药物组于造模前30 min腹腔注射相应剂量人参皂甙Rb1。构建大鼠脊髓缺血10 min再灌注模型。造模后48 h行BBB评分,HE染色及TUNEL检测观察细胞凋亡,免疫组化法观察survivin蛋白表达。结果各药物组BBB评分均高于模型组(P<0.05),以D40和D80组最高;survivin蛋白表达均高于模型组(P<0.05),以D40和D80组最高;神经细胞凋亡少于模型组(P<0.05),以D40和D80组最少。结论人参皂甙Rb1可以通过促进survivin的表达,抑制大鼠脊髓缺血再灌注造成的细胞凋亡,保护神经功能;在一定范围内,人参皂甙Rb1对脊髓缺血再灌注损伤的保护作用具有剂量依赖性。

关键词: 脊髓损伤, 缺血再灌注, 人参皂甙Rb1, survivin, 大鼠

Abstract: Objective To observe the effects of different dosages of ginsenoside Rb1 preconditioning on spinal cord ischemia-reperfusion injury in rats, and the possible mechanism. Methods Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12), and 10 mg/kg (D10, n=12), 20 mg/kg (D20, n=12), 40 mg/kg (D40, n=12) and 80 mg/kg (D80, n=12) drug groups. Spinal cord ischemia for ten minutes and reperfusion model was established, and the drug groups were injected ginsenoside Rb1 intraperitoneally in their dosages 30 minutes before modeling. They were assessed with BBB score 48 hours after reperfusion, and then were sacrificed for HE staining, TUNEL staining and immunohistochemistry staining of survivin.Results The BBB score was more in the drug groups than in the model group (P<0.05), and was the most in D40 and D80 groups. The expression of survivin was more in the drug groups than in the model group (P<0.05), and was the most in D40 and D80 groups. The apoptosis of neurons was less in the drug groups than in the model group (P<0.05), and was the least in D40 and D80 groups.Conclusion The ginsenoside Rb1 could promote the expression of survivin, inhibit apoptosis of neurons, to protect the neural function, in dose-dependent manner somehow.

Key words: spinal cord injury, ischemia-reperfusion, ginsenoside Rg1, survivin, rats

中图分类号:

R651.2

程斌, 宋焕瑾, 李锋涛, 林磊, 薛建利, 吴昊, 叶劲涛. 不同剂量人参皂甙Rb1对大鼠脊髓缺血再灌注损伤及survivin的作用[J]. 《中国康复理论与实践》, 2018, 24(5): 520-525.

CHENG Bin, SONG Huan-jin, LI Feng-tao, LIN Lei, XUE Jian-li, WU Hao, YE Jin-tao. Effects of Ginsenoside Rb1 in Different Dosages on Spinal Cord Ischemia-reperfusion Injury and Expression of Survivin in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(5): 520-525.

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不同剂量人参皂甙Rb1对大鼠脊髓缺血再灌注损伤及survivin的作用

Effects of Ginsenoside Rb1 in Different Dosages on Spinal Cord Ischemia-reperfusion Injury and Expression of Survivin in Rats

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