脉冲射频对坐骨神经慢性压迫大鼠降钙素基因相关肽表达的远期影响

doi:10.3969/j.issn.1006-9771.2018.05.007

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (5): 526-529.doi: 10.3969/j.issn.1006-9771.2018.05.007

脉冲射频对坐骨神经慢性压迫大鼠降钙素基因相关肽表达的远期影响

付淼¹, 任浩², 罗芳²

1.北京大学肿瘤医院暨北京市肿瘤防治研究所,恶性肿瘤发病机制及转化研究教育部重点实验室麻醉科,北京市 100142;
2.首都医科大学附属北京天坛医院麻醉科,北京市 100050

收稿日期:2018-02-07 修回日期:2018-03-14 出版日期:2018-05-25 发布日期:2018-05-24
通讯作者: 罗芳。E-mail: luofangwt@yahoo.com
作者简介:付淼(1988-),女,汉族,北京市人,医师,主要研究方向：疼痛治疗与麻醉。通讯作者：罗芳(1971-),女,四川人,主任医师、教授,主要研究方向：急慢性疼痛的治疗。
基金资助:
北京市卫生系统高层次人才培养计划(学科骨干)项目(No. 2014-3-035)

Long-term Effect of Pulsed Radiofrequency on Expression of Calcitonin Gene-related Peptide in Chronic Constriction Injured Rats

FU Miao¹, REN Hao², LUO Fang²

1. Department of Anesthesiology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing 100142, China;
2. Department of Anesthesiology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;

Received:2018-02-07 Revised:2018-03-14 Published:2018-05-25 Online:2018-05-24
Contact: LUO Fang. E-mail: luofangwt@yahoo.com
Supported by:
Supported by　Beijing Health System Excellent Professional Program (Core) (No. 2014-3-035)

摘要/Abstract

摘要： 目的探讨脉冲射频对坐骨神经慢性压迫模型大鼠背根神经节中降钙素基因相关肽(CGRP)表达的长期影响。方法 Sprague-Dawley大鼠36只随机分为假手术组(n=12)、模型组(n=12)和治疗组(n=12)。模型组和治疗组结扎坐骨神经,治疗组予脉冲射频治疗坐骨神经结扎部位120 s。治疗前及治疗后7 d、14 d、21 d、28 d测量缩足反射阈值(HWT),治疗后28 d取大鼠右L_4-6背根神经节,采用RT-qPCR和ELISA方法检测CGRP表达。结果治疗后治疗组大鼠HWT逐渐升高,7 d起即高于模型组(P<0.05),21 d、28 d与假手术组无显著性差异(P>0.05)。治疗组CGRP转录及翻译水平均明显低于模型组(P<0.01)。结论脉冲射频治疗可长时间缓解神经病理性疼痛,可能与下调背根神经节中CGRP表达有关。

关键词: 神经病理性疼痛, 坐骨神经, 脉冲射频, 降钙素基因相关肽, 大鼠

Abstract: Objective To investigate the long-term effect of pulsed radiofrequency on the expression of calcitonin gene-related peptide (CGRP) in dorsal root ganglion of rats with chronic constriction injury. Methods A total of 36 Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and treatment group (n=12). The sciatic nerve was ligated in the model group and the treatment group, and the treatment group was treated with pulsed radiofrequency on the injured nerve for 120 seconds. Hindpaw withdrawl threshold (HWT) was measured before treatment, and seven days, 14 days, 21 days and 28 days after treatment. After HWT, the right L_4-6 dorsal root ganglions were tested the CGRP expression using RT-qPCR and ELISA. Results HWT gradually increased in the treatment group after treatment, and was higher than that in the model group since seven days after treatment (P<0.05), similar to that of the sham group 21 days and 28 days after treatment (P>0.05). The transcription and translation levels of CGRP were both significantly lower in the treatment group than in the model group (P<0.01).Conclusion Pulsed radiofrequency might relieve the neuropathic pain through reducing CGRP expression in dorsal root ganglion in a long term.

Key words: neuropathic pain, sciatic nerve, pulsed radiofrequency, calcitonin gene-related peptide, rats

中图分类号:

R745.4

付淼, 任浩, 罗芳. 脉冲射频对坐骨神经慢性压迫大鼠降钙素基因相关肽表达的远期影响[J]. 《中国康复理论与实践》, 2018, 24(5): 526-529.

FU Miao, REN Hao, LUO Fang. Long-term Effect of Pulsed Radiofrequency on Expression of Calcitonin Gene-related Peptide in Chronic Constriction Injured Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(5): 526-529.

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脉冲射频对坐骨神经慢性压迫大鼠降钙素基因相关肽表达的远期影响

Long-term Effect of Pulsed Radiofrequency on Expression of Calcitonin Gene-related Peptide in Chronic Constriction Injured Rats

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