大鼠脊髓损伤后胃肠动力的X线研究

doi:10.3969/j.issn.1006-9771.2018.06.005

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (6): 645-649.doi: 10.3969/j.issn.1006-9771.2018.06.005

• 专题脊髓损伤的机制及干预 • 上一篇下一篇

大鼠脊髓损伤后胃肠动力的X线研究

蔡畅,李建军,高峰,杨明亮,杜良杰,杨德刚,刘长彬,李大鹏,张文豪,徐珮珮

1. 首都医科大学康复医学院,北京市 100068;
2. 中国康复研究中心北京博爱医院,脊柱脊髓神经功能重建科,北京市 100068;
3. 北京脑重大疾病研究院神经损伤与修复研究所,北京市 100068;
4. 北京市神经损伤与康复重点实验室,北京市 100068

收稿日期:2018-05-09 修回日期:2018-05-18 出版日期:2018-06-25 发布日期:2018-06-28
通讯作者: 李建军。E-mail: crrc100@163.com
作者简介:蔡畅(1990-),女,汉族,河北保定市人,硕士研究生,主要研究方向：脊柱脊髓损伤康复。
基金资助:
1.国家自然科学基金面上项目(No. 81271366); 2.北京市科技计划重大项目(No. D161100002816004); 3.首都卫生发展科研专项重点攻关项目(No.首发2018-1-6011); Supported by National Natural Science Foundation of China (General) (No. 81271366), Beijing Science and Technology Program (Major) (No. D161100002816004) and Capital Health Development Research Project (No.首发2018-1-6011)

Gastrointestinal Motility after Spinal Cord Injury in Rats: An X-ray Study

CAI Chang, LI Jian-jun, GAO Feng, YANG Ming-liang, DU Liang-jie, YANG De-gang, LIU Chang-bin, LI Da-peng, ZHANG Wen-hao, XU Pei-pei

1. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China;
2. Department of Spinal and Neural Function Reconstruction, Beijing 100068, China;
3. Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100068, China;
4. Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing 100068, China

Received:2018-05-09 Revised:2018-05-18 Published:2018-06-25 Online:2018-06-28
Contact: LI Jian-jun. E-mail: crrc100@163.com

摘要/Abstract

摘要： 目的探索大鼠脊髓损伤所致神经源性肠功能障碍的肠道功能动态改变。方法 24只雌性Sprague-Dawley大鼠分为对照组(n=12)和脊髓损伤组(n=12)。采用动脉瘤夹(标定力为70 g)钳夹60 s制作T₁₀脊髓损伤模型;对照组只暴露硬脊膜。分别于造模前和造模后4周,X线造影观察肠道功能的动态改变,HE染色观察结肠病理改变。结果与对照组相比,脊髓损伤组胃排空及肠道传输功能明显降低(P<0.05)。结论采用动脉瘤夹能成功复制大鼠脊髓损伤模型。大鼠脊髓损伤后出现神经源性肠功能障碍,胃排空及肠道传输功能明显减弱。

关键词: 脊髓损伤, 神经源性肠功能障碍, X线, 大鼠

Abstract: Objective To study the dynamic changes of the intestinal function of neurogenic bowel dysfunction rats caused by spinal cord injury using X-ray radiography.Methods Twenty-four female Sprague-Dawley rats were divided into control group (n=12) and spinal cord injury group (n=12). The T₁₀ spinal cord injury model was established using aneurysm clip (70 grams calibration force) for 60 seconds. The control group exposed the dura only. X-ray Barium was used to observe the dynamic changes of intestinal function, and HE staining was used to observe the pathology of the colon before and four weeks after modeling.Results Compared with the control group, gastric emptying and intestinal transit function significantly reduced in the spinal cord injury group (P<0.05).Conclusion The spinal cord injury model can be successfully duplicated with aneurysm clip in rats; neurogenic bowel dysfunction occurs after spinal cord injury, gastric emptying and intestinal transit function are weakened.

Key words: spinal cord injury, neurogenic bowel dysfunction, X-ray, rats

中图分类号:

R651.2

蔡畅,李建军,高峰,杨明亮,杜良杰,杨德刚,刘长彬,李大鹏,张文豪,徐珮珮. 大鼠脊髓损伤后胃肠动力的X线研究[J]. 《中国康复理论与实践》, 2018, 24(6): 645-649.

CAI Chang, LI Jian-jun, GAO Feng, YANG Ming-liang, DU Liang-jie, YANG De-gang, LIU Chang-bin, LI Da-peng, ZHANG Wen-hao, XU Pei-pei. Gastrointestinal Motility after Spinal Cord Injury in Rats: An X-ray Study[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(6): 645-649.

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大鼠脊髓损伤后胃肠动力的X线研究

Gastrointestinal Motility after Spinal Cord Injury in Rats: An X-ray Study

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