督脉灸对衰老模型大鼠骨骼肌泛素蛋白酶体途径的影响

doi:10.3969/j.issn.1006-9771.2018.09.005

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (9): 1018-1021.doi: 10.3969/j.issn.1006-9771.2018.09.005

督脉灸对衰老模型大鼠骨骼肌泛素蛋白酶体途径的影响

林建平^1,2, 王喜¹, 刘午龙^2,3, 陈艺敏¹, 陈少清^2,3, 王诗忠⁴

1.福建中医药大学附属康复医院,福建福州市 350003;
2.福建省康复技术重点实验室,福建福州市 350003;
3.福建中医药大学,福建福州市 350122;
4.福建医科大学,福建福州市 350122

收稿日期:2018-04-05 修回日期:2018-06-28 出版日期:2018-09-20 发布日期:2018-09-27
通讯作者: 陈少清。E-mail: chenshaoqd@163.com
作者简介:林建平(1985-),女,汉族,福建长乐市人,博士,主治医师,主要研究方向：脊柱与骨关节基础与临床研究。通讯作者：陈少清(1983-),男,博士,讲师。
基金资助:
1.福建省科技厅科技平台建设项目(No. 2015Y2001); 2.国家自然科学基金项目(No. 81250031)

Effects of Du Meridian Moxibustion on Ubiquitin Proteasome Pathway in Skeletal Muscle of Aging Rats

LIN Jian-ping^1,2, WANG Xi¹, LIU Wu-long^2,3, CHEN Yi-min¹, CHEN Shao-qing^2,3, WANG Shi-zhong⁴

1. Affiliated Rehabilitation Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350003, China;
2. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350003, China;
3. Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China;
4. Fujian Medical University, Fuzhou, Fujian 350122, China

Received:2018-04-05 Revised:2018-06-28 Published:2018-09-20 Online:2018-09-27
Contact: CHEN Shao-qing. E-mail: chenshaoqd@163.com
Supported by:
Supported by Fujian Science and Technology Department Science and Technology Platform Construction Project (No. 2015Y2001) and National Natural Science Foundation of China (No. 81250031)

摘要/Abstract

摘要： 目的探讨督脉灸改善骨骼肌衰老的泛素蛋白酶体途径机制。方法 Sprague-Dawley大鼠30只随机分为空白组、模型组和督脉灸组各10只。模型组和督脉灸组每天颈背部皮下注射D-半乳糖125 mg/kg,共6周,空白组皮下注射等量生理盐水。随后督脉灸组艾灸大椎穴至腰俞穴之间督脉,每天20 min,共20 d。剥离双侧竖脊肌,Western blotting检测泛素、肌环指蛋白1 (MuRF1)、肌萎缩F-box蛋白(MAFbx)蛋白水平。结果与空白组相比,模型组泛素、MuRF1、MAFbxD-半乳糖蛋白表达升高(P<0.05);与模型组相比,督脉灸组泛素、MuRF1和MAFbx蛋白表达下降(P<0.05)。结论督脉灸可通过对泛素蛋白酶体途径的调控,减少蛋白降解,延缓骨骼肌萎缩。

关键词: 督脉灸, 骨骼肌, 衰老, 泛素, 大鼠

Abstract: Objective To study the role of ubiquitin proteasome pathway in Du Meridian moxibustion for skeletal muscle aging. Methods A total of 30 Sprague-Dawley rats were randomly separated into control group (n=10), model group (n=10) and moxibustion group (n=10). The model group and the moxibustion group were subcutaneously injected D-galactose 125 mg/kg daily for six weeks, while the control group was injected the same volume of saline. Then the moxibustion group was cauterized on Du Meridian 20 minutes a day for 20 days. The expression of ubiquitin, muscle RING finger 1 (MuRF1) and muscle atrophy F-box (MAFbx) in erector spinae were tested with Western blotting. Results The expression of ubiquitin, MuRF1 and MAFbx increased in the model group compared with that of the control group (P<0.05), decreased in the moxibustion group compared with that of the model group (P<0.05).Conclusion The Du Meridian moxibustion may regulate ubiquitin proteasome pathway to inhibit the degradation of protein, and delay muscular atrophy.

Key words: Du Meridian moxibustion, skeletal muscle, aging, ubiquitin, rats

中图分类号:

R746.4

林建平, 王喜, 刘午龙, 陈艺敏, 陈少清, 王诗忠. 督脉灸对衰老模型大鼠骨骼肌泛素蛋白酶体途径的影响[J]. 《中国康复理论与实践》, 2018, 24(9): 1018-1021.

LIN Jian-ping, WANG Xi, LIU Wu-long, CHEN Yi-min, CHEN Shao-qing, WANG Shi-zhong. Effects of Du Meridian Moxibustion on Ubiquitin Proteasome Pathway in Skeletal Muscle of Aging Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(9): 1018-1021.

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督脉灸对衰老模型大鼠骨骼肌泛素蛋白酶体途径的影响

Effects of Du Meridian Moxibustion on Ubiquitin Proteasome Pathway in Skeletal Muscle of Aging Rats

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