嗅黏膜神经干细胞对大鼠神经元凋亡的影响

doi:10.3969/j.issn.1006-9771.2018.09.007

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (9): 1027-1033.doi: 10.3969/j.issn.1006-9771.2018.09.007

嗅黏膜神经干细胞对大鼠神经元凋亡的影响

杨新乐¹, 王明¹, 杨成伟², 张亚强¹, 徐雅洁³, 巩栋², 甄平²

1.兰州大学第二医院,甘肃兰州市 730000;
2.兰州总医院全军骨科中心,甘肃兰州市 730050;
3.河南省荣军医院,河南新乡市 453000

收稿日期:2018-06-02 修回日期:2018-06-22 出版日期:2018-09-20 发布日期:2018-09-27
通讯作者: 杨成伟。E-mail: chengwyang@aliyun.com
作者简介:杨新乐(1992-),男,满族,河南洛阳市人,硕士研究生,医师,主要研究方向：脊柱脊髓损伤。通讯作者：杨成伟,主治医师,博士,博士后,主要研究方向：脊柱脊髓损伤。
基金资助:
1.国家自然科学基金项目(No. 81601905); 2.中国博士后科学基金项目(No. 2017M623433)

Effect of Olfactory Mucosal Neural Stem Cells on Neuronal Apoptosis in Rats

YANG Xin-le¹, WANG Ming¹, YANG Cheng-wei², ZHANG Ya-qiang¹, XU Ya-jie³, GONG Dong², ZHEN Ping²

1. The Second Hospital of Lanzhou University, Lanzhou, Gansu 730000, China;
2. Department of Orthopaedic, the Lanzhou General Hospital of PLA, Lanzhou, Gansu 730050, China;
3. Henan Rongjun Hospital, Xinxiang, Henan 453000, China

Received:2018-06-02 Revised:2018-06-22 Published:2018-09-20 Online:2018-09-27
Contact: YANG Cheng-wei. E-mail: chengwyang@aliyun.com
Supported by:
Supported by National Natural Science Foundation of China (No. 81601905) and China Postdoctoral Science Foundation (No. 2017M623433)

摘要/Abstract

摘要： 目的探讨嗅黏膜神经干细胞(OM-NSCs)对大鼠神经元凋亡的影响。方法从雄性Sprague-Dawley大鼠分离提取OM-NSCs,从Sprague-Dawley胎鼠分离神经元。体外培养后,神经元分为空白组、对照组和实验组。空白组正常培养,对照组加白细胞介素-1β (IL-1β)刺激24 h后正常培养,实验组于IL-1β刺激后加OM-NSCs共培养。24 h后使用TUNEL和微管结合蛋白2 (MAP2)抗体双标染色观察神经元凋亡。结果空白组仅有少量细胞凋亡,对照组出现大量凋亡细胞,实验组凋亡细胞数较对照组明显减少(F=39.764, P<0.01)。结论 OM-NSCs能抑制大鼠神经元凋亡,具有神经保护作用。

关键词: 嗅黏膜神经干细胞, 神经元, 凋亡, 大鼠

Abstract: Objective To investigate the effect of olfactory mucosal neural stem cells (OM-NSCs) on neuronal apoptosis in rats. Methods OM-NSCs were isolated from adult male Sprague-Dawley rats, and neurons were isolated from fetal Sprague-Dawley rats. The neurons were divided into blank group, control group and experiment group after culture in vitro, which were further cultured in nomal, cultured in nomal 24 hours after stimulation of interleukin-1β (IL-1β), and cultured with OM-NSCs 24 hours after stimulation of IL-1β, respectively, for 24 hours. The apoptosis of neurons was observed with double staining of TUNEL and microtubule-associated protein 2 antibodies. Results There were few apoptotic cells in the blank group, and very many in the control group; the apoptotic cells were fewer in the experiment group than in the control group (F=39.764, P<0.01).Conclusion OM-NSCs can significantly inhibit neuronal apoptosis, which may play a role in neuroprotection.

Key words: olfactory mucous neural stem cells, neurons, apoptosis, rats

中图分类号:

R651.2

杨新乐, 王明, 杨成伟, 张亚强, 徐雅洁, 巩栋, 甄平. 嗅黏膜神经干细胞对大鼠神经元凋亡的影响[J]. 《中国康复理论与实践》, 2018, 24(9): 1027-1033.

YANG Xin-le, WANG Ming, YANG Cheng-wei, ZHANG Ya-qiang, XU Ya-jie, GONG Dong, ZHEN Ping. Effect of Olfactory Mucosal Neural Stem Cells on Neuronal Apoptosis in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(9): 1027-1033.

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嗅黏膜神经干细胞对大鼠神经元凋亡的影响

Effect of Olfactory Mucosal Neural Stem Cells on Neuronal Apoptosis in Rats

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