高脂饮食诱导肥胖对小鼠缺血性脑损伤的影响

doi:10.3969/j.issn.1006-9771.2018.10.006

摘要/Abstract

摘要： 目的探讨饮食诱导的肥胖对小鼠缺血性脑损伤的影响。方法 60只雄性C57/BL6小鼠分别经高脂饮食(高脂饮食组,n=30)或正常饮食(正常饮食组,n=30) 3个月,每组再分别分为假手术组和模型组两个亚组,每个亚组15只。模型组采用大脑中动脉阻塞(MCAO)进行永久性脑缺血。采用神经行为学评分法评估各组神经损伤情况;采用2,3,5-氯化三苯基四氮唑(TTC)染色法检测各组小鼠的脑梗死体积;采用Western blotting检测各组缺血大脑皮层去乙酰化酶(Sirt1)、Wnt和凋亡诱导因子(AIF)蛋白表达。结果造模后7 d,高脂饮食组神经行为学评分高于正常饮食组(t=10.053, P<0.05),两组脑梗死体积无显著性差异(t=6.872, P>0.05);高脂饮食小鼠缺血大脑皮层Sirt1表达高于正常饮食小鼠(t=8.462, P<0.05),Wnt表达明显低于正常饮食小鼠(t=17.752, P<0.01),细胞核中AIF表达高于正常饮食小鼠(t=8.471, P<0.05)。结论肥胖可影响小鼠缺血性脑损伤后恢复,可能与Wnt信号通路减弱有关。

关键词: 缺血性脑损伤, 肥胖, 小鼠

Abstract: Objective To investigate the effect of diet-induced obesity on ischemia brain injury in mice.Methods A total of 60 male C57/Bl6 mice were treated with high-fat (high-fat group, n=30) or normal diet (control group, n=30) for three months, and each group was further divided into sham group and model group, with 15 mice in each subgroup. The model group was established the middle cerebral artery occlusion model. The nerve injury was evaluated with Neurologic Severity Score (NSS), the infarct volume was evaluated by 2,3,5- chlorinated three phenyl tetrazolium chloride (TTC) staining, the expression of sirtuin (Sirt1), Wnt and apoptosis inducing factor (AIF) were detected with Western blotting seven days after modeling.Results Seven days after modeling, the NSS was higher in the high-fat group than in the control group (t=10.053, P<; 0.05), and no significant difference was found in the infarct volume between two groups (t=6.872, P>; 0.05). The expression of Sirt1 (t=8.462, P<; 0.05) and AIF (t=8.471, P<; 0.05) was higher, and the expression of Wnt (t=17.752, P<; 0.01) was lower in the high-fat group than in the control group.Conclusion Obesity could affect the recovery of mice with MCAO, which may related with impaired Wnt signaling.

Key words: ischemic brain injury, obesity, mice

中图分类号:

R743.3

张继红, 马存根. 高脂饮食诱导肥胖对小鼠缺血性脑损伤的影响[J]. 《中国康复理论与实践》, 2018, 24(10): 1146-1150.

ZHANG Ji-hong, MA Cun-gen. Effect of Obesity Induced by High-fat Diet on Ischemic Brain Injury in Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(10): 1146-1150.

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