《中国康复理论与实践》

《中国康复理论与实践》 ›› 2019, Vol. 25 ›› Issue (12): 1425-1433.doi: 10.3969/j.issn.1006-9771.2019.00.014

• 基础研究 • 上一篇    下一篇

电针夹脊穴和足阳明胃经穴对脊髓损伤大鼠下肢神经功能恢复和突触素I的影响比较

吴俏锋1, 周宾宾2, 魏卫兵1, 覃镜羽1, 冯振奋1, 李建男1   

  1. 1.广西中医药大学，广西南宁市 530001
    2.广西中医药大学第一附属医院，广西南宁市 530023
  • 收稿日期:2019-07-01 修回日期:2019-07-23 出版日期:2019-12-25 发布日期:2019-12-26
  • 通讯作者: 周宾宾，E-mail: zbb8321@foxmail.com E-mail:zbb8321@foxmail.com
  • 作者简介:吴俏锋(1993-)，男，汉族，广东恩平市人，硕士研究生，主要研究方向：颈椎病的诊治与研究。
  • 基金资助:
    1.国家自然科学基金项目(No. 84160746)；2.广西自然科学基金项目(No. 2016GXNSFAA380311)

Comparison of Electroacupuncture at Jiaji (EX-B2) Points and Stomach Meridian Foot-Yang Ming Points on Recovery of Lower Limb Nerve Function and Synapsin I Expression in Spinal Cord Injury Rats

WU Qiao-feng1, ZHOU Bin-bin2, WEI Wei-bing1, QIN Jing-yu1, FENG Zhen-fen1, LI Jian-nan1   

  1. 1.Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, China
    2.the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, China
  • Received:2019-07-01 Revised:2019-07-23 Published:2019-12-25 Online:2019-12-26
  • Contact: ZHOU Bin-bin, E-mail: zbb8321@foxmail.com E-mail:zbb8321@foxmail.com
  • Supported by:
    Supported by National Natural Science Foundation of China (No. 84160746) and Guangxi Natural Science Foundation (No. 2016GXNSFAA380311)

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摘要： 目的 观察电针夹脊穴和足阳明胃经穴对脊髓损伤后大鼠神经功能的影响及其损伤局部脊髓突触素I表达量的改变，探究其作用及分子机制。 方法 采用缺血损伤型方法建立60只Sprague-Dawley大鼠脊髓损伤模型，随机分为对照组、夹脊穴电针组(夹脊组)和足阳明胃经电针组(阳明组)，每组20只。于术后第3天开始对夹脊组和阳明组进行电针干预，对照组不做处理。每天进行BBB评分。各组分别在干预第1、2、3、4、5周结束时处死4只大鼠，HE染色观察损伤脊髓形态，免疫组化染色检测Synapsin I蛋白表达，RT-qPCR法测定Synapsin I mRNA水平，Western blotting检测Synapsin I蛋白含量。 结果 夹脊组和阳明组术后1~5周BBB评分均高于对照组(P < 0.05)；阳明组1~3周BBB评分高于夹脊组(P < 0.05)，4~5周两组间比较无显著性差异(P > 0.05)。夹脊组1周时Synapsin I蛋白免疫组化评分(IHS)高于对照组(P < 0.05)，阳明组1~4周均高于对照组(P < 0.05)；阳明组3~4周时Synapsin I蛋白IHS高于夹脊组(P < 0.05)。对照组Synapsin I mRNA和蛋白表达均随着时间的延长呈现先升高后降低的趋势；夹脊组1周时Synapsin I mRNA和蛋白表达均高于对照组(P < 0.05)，阳明组1~4周均高于对照组(P < 0.05)；阳明组Synapsin I mRNA(3周时)、Synapsin I蛋白(2~3周时)表达均高于夹脊组(P < 0.05)。 结论 电针夹脊穴及足阳明胃经穴对脊髓损伤大鼠神经功能的恢复均有促进作用，可能与损伤部分脊髓Synapsin I水平上调有关。

关键词: 脊髓损伤, 电针, 夹脊穴, 足阳明胃经穴, 神经功能, 突触素I, 大鼠

Abstract: Objective To investigate the effect of electroacupuncture at Jiaji (EX-B2) points and Stomach Meridian Foot-Yang Ming points on the neurological function and the expression of local Synapsin I in rats after spinal cord injury, and to explore its molecular mechanism. Methods A total of 60 Sprague-Dawley rats were established spinal cord injury model and they were randomly divided into normal control group, Jiaji group and Yang Ming group, with 20 rats in each group. On the third day after operation, Jiaji group and Yang Ming group were treated with electroacupuncture while the normal control group was not. Their neurological function was assessed with BBB every day. Four rats in each group were sacrificed at the end of the 1st, 2nd, 3rd, 4th and 5th week during the intervention period. HE staining was used to observe the morphology of spinal cord. Immumohistochemical staining was used to detect the expression of Synapsin I protein. Reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting was used to detect the content of of Synapsin I mRNA and protein. Results The BBB scores were higher in Jiaji group and Yang Ming group than in the normal control group at all the time points (P < 0.05), and were higher in Yang Ming group than in Jiaji group from one to three weeks (P < 0.05). The immunohistochemical scores of Synapsin I protein were higher in Jiaji group than in the normal control group in the first week (P < 0.05), and were higher in Yang Ming group than in the normal control group from one to four weeks (P < 0.05). The immunohistochemical scores of Synapsin I protein were higher in Yang Ming group than in Jiaji group from three to four weeks (P < 0.05). The expression of Synapsin I mRNA and protein first increased and then decreased in the normal control group; which was higher in Jiaji group than in the normal control group in the first week (P < 0.05) and was higher in Yang Ming group than in the normal control group from one to four weeks (P < 0.05). The Synapsin I mRNA expression in the third week and the Synapsin I protein expression from two to three weeks was higher in Yang Ming group than in Jiaji group (P < 0.05). Conclusion Electroacupuncture at both Jiaji points and Stomach Meridian Foot-Yang Ming points promote the recovery of nerve function in rats with spinal cord injury. It may be related to the elevated level of Synapsin I in the damaged parts of the spinal cord.

Key words: spinal cord injury, electroacupuncture, Jiaji points, Stomach Meridian Foot-Yang Ming points, neurological function, Synapsin I, rats

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