电针对SAMP8小鼠血脑屏障的影响

doi:10.3969/j.issn.1006-9771.2019.02.006

《中国康复理论与实践》 ›› 2019, Vol. 25 ›› Issue (2): 156-161.doi: 10.3969/j.issn.1006-9771.2019.02.006

电针对SAMP8小鼠血脑屏障的影响

杨之雪¹,唐成林¹,李小宏²,朱正威¹,黄思琴¹, 代妮¹,吴梦佳¹,邱丽¹,安荟羽¹

1. 重庆医科大学中医药学院，重庆市 400016
2. 重庆医科大学附属第一医院康复医学科，重庆市 400016

收稿日期:2018-11-27 修回日期:2018-12-29 出版日期:2019-02-25 发布日期:2019-04-01
通讯作者: 唐成林、李小宏。E-mail: cytcl996@163.com(唐成林); 25089598@qq.com(李小宏)
作者简介:杨之雪(1993-)，女，汉族，重庆市人，硕士研究生，主要研究方向：针灸推拿机制研究。
基金资助:
1.国家自然科学基金青年科学基金项目(No. 81603689)；2.国家自然科学基金项目(No. 81273870)；3.重庆市卫计委/重庆市科委中医药重点科研项目(No. ZY201801007)

Effect of Electroacupuncture on Blood-brain Barrier in SAMP8 Mice

YANG Zhi-xue¹, TANG Cheng-lin¹, LI Xiao-hong², ZHU Zheng-wei¹, HUANG Si-qin¹, DAI Ni¹, WU Meng-jia¹, QIU Li¹, AN Hui-yu¹

1. College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, China
2. Department of Rehabilitation, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Received:2018-11-27 Revised:2018-12-29 Published:2019-02-25 Online:2019-04-01
Contact: TANG Cheng-lin, LI Xiao-hong. E-mail: cytcl996@163.com (TANG Cheng-lin); 25089598@qq.com (LI Xiao-hong)
Supported by:
National Natural Science Foundation of China (Youth) (No. 81603689), National Natural Science Foundation of China (No. 81273870) and Chongqing Health and Family Planning Committee/Chongqing Science and Technology Committee Key Research Project for Traditional Chinese Medicine (No. ZY201801007)

摘要/Abstract

摘要： 目的研究电针通过对快速老化痴呆模型SAMP8小鼠血脑屏障的调控，以期加强药物入脑。方法 30周龄雄性SAMP8小鼠随机分为模型组(n = 7)、电针组(n = 7)、药物组(n = 7)和针药组(n = 7)，以同源抗快速老化小鼠SAMR1作为对照组(n = 7)。电针组和针药组电针印堂、百会；药物组和针药组予盐酸多奈哌齐灌胃；对照组和模型组常规抓取。共4周。电镜观察血脑屏障紧密连接开放程度和基底膜厚度；定量聚合酶链式反应检测海马区紧密连接蛋白ZO-1、Claudin-5和Occludin mRNA的表达，免疫组化检测海马区乙酰胆碱酯酶(AChE)的含量。结果对照组、电针组和针药组紧密连接以及基底膜结构均优于模型组和药物组；针药组AChE表达最低，以下依次是药物组和对照组、电针组、模型组(P < 0.001)；电针组和针药组ZO-1、Claudin-5和Occludin mRNA表达水平高于药物组(P < 0.01)。结论电针能够改善SAMP8小鼠血脑屏障结构，可能与调节紧密连接蛋白ZO-1、Claudin-5和Occludin的表达有关。

关键词: 阿尔茨海默病, 电针, 血脑屏障, 小鼠

Abstract: Objective To investigate the effects of electroacupuncture on blood-brain barrier to promote intake of drugs into brain of SAMP8 mice. Methods The male SAMP8 mice (30-week-old) were randomly divided into model group (n = 7), drug group (n = 7), acupuncture group (n = 7) and combined group (n = 7). Other SAMR1 mice were as control group (n = 7). The acupuncture group and the combined group accepted electroacupuncture at Baihui (DU20) and Yintang (EX-HN3), the drug group and the combined group accepted Donepezil, for four weeks. They were observed hippocampus tight junction (TJ) under transmission electron microscope. The activities of acetylcholinesterase (AChE) in hippocampus were detected with immunohistochemistry. The mRNA expression of ZO-1, Claudin-5 and Occludin was detected with real-time quantitative polymerase chain reaction. Results TJ and basement membrane in the control group, the acupuncture group and the combined group were better than those in the model group and the drug group. AChE was the most in the combined group, and then the drug group and the control group, the acupuncture group and the model group (P < 0.001). The expression of mRNA of ZO-1, Claudin-5 and Occludin was more in the acupuncture group and the combined group than in the drug group (P < 0.01). Conclusion Electroacupuncture could ameliorate blood-brain barrier disruption and promote drug to enter the brain in SAMP8 mice, which may relate to the adjustion of ZO-1, Claudin-5 and Occludin.

Key words: Alzheimer's disease, electroacupuncture, blood-brain barrier, mice

中图分类号:

R749.1

杨之雪, 唐成林, 李小宏, 朱正威, 黄思琴, 代妮, 吴梦佳, 邱丽, 安荟羽. 电针对SAMP8小鼠血脑屏障的影响[J]. 《中国康复理论与实践》, 2019, 25(2): 156-161.

YANG Zhi-xue, TANG Cheng-lin, LI Xiao-hong, ZHU Zheng-wei, HUANG Si-qin, DAI Ni, WU Meng-jia, QIU Li, AN Hui-yu. Effect of Electroacupuncture on Blood-brain Barrier in SAMP8 Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2019, 25(2): 156-161.

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电针对SAMP8小鼠血脑屏障的影响

Effect of Electroacupuncture on Blood-brain Barrier in SAMP8 Mice

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