《中国康复理论与实践》 ›› 2019, Vol. 25 ›› Issue (3): 302-306.doi: 10.3969/j.issn.1006-9771.2019.03.011

• 基础研究 • 上一篇    下一篇

运动预处理对大鼠脑缺血再灌注损伤后血脑屏障通透性的影响

梁碧莹1, 朱路文2, 唐强2, 叶涛3, 李宏玉2, 李保龙2, 阮野2   

  1. 1.黑龙江中医药大学,黑龙江哈尔滨市 150040
    2.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001
    3.贵阳中医学院第一附属医院,贵州贵阳市 550000
  • 收稿日期:2018-06-24 修回日期:2018-10-17 出版日期:2019-03-25 发布日期:2019-04-02
  • 通讯作者: 朱路文,E-mail: zhuluwen2009@126.com
  • 作者简介:梁碧莹(1993-),女,满族,黑龙江哈尔滨市人,博士研究生,主要研究方向:运动预处理预防脑血管病。通讯作者:朱路文(1983-),男,山东邹城市人,博士,博士后,副主任医师,主要研究方向:运动预处理的脑保护机制。
  • 基金资助:
    1.中国博士后科学基金项目(No. 2017M611411);2.黑龙江省博士后基金项目(No. LBH-Z16195);3.哈尔滨市科技创新人才研究专项资金项目(No. RC2017QN003087)

Effects of Exercise Preconditioning on Blood-brain Barrier Permeability in Rats after Cerebral Ischemia-reperfusion Injury

LIANG Bi-ying1, ZHU Lu-wen2, TANG Qiang2, YE Tao3, LI Hong-yu2, LI Bao-long2, RUAN Ye2   

  1. 1.Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China
    2.The Second Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China
    3.The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang, Guizhou 550000, China
  • Received:2018-06-24 Revised:2018-10-17 Published:2019-03-25 Online:2019-04-02
  • Contact: ZHU Lu-wen, E-mail: zhuluwen2009@126.com
  • Supported by:
    China Postdoctoral Science Foundation (No. 2017M611411), Heilongjiang Postdoctoral Science Foundation (No. LBH-Z16195) and Harbin Fund for Young Professional Training (No. RC2017QN003087)

摘要: 目的 观察运动预处理对大鼠脑缺血再灌注损伤后血脑屏障通透性,以及连接蛋白43 (Cx43)和泛连接蛋白1 (Panx1)的影响。方法 54只雄性Sprague-Dawley大鼠随机分为假手术组、模型组和运动预处理组,每组18只。造模前,运动预处理组行跑台训练3周。采用改良Koizumi线栓法闭塞对模型组和运动预处理组大脑中动脉,再灌注24 h后,采用改良神经功能缺损评分(mNSS)评估神经功能;伊文思蓝渗透法观察血脑屏障通透性;Western blotting和免疫组化检测缺血侧脑组织Cx43和Panx1蛋白表达。结果 与模型组比较,运动预处理组mNSS评分降低(P < 0.05),伊文思蓝含量以及Cx43和Panx1蛋白表达量下降(P < 0.05),Cx43和Panx1阳性面积率降低(P < 0.05)。结论 运功预处理可改善脑缺血再灌注大鼠血脑屏障通透性,下调Cx43蛋白和Panx1蛋白表达,具有脑保护作用。

关键词: 脑缺血再灌注损伤, 运动预处理, 血脑屏障, 连接蛋白43, 泛连接蛋白1, 大鼠

Abstract: Objective To observe the effects of exercise preconditioning on blood-brain barrier (BBB) permeability, and connexin 43 (Cx43) and pannexin 1 (Panx1) protein after cerebral ischemia-reperfusion injury in rats. Methods Fifty-four male Sprague-Dawley rats were randomly divided into sham group (n = 18), model group (n = 18) and exercise preconditioning group (n = 18). The exercise preconditioning group was trained with treadmill for three weeks before modeling. The middle cerebral arteries were occluded in the model group and the exercise preconditioning group using the modified Koizumi suture. After reperfusion of 24 hours, the rats were assessed with modified Neurological Severity Score (mNSS). The permeability of BBB was observed with Evans blue (EB). The expression of Cx43 and Panx1 was detected with Western blotting and immunohistochemistry in the ischemic tissues. Results Compared with the model group, the mNSS score decreased in the exercise preconditioning group (P < 0.05), while the Evans blue content and the expression of Cx43 and Panx1 decreased (P < 0.05), as well as the the positive areas of Cx43 and Panx1 (P < 0.05). Conclusion Exercise preconditioning can improve the permeability of BBB in cerebral ischemia-reperfusion rats, which may associate with down-regulation of Cx43 and Panx1, to protect brain from injury.

Key words: cerebral ischemia-reperfusion injury, exercise preconditioning, blood-brain barrier, connexin 43, pannexin 1, rats

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