大鼠脊髓损伤后脊髓组织中上调差异基因的生物信息学分析

doi:10.3969/j.issn.1006-9771.2019.06.001

《中国康复理论与实践》 ›› 2019, Vol. 25 ›› Issue (6): 621-629.doi: 10.3969/j.issn.1006-9771.2019.06.001

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大鼠脊髓损伤后脊髓组织中上调差异基因的生物信息学分析

钟占琼¹, 薛清彩¹, 易晓红¹, 黄聪¹, 于海艳¹, 刘伟伟¹, 陈继兰¹, 夏军¹, 张晓²

1.成都中医药大学基础医学院，四川成都市 611137;
2.成都医学院基础医学院，四川成都市610500

收稿日期:2019-03-19 修回日期:2019-05-07 发布日期:2019-06-25
通讯作者: 张晓，E-mail: zhangxiao1985007@163.com
作者简介:钟占琼(1988-)，女，汉族，四川高县人，硕士研究生，实验师，主要研究方向：神经损伤与修复。
基金资助:
成都中医药大学科技发展基金项目(No. ZRQN1720)

Bioinformatics Analysis of Up-regulated Differential Genes in Rats post Spinal Cord Injury

ZHONG Zhan-qiong¹, XUE Qing-cai¹, YI Xiao-hong¹, HUANG Cong¹, YU Hai-yan¹, LIU Wei-wei¹, CHEN Ji-lan¹, XIA Jun¹, ZHANG Xiao²

1.School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China;
2.Preclinical Medicine of Chengdu Medical College, Chengdu, Sichuan 610500, China

Received:2019-03-19 Revised:2019-05-07 Online:2019-06-25
Contact: ZHANG Xiao, E-mail: zhangxiao1985007@163.com
Supported by:
Scientific Development Fund Projects of Chengdu University of TCM (No. ZRQN1720)

摘要/Abstract

摘要： 目的探究大鼠脊髓损伤后脊髓组织中差异表达的上调基因及功能。方法采用改良Allen法制备雌性Sprague-Dawley大鼠脊髓损伤模型，应用基因芯片技术检测在大鼠脊髓损伤后脊髓中差异基因的变达，运用基因本体论和基因组百科全书数据库分析差异上调基因、功能定位与通路。结果脊髓组织总核糖核酸质量检测结果合格；基因芯片检测结果显示上调差异基因1874个，下调差异基因2348个；生物信息学分析上调差异基因的生物学过程、细胞组分和分子功能，涉及凋亡、免疫反应、炎症等，通路分析主要涉及磷脂酰肌醇三激酶-蛋白激酶B信号通路和Toll样受体信号通路等。结论脊髓损伤后，上调差异表达基因可能参与脊髓损伤后的继发性反应，如炎症、免疫应答、缺氧等，继而影响脊髓损伤后的运动功能和感觉功能。

关键词: 脊髓损伤, 上调基因, 生物信息学

Abstract: Objective To investigate the differential expression and gene functions of up-regulated genes in rats with spinal cord injury.Methods Female Sprague-Dawley rats' model of spinal cord injury was established with the modified Allen's method. Gene chip technology was used to detect the variation of differentially expressed genes in the spinal cord after spinal cord injury in rats. The differences in genes, functional localization and pathways were analyzed with gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway database.Results The results of total RNA quality in spinal cord segment were qualified. Gene chip results showed that there were 1874 differentially up-regulated genes and 2348 differentially down-regulated genes. Bioinformatics was used to analyze differentially up-regulated genes in terms of biological processes, cellular components, and molecular functions. The differentially up-regulated genes were involving apoptosis, immune response, inflammation, etc., pathway analysis mainly showed the differentially up-regulated genes involved phosphoinositide 3-kinase protein kinase B signaling pathway and Toll-like receptor signaling pathways.Conclusion Differentially up-regulated genes may be involved in secondary reactions following spinal cord injury, such as inflammation, immune response and hypoxia, and then further affect motor function and sensory function.

Key words: spinal cord injury, up-regulated genes, bioinformatics

中图分类号:

R651.2

钟占琼, 薛清彩, 易晓红, 黄聪, 于海艳, 刘伟伟, 陈继兰, 夏军, 张晓. 大鼠脊髓损伤后脊髓组织中上调差异基因的生物信息学分析[J]. 《中国康复理论与实践》, 2019, 25(6): 621-629.

ZHONG Zhan-qiong, XUE Qing-cai, YI Xiao-hong, HUANG Cong, YU Hai-yan, LIU Wei-wei, CHEN Ji-lan, XIA Jun, ZHANG Xiao. Bioinformatics Analysis of Up-regulated Differential Genes in Rats post Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2019, 25(6): 621-629.

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大鼠脊髓损伤后脊髓组织中上调差异基因的生物信息学分析

Bioinformatics Analysis of Up-regulated Differential Genes in Rats post Spinal Cord Injury

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