急性创伤性完全性脊髓损伤患者肠道菌群多样性研究

doi:10.3969/j.issn.1006-9771.2019.12.014

《中国康复理论与实践》 ›› 2019, Vol. 25 ›› Issue (12): 1450-1457.doi: 10.3969/j.issn.1006-9771.2019.12.014

急性创伤性完全性脊髓损伤患者肠道菌群多样性研究

张洁^1,2,3,4, 李建军^1,2,3,4, 张超^1,2,3,4, 杨德刚^1,2,3,4, 杨明亮^1,2,3,4, 杜良杰^1,2,3,4, 高峰^1,2,3,4, 申敏鑫^1,2,3,4

1.首都医科大学康复医学院，北京市 100068
2.中国康复研究中心北京博爱医院脊柱脊髓神经功能重建科，北京市 100068
3.北京脑重大疾病研究院神经损伤与修复研究所，北京市 100068
4.北京市神经损伤与康复重点实验室，北京市 100068

收稿日期:2019-10-23 修回日期:2019-11-03 出版日期:2019-12-25 发布日期:2019-12-26
通讯作者: 李建军，E-mail: crrc100@163.com E-mail:crrc100@163.com
作者简介:张洁(1992-)，女，汉族，河北深泽县人，硕士研究生，主要研究方向：脊柱脊髓损伤的康复与治疗。
基金资助:
北京市科技重大专项课题(No. Z171100001017076)

Diversity of Gut Microbiota in Patients with Acute Traumatic Complete Spinal Cord Injury

ZHANG Jie^1,2,3,4, LI Jian-jun^1,2,3,4, ZHANG Chao^1,2,3,4, YANG De-gang^1,2,3,4, YANG Ming-liang^1,2,3,4, DU Liang-jie^1,2,3,4, GAO Feng^1,2,3,4, SHEN Min-xin^1,2,3,4

1.Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China
2.Department of Spinal and Neural Function Reconstruction, China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing 100068, China
3.Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100068, China
4.Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing 100068, China

Received:2019-10-23 Revised:2019-11-03 Published:2019-12-25 Online:2019-12-26
Contact: LI Jian-jun, E-mail: crrc100@163.com E-mail:crrc100@163.com
Supported by:
Supported by Beijing Major Science and Technology Project (No. Z171100001017076)

摘要/Abstract

摘要： 目的对比分析急性创伤性完全性脊髓损伤患者和健康人的肠道菌群及生化指标。方法 2017年5月至2018年5月，收集44例急性创伤性完全性脊髓损伤患者(患者组)和33例健康对照者(对照组)的临床数据和新鲜血、尿、粪便标本。对肠道微生物16S rRNA基因的V3-V4区域进行测序分析。结果与对照组比较，患者组肠道菌群的丰度增高，结构组成有差异；患者组Bacteroidetes表达减少(P < 0.05)，Actinobacteria、Proteobacteria、Synergistetes、Saccharibacteria和Cyanobacteria表达增多(P < 0.05)。血清葡萄糖、低密度脂蛋白、甘油三酯和总胆固醇较对照组增高(P < 0.05)，这些标记物升高与肠道微生物群落改变存在显著相关性(P < 0.05)。结论急性创伤性完全性脊髓损伤患者存在肠道菌群紊乱，这些菌群的变化与血清生物标志物的升高相关。

关键词: 脊髓损伤, 肠道菌群, 菌群多样性, 血清标志物

Abstract: Objective To Compare and analyze the gut microbiota and biochemical indexes between patients with acute traumatic complete spinal cord injury and healthy subjects. Methods From May, 2017 to May, 2018, a total of 44 patients with acute traumatic complete spinal cord injury (patient group) and 33 healthy controls (control group) were included. The clinical data and fresh blood, urine and fecal samples of the two groups were collected. The V3-V4 region of 16S rRNA gene was sequenced and analyzed. Results The abundance of gut microbiota was higher in the patient group than in the control group, and the structural composition was different. Compared with the control group, the expression of Bacteroidetes decreased (P < 0.05), and the expression of Actinobacteria, Proteobacteria, Synergistetes, Saccharibacteria and Cyanobacteria increased in the patient group (P < 0.05). The serum glucose, low density lipoprotein, triglyceride and total cholesterol were significantly higher in the patient group than in the control group (P < 0 05). There was a significant correlation between these elevated markers and intestinal microbial community structure (P < 0.05). Conclusion There is gut microbiota dysbiosis in patients with acute traumatic complete spinal cord injury, and the changes of the microbiota are related to the elevation of some serum biomarkers.

Key words: spinal cord injury, gut microbiota, microbial diversity, serum biomarker

中图分类号:

R651.2

张洁, 李建军, 张超, 杨德刚, 杨明亮, 杜良杰, 高峰, 申敏鑫. 急性创伤性完全性脊髓损伤患者肠道菌群多样性研究[J]. 《中国康复理论与实践》, 2019, 25(12): 1450-1457.

ZHANG Jie, LI Jian-jun, ZHANG Chao, YANG De-gang, YANG Ming-liang, DU Liang-jie, GAO Feng, SHEN Min-xin. Diversity of Gut Microbiota in Patients with Acute Traumatic Complete Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2019, 25(12): 1450-1457.

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急性创伤性完全性脊髓损伤患者肠道菌群多样性研究

Diversity of Gut Microbiota in Patients with Acute Traumatic Complete Spinal Cord Injury

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