大鼠骨骼肌急性损伤后早期运动训练和按摩对肌卫星细胞增殖相关因子的影响

doi:10.3969/j.issn.1006-9771.2020.01.008

《中国康复理论与实践》 ›› 2020, Vol. 26 ›› Issue (1): 49-54.doi: 10.3969/j.issn.1006-9771.2020.01.008

大鼠骨骼肌急性损伤后早期运动训练和按摩对肌卫星细胞增殖相关因子的影响

贺舟¹,常青²(),唐成林³,朱正威³

1.重庆医科大学附属康复医院,重庆市 400016
2.重庆医科大学体育医学院,重庆市 400016
3.重庆医科大学中医药学院,重庆市 400016

收稿日期:2019-08-16 修回日期:2019-09-09 出版日期:2020-01-25 发布日期:2020-02-07
通讯作者: 常青 E-mail:651733149@qq.com
作者简介:贺舟(1986-),男,土家族,湖北恩施市人,硕士研究生,主要研究方向：运动损伤。
基金资助:
重庆医科大学哲学社会科学专项重点项目(201708)

Effect of Early Treadmill Exercise and Massage on Activative Factors of Muscle Satellite Cells after Acute Injury of Skeletal Muscle in Rats

HE Zhou¹,CHANG Qing²(),TANG Cheng-lin³,ZHU Zheng-wei³

1. The Affiliated Rehabilitation Hosptial of Chongqing Medical University, Chongqing 400016, China
2. Sports Medical College, Chongqing Medical University, Chongqing 400016, China
3. College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, China

Received:2019-08-16 Revised:2019-09-09 Published:2020-01-25 Online:2020-02-07
Contact: CHANG Qing E-mail:651733149@qq.com
Supported by:
Chongqing Medical University Key Project(201708)

摘要/Abstract

摘要：

目的探讨骨骼肌急性损伤后24 h内介入运动和按摩对肌卫星细胞激活关键因子胰岛素样生长因子(IGF)-1,以及肌卫星细胞增殖通路丝裂原活化蛋白激酶(MAPK)/丝裂原活化蛋白激酶激酶(MEK)/细胞外调节蛋白激酶(ERK)1/2的影响。
方法 40只SPF级成年雄性Sprague-Dawley大鼠随机分为对照组(A组, n = 8)、模型组(B组, n = 8)、按摩组(C组, n = 8)、跑台运动组(D组, n = 8)和按摩联合跑台运动组(E组, n = 8)。A组不做任何处理,B、C、D、E组用改良打击器制备大鼠腓肠肌急性损伤模型;B组不予任何干预,自然恢复;C组于造模后24 h内介入按摩;D组在造模后24 h内介入跑台运动训练;E组在造模成功后24 h内介入跑台运动和按摩。造模24 h,取动物腓肠肌,HE染色观察其形态;逆转录聚合酶链反应(RT-PCR)检测成肌分化抗原(MyoD1)和肌细胞生成素(MyoG) mRNA,Western blotting检测IGF-1、p-MAPK、p-MEK和p-ERK1/2的表达量。
结果 C组、D组和E组MyoD1 mRNA表达量均高于B组,C组最高;C组、D组和E组MyoG mRNA表达量均低于B组,E组最高(P < 0.05);C组、D组、E组p-MAPK、p-MEK、p-EPK1/2表达量均高于B组( P < 0.05),D组最高( P < 0.05);C组IGF-1较B组升高( P < 0.05),D组较B组降低( P < 0.05)。
结论在骨骼肌急性损伤后24 h内介入跑台运动和按摩,能各有侧重地通过不同途径激活肌卫星细胞。

关键词: 骨骼肌急性损伤, 跑台运动, 按摩, 肌卫星细胞, 大鼠

Abstract:

Objective To study the effect of treadmill exercise and massage shortly after acute injury on expression of key growth factor in muscle satellite cells activation, insulin-like growth factor-1 (IGF-1), and mitogen-activated protein kinase (MAPK) / mitogen-activated protein kinase kinase (MEK) / extracellular regulated protein kinases (ERK)1/2 signaling pathways in muscle satellite cell in rats.
Methods A total of 40 SPF male adult Sprague-Dawley rats were randomly divided into groups A (n = 8), B (n = 8), C (n = 8), D (n = 8) and E (n = 8). Group A did not receive any treatment, while the other rats were contused the gastrocnemius muscle with self-made impactor. Group B received no intervention, groups C and D received massage and treadmill exercise shortly after injury, respectively, while group E received both treadmill exercise and massage shortly after injury. As the model was established, samples of gastrocnemius were obtained from all the rats 24 hours after injury, and observed under HE staining, detected the expression of MyoD1 and MyoG mRNA with reverse transcription polymerase chain reaction (RT-PCR), and expression of IGF-1, p-MAPK, p-MEK and p-ERKI/2 protein with Western blotting.
Results The expression of MyoD1 mRNA was more in groups C, D and E than in group B, which was the most in group C; less expression of MyoG mRNA, which was the most in group E (P < 0.05). The expression of p-MAPK, p-MEK and p-EPK1/2 was more in groups C, D and E than in group B, which was the most in group D ( P < 0.05). The expression of IGF-1 increased in group C compared with that in group B ( P < 0.05), and it decreased in group D ( P < 0.05).
Conclusion Early intervention of treadmill exercise and massage may promote the activation of muscle satellite cells in different ways.

Key words: acute skeletal muscle injury, treadmill exercise, massage, muscle satellite cells, rats

中图分类号:

R685.5

贺舟,常青,唐成林,朱正威. 大鼠骨骼肌急性损伤后早期运动训练和按摩对肌卫星细胞增殖相关因子的影响[J]. 《中国康复理论与实践》, 2020, 26(1): 49-54.

HE Zhou,CHANG Qing,TANG Cheng-lin,ZHU Zheng-wei. Effect of Early Treadmill Exercise and Massage on Activative Factors of Muscle Satellite Cells after Acute Injury of Skeletal Muscle in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(1): 49-54.

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组别	n	IGF-1	p-MAPK	p-MEK	P-ERK1/2
A组	8	1.089±0.036	0.678±0.040	0.404±0.024	0.370±0.014
B组	8	0.832±0.057^a	0.439±0.056^a	0.343±0.003^a	0.274±0.016^a
C组	8	0.938±0.065^a,b	0.669±0.015^b	0.619±0.015^a,b	0.633±0.043^a,b
D组	8	0.759±0.045^a,b,c	1.067±0.037^a,b,c	0.917±0.019^a,b,c	1.022±0.051^a,b,c
E组	8	0.844±0.036^a,c,d	0.749±0.04^a,b,c,d	0.506±0.035^a,b,c,d	0.855±0.057^a,b,c,d
F值		53.357	256.328	847.419	493.807
P值		< 0.001	< 0.001	< 0.001	< 0.001

组别	n	MyoD1	MyoG
A组	8	0.417±0.010	0.175±0.006
B组	8	0.445±0.007^a	0.234±0.007^a
C组	8	0.493±0.008^a,b	0.208±0.003^a,b
D组	8	0.480±0.011^a,b,c	0.216±0.002^a,b,c
E组	8	0.464±0.009^a,b,c,d	0.222±0.005^a,b,c,d
F值		87.458	169.181
P值		< 0.001	< 0.001

大鼠骨骼肌急性损伤后早期运动训练和按摩对肌卫星细胞增殖相关因子的影响

Effect of Early Treadmill Exercise and Massage on Activative Factors of Muscle Satellite Cells after Acute Injury of Skeletal Muscle in Rats

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