水通道蛋白过表达对脊髓损伤大鼠便秘的影响

doi:10.3969/j.issn.1006-9771.2020.02.008

《中国康复理论与实践》 ›› 2020, Vol. 26 ›› Issue (2): 181-188.doi: 10.3969/j.issn.1006-9771.2020.02.008

水通道蛋白过表达对脊髓损伤大鼠便秘的影响

徐珮珮^1,^2,^3,⁴,杨明亮^1,^2,^3,⁴(),刘长彬⁵,李雅静^1,^2,^3,⁴,刘梓桐^1,^2,^3,⁴,李建军^1,^2,^3,⁴

1.首都医科大学康复医学院,北京市 100068
2.中国康复研究中心北京博爱医院脊柱脊髓神经功能重建科,北京市 100068
3.北京脑重大疾病研究院神经损伤与修复研究所,北京市 100068
4.北京市神经损伤与康复重点实验室,北京市 100068
5.首都医科大学附属北京天坛医院康复医学科,北京市 100070

收稿日期:2019-12-02 修回日期:2020-01-03 出版日期:2020-02-25 发布日期:2020-03-19
通讯作者: 杨明亮 E-mail:chinayml3@163.com
作者简介:徐珮珮(1995-),女,汉族,安徽芜湖市人,硕士研究生,主要研究方向:脊柱脊髓损伤后并发症治疗。
基金资助:
1.北京市自然科学基金项目(7192238);2.中国康复研究中心基础科研基金项目(2018ZX-9)

Aquaporin Over-expression for Constipation in Rats with Spinal Cord Injury

XU Pei-pei^1,^2,^3,⁴,YANG Ming-liang^1,^2,^3,⁴(),LIU Chang-bin⁵,LI Ya-jing^1,^2,^3,⁴,LIU Zi-tong^1,^2,^3,⁴,LI Jian-jun^1,^2,^3,⁴

1. Capital Medical University School of Rehabilitation Medicine, Beijing, 100068, China
2. Department of Spinal and Neural Function Reconstruction, Beijing Bo'ai Hospital, China Rehabilitation Research Center, Beijing 100068, China
3. Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, 100068, China
4. Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, 100068, China
5. Department of Rehabilitation Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China

Received:2019-12-02 Revised:2020-01-03 Published:2020-02-25 Online:2020-03-19
Contact: YANG Ming-liang E-mail:chinayml3@163.com
Supported by:
Beijing Municipal Natural Science Foundation(7192238);China Rehabilitation Research Center Basic Scientific Research Foundation(2018ZX-9)

摘要/Abstract

摘要：

目的探讨脊髓损伤大鼠结肠水通道蛋白(AQPs)的表达及其与粪便含水率的关系。方法将48只雌性Sprague-Dawley大鼠分为对照组(n = 24)和脊髓损伤组(n = 24)。脊髓损伤组行T₈横断脊髓损伤,对照组仅行椎板切除术。于造模前,造模后第1、3、7、14、28天,采用Basso-Beattie-Bresnahan (BBB)评分评价后肢运动功能;于术前,术后第3、14、28天检测粪便含水率;于术后第3、14、28天取结肠标本,免疫组化检测AQP1、AQP3和AQP4的表达。结果脊髓损伤组术后BBB评分(t > 69.230, P< 0.001)和粪便含水率(t > 5.814, P< 0.001)均显著低于对照组。术后第3、14、28天,脊髓损伤组结肠AQP1、AQP3和AQP4表达均明显高于对照组(|t|> 5.165,P < 0.01)。脊髓损伤后AQPs表达与粪便含水率呈显著负相关( r = -0791~-0.730, P< 0.001)。结论脊髓损伤后大鼠结肠AQPs表达上调,可能与脊髓损伤后结肠过度吸收水分有关。

关键词: 脊髓损伤, 肠道功能障碍, 水通道蛋白, 便秘, 结肠, 粪便含水率, 大鼠

Abstract:

Objective To investigate the relation between aquaporins (AQPs) and fecal water content in rats with spinal cord injury.Methods A total of 48 female Sprague-Dawley rats were divided into control group (n= 24) and spinal cord injury group (SCI group, n = 24). SCI group underwent transection at T₈, while the control group was only subjected to laminectomy. Posterior limb function was assessed by Basso-Beattie-Bresnahan (BBB) score before modeling and on the 1st, 3rd, 7th, 14th and 28th day after SCI. Fecal water content was measured before modeling and on the 3rd, 14th and 28th day after SCI. Colon specimens were collected to detect the expression of AQP1, AQP3 and AQP4 by immunohistochemistry on the 3rd, 14th and 28th day after SCI.Results The BBB score was significantly lower in SCI group than in the control group (t > 69.230, P< 0.001) after SCI, as well as the fecal water content (t > 5.814, P< 0.001). The expression of AQP1, AQP3, and AQP4 in the colon was higher in SCI group than in the control group (|t|> 5.165,P < 0.01) on the 3rd, 14th, and 28th day after SCI. The expression of AQPs negatively correlated with fecal water content ( r = -0791~-0.730, P< 0.001).Conclusion The expression of AQPs in the colon of rats after SCI was up-regulated, which was correlated with excessive water absorption after SCI.

Key words: spinal cord injury, bowel dysfunction, aquaporin, constipation, colon, fecal water content, rats

中图分类号:

R651.2

徐珮珮,杨明亮,刘长彬,李雅静,刘梓桐,李建军. 水通道蛋白过表达对脊髓损伤大鼠便秘的影响[J]. 《中国康复理论与实践》, 2020, 26(2): 181-188.

XU Pei-pei,YANG Ming-liang,LIU Chang-bin,LI Ya-jing,LIU Zi-tong,LI Jian-jun. Aquaporin Over-expression for Constipation in Rats with Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(2): 181-188.

图/表 11

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参考文献 44

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组别	n	术前	第1天	第3天	第7天	第14天	第28天	F值	P值
对照组	8	21.000±0.000	20.850±0.338	20.950±0.158	20.900±0.211	20.950±0.158	20.900±0.316	0.535	0.749
脊髓损伤组	8	21.000±0.000	0.000±0.000	0.100±0.211	4.100±0.738	7.100±0.460	9.700±0.350	4011.875	< 0.001
t值			195.373	250.200	69.230	90.135	75.132
P值			< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	n	术前	第3天	第14天	第28天	F值	P值
对照组	8	0.624±0.019	0.624±0.032	0.618±0.033	0.621±0.033	0.073	0.974
脊髓损伤组	8	0.621±0.018	0.523±0.023	0.538±0.016	0.547±0.015	44.840	< 0.001
t值		0.306	7.319	6.156	5.814
P值		0.764	< 0.001	< 0.001	< 0.001

组别	n	第3天	第14天	第28天	F值	P值
对照组	8	2.24±0.24	2.26±0.20	2.38±0.29	0.681	0.517
脊髓损伤组	8	5.44±0.77	5.85±0.88	5.53±0.53	0.688	0.514
t值		-11.263	-11.254	-14.823
P值		< 0.001	< 0.001	< 0.001

组别	n	第3天	第14天	第28天	F值	P值
对照组	8	2.39±0.16	2.31±0.18	2.37±0.14	0.518	0.603
脊髓损伤组	8	5.74±0.90	6.03±0.98	5.49±0.94	0.666	0.524
t值		-10.368	-10.569	-9.350
P值		< 0.001	< 0.001	< 0.001

组别	n	第3天	第14天	第28天	F值	P值
对照组	8	2.21±0.26	2.19±0.26	2.20±0.27	0.014	0.986
脊髓损伤组	8	5.14±0.16	5.40±0.13	5.25±0.92	0.085	0.919
t值		-5.165	-6.908	-9.041
P值		0.001	< 0.001	< 0.001

水通道蛋白过表达对脊髓损伤大鼠便秘的影响

Aquaporin Over-expression for Constipation in Rats with Spinal Cord Injury

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