电针对APP/PS1小鼠脑功能活动影响的功能性磁共振成像研究

doi:10.3969/j.issn.1006-9771.2020.05.010

《中国康复理论与实践》 ›› 2020, Vol. 26 ›› Issue (5): 544-549.doi: 10.3969/j.issn.1006-9771.2020.05.010

电针对APP/PS1小鼠脑功能活动影响的功能性磁共振成像研究

李乐^1,^2,³,黄盛^1,^2,³,李珑^1,^2,³,张嘉泳^1,^2,³,金婷婷^1,^2,³,张宇豪^1,^2,³,张冰雪^1,^2,³,杨敏光^1,^2,³,梁胜祥^1,^2,³,王志福^1,^2,³,柳维林^1,^2,³,陶静^1,^2,³,陈立典^1,^3,⁴()

1.福建中医药大学康复医学院,福建福州市 350122
2.福建省康复技术重点实验室,福建福州市 350122
3.福建康复产业研究院技术创新平台,福建福州市 350122
4.国家中医药管理局中医康复研究中心,福建福州市 350122

收稿日期:2019-08-10 修回日期:2019-09-18 出版日期:2020-05-25 发布日期:2020-05-29
通讯作者: 陈立典 E-mail:fjtcm1958@sina.com
作者简介:李乐(1994-),男,回族,安徽定远县人,硕士研究生,主要研究方向：神经康复学与认知科学。
基金资助:
国家自然科学基金项目(81774424)

Effects of Electroacupuncture on Brain Function of APP/PS1 Mice: A Functional Magnetic Resonance Imaging Study

LI Le^1,^2,³,HUANG Sheng^1,^2,³,LI Long^1,^2,³,ZHANG Jia-yong^1,^2,³,JIN Ting-ting^1,^2,³,ZHANG Yu-hao^1,^2,³,ZHANG Bing-xue^1,^2,³,YANG Min-guang^1,^2,³,LIANG Sheng-xiang^1,^2,³,WANG Zhi-fu^1,^2,³,LIU Wei-lin^1,^2,³,TAO Jing^1,^2,³,CHEN Li-dian^1,^3,⁴()

1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
2. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350122, China
3. Technology Innovation Platform of Fujian Rehabilitation Industry Research Institute, Fuzhou, Fujian 350122, China
4. Rehabilitation Research Center of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China

Received:2019-08-10 Revised:2019-09-18 Published:2020-05-25 Online:2020-05-29
Contact: CHEN Li-dian E-mail:fjtcm1958@sina.com
Supported by:
National Natural Science Foundation of China(81774424)

摘要/Abstract

摘要：

目的探讨电针百会(DU20)、神庭(DU24)对APP/PS1小鼠的脑功能活动的影响。方法同窝4月龄APP/PS1双转基因小鼠16只随机分为模型组(n = 8)和电针组(n = 8);同窝转基因阴性小鼠(n = 8)为对照组。电针组电针百会、神庭,共16周。干预前后进行新物体识别实验;小动物功能磁共振成像(fMRI)局部一致性(ReHo)分析法观察脑功能活动。结果干预后,与对照组相比,模型组新物体识别指数降低(P < 0.05);与模型组相比,电针组新物体识别指数增高( P < 0.05)。干预前,与对照组相比,模型组右侧基底前脑、左侧海马ReHo降低。干预后,与对照组相比,模型组双侧海马ReHo降低,压后皮质ReHo升高;与模型组相比,电针组双侧海马、运动皮质ReHo升高,前扣带回ReHo下降。结论电针百会、神庭可延缓APP/PS1小鼠学习记忆功能减退,可能与调节海马区的功能活动有关。

关键词: 阿尔茨海默病, 电针, 学习记忆, 脑功能, 功能磁共振成像, 小鼠

Abstract:

Objective To investigate the effects of electroacupuncture at Baihui (DU20) and Shenting (DU24) on brain function of APP/PS1 mice.Methods Sixteen 4-month-old APP/PS1 mice in the same litter were randomly divided into model group (n = 8) and electroacupuncture group (n = 8). Eight transgenic negative mice in the same litter were as control group. The electroacupuncture group accepted electroacupuncture at Baihui and Shenting for 16 weeks. They were assessed withObject Recognition Test before and after intervention, and observed under small animal functional magnetic resonance imaging with regional homogeneity (ReHo) analysis.Results Compared with the control group, the discrimination ratio decreased in the model group after intervention (P < 0.05), while it increased in the electroacupuncture group compared with that in the model group ( P < 0.05). Compared with the control group, ReHo of right basal forebrain and left hippocampus decreased in the model group before intervention. Compared with the control group, ReHo decreased in bilateral hippocampus group and increased in retrosplenial cortex in the model group after intervention; while it increased in bilateral hippocampus and motor cortex and decreased in anterior cingulate gyrus in the electroacupuncture group compared with that in the model group. Conclusion Electroacupuncture at Baihui and Shenting may delay the decline of learning and memory ability in Alzheimer's disease model mice, which may relate to the regulation of functional activities in hippocampus.

Key words: Alzheimer's disease, electroacupuncture, learning and memory, brain function, functional magnetic resonance imaging, mice

中图分类号:

R749.1

李乐,黄盛,李珑,张嘉泳,金婷婷,张宇豪,张冰雪,杨敏光,梁胜祥,王志福,柳维林,陶静,陈立典. 电针对APP/PS1小鼠脑功能活动影响的功能性磁共振成像研究[J]. 《中国康复理论与实践》, 2020, 26(5): 544-549.

LI Le,HUANG Sheng,LI Long,ZHANG Jia-yong,JIN Ting-ting,ZHANG Yu-hao,ZHANG Bing-xue,YANG Min-guang,LIANG Sheng-xiang,WANG Zhi-fu,LIU Wei-lin,TAO Jing,CHEN Li-dian. Effects of Electroacupuncture on Brain Function of APP/PS1 Mice: A Functional Magnetic Resonance Imaging Study[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(5): 544-549.

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参考文献 25

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组别	n	电针前	电针后
对照组	8	73.54±9.59	70.37±8.98^a
模型组	8	71.55±15.74	50.71±13.08
电针组	8	73.28±11.80	65.59±6.03^a
F值		0.058	6.191
P值		0.943	0.010

电针对APP/PS1小鼠脑功能活动影响的功能性磁共振成像研究

Effects of Electroacupuncture on Brain Function of APP/PS1 Mice: A Functional Magnetic Resonance Imaging Study

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