《中国康复理论与实践》

《中国康复理论与实践》 ›› 2020, Vol. 26 ›› Issue (5): 588-591.doi: 10.3969/j.issn.1006-9771.2020.05.019

• 临床研究 • 上一篇    下一篇

microRNA-137基因多态性与缺血性卒中后抑郁的相关性

符家武,吴昊(),李君良,叶烨,陈婧,周海红,李友   

  1. 广东医科大学附属医院神经内科,广东湛江市 524001
  • 收稿日期:2019-10-29 修回日期:2020-01-20 出版日期:2020-05-25 发布日期:2020-05-29
  • 通讯作者: 吴昊 E-mail:21803363@qq.com
  • 作者简介:符家武(1985-),男,汉族,广东湛江市人,硕士,主治医师,主要研究方向：脑血管病及精神障碍疾病基础与临床。
  • 基金资助:
    1. 国家自然科学基金项目(81571157);2. 湛江市科技计划项目(2019A01024)

Association between microRNA-137 Gene Polymorphisms and Ischemic Post-stroke Depression

FU Jia-wu,WU Hao(),LI Jun-liang,YE Ye,CHEN Jing,ZHOU Hai-hong,LI You   

  1. Department of Neurology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China
  • Received:2019-10-29 Revised:2020-01-20 Published:2020-05-25 Online:2020-05-29
  • Contact: WU Hao E-mail:21803363@qq.com
  • Supported by:
    National Science Foundation of China(81571157);Zhanjiang Research and Development Plan(2019A01024)

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摘要：

目的 分析microRNA-137基因多态性与缺血性卒中后抑郁的相关性。方法 2017年1月至2019年1月,SNaPshot检测缺血性卒中后抑郁患者250例(患者组)和健康体检者250例(对照组) microRNA-137基因rs1625579位点的基因型,逆转录荧光定量聚合酶链反应检测外周血单个核细胞microRNA-137相对表达量,并分析其与rs1625579位点不同基因型的关系。结果 患者组rs1625579位点等位基因T高于对照组(OR = 2.033, 95%CI 1.255~3.294, P= 0.003),基因型TT高于对照组 (OR = 2.139, 95%CI 1.272~3.595, P= 0.004);患者组microRNA-137相对表达量显著低于对照组(t = 28.230, P< 0.001);对照组TT基因型的microRNA-137相对表达量显著低于GT+GG基因型(t = 3.764, P< 0.001)。结论 microRNA-137基因rs1625579位点TT基因型可能通过下调microRNA-137的表达,参与缺血性卒中后抑郁的发病。

关键词: 缺血性脑卒中, 卒中后抑郁, microRNA-137, rs1625579, 基因多态性

Abstract:

Objective To analyze the relationship between microRNA-137 gene polymorphisms and ischemic post-stroke depression (PSD).Methods January, 2017 to January, 2019, the single nucleotide polymorphism (SNP) of rs1625579 in microRNA-137 was genotyped in 250 ischemic PSD patients and 250 healthy controls with SNaPshot. The expression of microRNA-137 was measured with quantitative real-time polymerase chain reaction in the mononuclear cells, and the association of the SNP rs1625579 with microRNA-137 expression was analyzed.Results The allele T was more in the patients than in the controls (OR = 2.033, 95%CI 1.255 to 3.294, P= 0.003), as well as the genotype TT (OR = 2.139, 95%CI 1.272~3.595, P= 0.004). The microRNA-137 expression was less in the patients than in the controls (t = 28.23, P< 0.001). For the controls, the microRNA-137 expression was also less in those with genotype of TT than with GT and GG (t = 3.764, P< 0.001).Conclusion The genotype TT of SNP rs1625579 in microRNA-137 is a risk factor of susceptibility to ischemic PSD, which may associate with the decrease of expression of microRNA-137.

Key words: ischemic stroke, post-stroke depression, microRNA-137, rs1625579, gene polymorphisms

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