脊髓损伤恒河猴下肢残留跨步能力的评估

doi:10.3969/j.issn.1006-9771.2020.06.005

《中国康复理论与实践》 ›› 2020, Vol. 26 ›› Issue (6): 648-653.doi: 10.3969/j.issn.1006-9771.2020.06.005

脊髓损伤恒河猴下肢残留跨步能力的评估

赵璨^1,²,魏瑞晗³,赵文⁴,季润⁵,饶家声^1,³(),杨朝阳⁴(),李晓光^3,⁴()

1.北京航空航天大学生物医学工程高精尖创新中心,北京市 100083
2.北京航空航天大学仪器科学与光电工程学院测控与信息技术系,北京市 100083
3.北京航空航天大学生物与医学工程学院生物材料与神经再生北京市重点实验室,北京市 100083
4.首都医科大学基础医学院神经生物学系,北京市 100069
5.国家康复辅具研究中心人体生物力学实验室,北京市 100176

收稿日期:2019-08-23 修回日期:2019-09-26 出版日期:2020-06-25 发布日期:2020-06-29
通讯作者: 饶家声,杨朝阳,李晓光 E-mail:raojschina@126.com;wack_lily@163.com;lxgchina@sina.com
作者简介:赵璨(1985-),女,汉族,湖南湘潭市人,博士,主要研究方向：脊髓损伤修复的行为学及影像学评估。
基金资助:
1.国家自然科学基金项目(31730030);国家自然科学基金项目(31900980);国家自然科学基金项目(31970970);2.国家重点研发计划项目(2017YFC1104002);国家重点研发计划项目(2017YFC1104001);3.中国博士后科学基金项目(2018M640046);4.北京市自然科学基金项目(7194286)

Evaluation of Residual Stepping Ability in Monkeys with Spinal Cord Injury

ZHAO Can^1,²,WEI Rui-han³,ZHAO Wen⁴,JI Run⁵,RAO Jia-sheng^1,³(),YANG Zhao-yang⁴(),LI Xiao-guang^3,⁴()

1. Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100083, China
2. Department of Measurement Control and Information Technology, School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100083, China
3. Beijing Key Laboratory for Biomaterials and Neural Regeneration, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China
4. Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069
5. Human Biomechanics Laboratory, National Research Center for Rehabilitation Technical Aids, Beijing 100176, China

Received:2019-08-23 Revised:2019-09-26 Published:2020-06-25 Online:2020-06-29
Contact: RAO Jia-sheng,YANG Zhao-yang,LI Xiao-guang E-mail:raojschina@126.com;wack_lily@163.com;lxgchina@sina.com
Supported by:
National Natural Science Foundation of China(31730030);National Natural Science Foundation of China(31900980);National Natural Science Foundation of China(31970970);National Key Research and Development Program(2017YFC1104002);National Key Research and Development Program(2017YFC1104001);China Postdoctoral Science Foundation(2018M640046);Natural Science Foundation of Beijing(7194286)

摘要/Abstract

摘要：

目的观察评估脊髓损伤后恒河猴的下肢残留跨步能力。方法成年雌性恒河猴4只,胸椎T_7-9右半侧切除脊髓组织1 cm。分别在脊髓损伤前、脊髓损伤后6周和12周,采用VICON系统进行双下肢步态测试,获取动物在跑步机上连续跨步中的双下肢步态周期时长,步长、步高、膝/踝关节角度幅值以及联动参数比值,并量化分析。结果脊髓损伤后,恒河猴双下肢的协调性破坏,右下肢明显拖拽;左下肢步态周期时长显著增加(P < 0.001),膝/踝关节角度屈曲/伸展的幅值均显著增大( P < 0.001)。联动参数比值在脊髓损伤前后均无显著性差异( P > 0.05)。左下肢步态周期时长与步长( r= 0.838, P = 0.001)、步高(r= 0.726, P = 0.007)和踝关节角度变化幅值(r= 0.766, P = 0.004)均相关,踝关节角度变化幅值与步长呈正相关(r= 0.627, P = 0.029)。结论脊髓损伤后恒河猴健侧肢体步态模式发生改变。健侧下肢代偿性调整运动策略,以适应患侧下肢功能的缺失。

关键词: 恒河猴, 脊髓半侧损伤, 步态分析, 残留跨步能力

Abstract:

Objective To evaluate the residual stepping ability in monkeys with spinal cord injury longitudinally.Methods Four adult female monkeys were studied. Right hemisection of 10 mm spinal cord tissue was performed at the T_7-9 segment. Gait tests of bipedal locomotion were performed before, and six weeks and twelve weeks after injury by VICON system. Gait cycle duration, amplitude of knee and ankle angles, and ratio of united parameters were obtained from successive stepping and were quantitative analyzed.Results The coordination of bilateral hindlimbs was destroyed after spinal cord injury, and the right hindlimb showed obviously dragging. The gait cycle duration of the left hindlimb increased significantly (P < 0.001), and the amplitudes of knee and ankle angle significantly increased ( P < 0.001) after spinal cord injury. The ratio of united parameters was not statistically different among all the time points ( P> 0.05). The gait cycle duration of the left hindlimb was correlated with step length (r= 0.838, P = 0.001), step height (r= 0.726, P = 0.007) and amplitude of ankle angle (r= 0.766, P = 0.004), and the amplitude of ankle angle was correlated with step length (r= 0.627, P = 0.029).Conclusion The gait pattern of monkey with spinal cord injury has been changed. The gait strategy of the uninjured side was adjusted compensatively after spinal cord injury to adapt the functional impairment of contralateral hindlimb.

Key words: Rhesus monkeys, spinal cord hemisection, gait analysis, residual stepping ability

中图分类号:

R651.2

赵璨,魏瑞晗,赵文,季润,饶家声,杨朝阳,李晓光. 脊髓损伤恒河猴下肢残留跨步能力的评估[J]. 《中国康复理论与实践》, 2020, 26(6): 648-653.

ZHAO Can,WEI Rui-han,ZHAO Wen,JI Run,RAO Jia-sheng,YANG Zhao-yang,LI Xiao-guang. Evaluation of Residual Stepping Ability in Monkeys with Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(6): 648-653.

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时间	n	步态周期
脊髓损伤前	4	1.51±0.07
脊髓损伤6周	4	2.11±0.09^a
脊髓损伤12周	4	2.06±0.09^a,b
F值		16.510
P值		< 0.001

时间	n	踝关节	膝关节
脊髓损伤前	4	33.06±1.86	37.35±1.44
脊髓损伤6周	4	46.70±2.09^a	46.70±2.09^a
脊髓损伤12周	4	46.28±2.71^a,b	52.27±1.80^a,b
F值		11.870	19.070
P值		< 0.001	< 0.001

时间	n	步长/步高	膝关节角度变化幅值/踝关节角度变化幅值
脊髓损伤前	4	9.78±1.16	1.00±0.07
脊髓损伤6周	4	12.09±1.99^a	0.92±0.10^d
脊髓损伤12周	4	8.31±1.35^b,c	0.97±0.05^e,f
F值		0.946	0.997
P值		> 0.05	> 0.05

脊髓损伤恒河猴下肢残留跨步能力的评估

Evaluation of Residual Stepping Ability in Monkeys with Spinal Cord Injury

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