骨髓间充质干细胞衍生外泌体对脊髓损伤动物治疗作用的系统综述

doi:10.3969/j.issn.1006-9771.2022.05.014

《中国康复理论与实践》 ›› 2022, Vol. 28 ›› Issue (5): 585-592.doi: 10.3969/j.issn.1006-9771.2022.05.014

骨髓间充质干细胞衍生外泌体对脊髓损伤动物治疗作用的系统综述

魏娟芳¹,王琳杰²,崔艳如¹,岑秋宇¹,张安仁³()

1.成都中医药大学养生康复学院,四川成都市 610075
2.西南交通大学医学院,四川成都市 610031
3.同济大学附属上海市第四人民医院康复医学科,上海市 200434

收稿日期:2021-11-30 修回日期:2022-03-29 出版日期:2022-05-25 发布日期:2022-06-10
通讯作者: 张安仁 E-mail:1518526780@qq.com
作者简介:魏娟芳(1997-),女,汉族,甘肃天水市人,硕士研究生,主要研究方向：脊髓损伤康复的临床及基础研究
基金资助:
国家自然科学基金项目(81973927)

Effects of bone marrow mesenchymal stem cells derived exosomes on spinal cord injured animals: a systematic review

WEI Juanfang¹,WANG Linjie²,CUI Yanru¹,CEN Qiuyu¹,ZHANG Anren³()

1. College of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, China
2. School of Medicine, Southwest Jiaotong University, Chengdu, Sichuan 610031, China
3. Department of Rehabilitation Medicine, Shanghai Fourth People's Hospital Affiliated to Tongji University, Shanghai 200434, China

Received:2021-11-30 Revised:2022-03-29 Published:2022-05-25 Online:2022-06-10
Contact: ZHANG Anren E-mail:1518526780@qq.com
Supported by:
National Natural Science Foundation of China(81973927)

摘要/Abstract

摘要：

目的系统评价骨髓间充质干细胞(BMSC)来源的外泌体治疗脊髓损伤动物的有效性和安全性。方法从PubMed、Cochrane Library、Web of Science、CNKI、万方数据库中检索自建库至2021年10月BMSC来源的外泌体治疗脊髓损伤的动物研究。由2名研究者独立筛选、提取资料并评价偏倚风险后,采用定性分析方法评估结果。结果共纳入21项研究,包括1 342个动物。18项研究选用雄性或雌性Sprague-Dawley大鼠,19项研究大鼠体质量为(200±50) g,损伤部位集中于T_9-11脊髓,但具体方式不同。外泌体的类型、使用时间、频率、浓度和剂量等均不相同。结论 BMSC来源的外泌体可改善脊髓损伤后运动功能,减少受损组织,抗凋亡,抗炎,减轻血-脊髓屏障通透性,促进轴突和血管生长。仍有待更多高质量研究验证。

关键词: 脊髓损伤, 外泌体, 骨髓间充质干细胞, 动物实验, 系统综述

Abstract:

Objective To systematically review the efficacy and safety of exosomes derived from bone marrow mesenchymal stem cells (BMSC) on animal models of spinal cord injury. Methods Animal studies about BMSC-derived exosomes for spinal cord injury were retrieved from databases of PubMed, Cochrane Library, Web of Science, CNKI and Wangfang Data, from establishment to October, 2021. Two researchers independently screened and extracted the data, and evaluated the risk of bias. The studies were qualitatively analyzed. Results A total of 21 studies were included, involving 1 342 animals. Male or female Sprague-Dawley rats were selected for 18 studies, and the body mass of the rats was (200±50) g in 19 studies. The injury nodes focused on T_9-11 spinal cord, with various methods. The types, medication time, frequency, concentration and dose of the exosomes were heterogeneous. Conclusions The BMSC-derived exosomes can improve the motor function after spinal cord injury, reduce the damage of spinal cord, resist apoptosis and inflammation, reduce the permeability of blood-spinal cord barrier, and promote the growth of axons and blood vessels. More high-quality studies are needed for further verification.

Key words: spinal cord injury, exosomes, bone marrow mesenchymal stem cells, animal experiment, systematic review

中图分类号:

R651.2

魏娟芳,王琳杰,崔艳如,岑秋宇,张安仁. 骨髓间充质干细胞衍生外泌体对脊髓损伤动物治疗作用的系统综述[J]. 《中国康复理论与实践》, 2022, 28(5): 585-592.

WEI Juanfang,WANG Linjie,CUI Yanru,CEN Qiuyu,ZHANG Anren. Effects of bone marrow mesenchymal stem cells derived exosomes on spinal cord injured animals: a systematic review[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 585-592.

图/表 6

表1

表2

图1

表3

纳入研究的一般情况"

纳入研究	动物种属	体质量/ 年龄	n	节段	造模方式	干预措施		结局指标
纳入研究	动物种属	体质量/ 年龄	n	节段	造模方式	T	C	结局指标
Chang等^[10](2021)	雄SD大鼠	220~260 g	72	T₁₀	挫伤：10 g杆12.5 mm高释放	Ⅰ	PBS	①②③④
Chen等^[11](2021)	雄SD大鼠	6~8周	18	T₁₀	钳夹：75 g夹闭30 s	Ⅱ	PBS	①②④⑤⑥
Gu等^[14](2020)	雄SD大鼠	220~260 g	30	T₁₀	挫伤：10 g杆12.5 mm高释放	Ⅱ	PBS	①③
Huang等^[21](2017)	雄SD大鼠	180~220 g	30	T₁₀	挫伤：8 g棒40 mm高释放	Ⅱ	PBS	①③④⑦
Huang等^[19](2021)	雌SD大鼠	200~250 g 10周	100	T₁₀	挫伤：30 g重物50 mm高释放	Ⅱ	PBS	①③④⑤⑧⑨
Jia等^[28](2021)a	雄SD大鼠	230~250 g	40	T₁₀	挫伤：2 N	Ⅱ	生理盐水	①②③
Jia等^[29](2021)b	雄SD大鼠	230~250 g	50	T₁₀	挫伤：2 N	Ⅱ	生理盐水	①②③
Li等^[30](2019)	雄Wistar大鼠	150~200 g	150	T_9-11	挫伤：10 g杆5 cm高释放	Ⅱ	PBS	①②③
Liu等^[31](2022)	雄SD大鼠	250~300 g/ 6~8周	30	T_9-11	挫伤：10 g杆6.5 cm高释放	Ⅱ	PBS	①②④
Liu等^[13](2020)	雄C57BL/6小鼠	6~8周	56	T₁₀	挫伤：5 g杆6.5 cm高释放	Ⅱ	PBS	①②④⑤
Liu等^[32](2019)	雌SD大鼠	170~220 g	20	T₁₀	挫伤：直径2.5 mm打击器12.5 mm高释放	Ⅱ	PBS	①②④
Lu等^[22](2019)	雄SD大鼠	200~250 g	100	T₁₀	挫伤	Ⅱ	PBS	①②③⑩
Luo等^[15](2021)	雌SD大鼠	170~220 g	40	T₁₀	挫伤：10 g杆12.5 mm高释放	Ⅱ	PBS	①②③
Yu等^[33](2019)	雌SD大鼠	230~250 g	80	T₁₀	挫伤：2 N	Ⅱ	PBS	①②
Zhang等^[34](2021)	雄SD大鼠	200~230 g	48	T₉	挫伤：10 g杆12.5 mm高释放	Ⅱ	PBS	①②④
Zhao等^[35](2019)	雄Wistar大鼠	200~250 g	73	T₁₀	横断:虹膜刀水平切断右侧半圆形脊髓	Ⅱ	PBS	①②④?
Zhou等^[5](2022)	雄SD大鼠	200~250 g	160	T₁₀	挫伤：2 N	Ⅱ	PBS	①②③④⑩
董万亮^[36](2020)	雄SD大鼠	约200 g	75	T₁₀	挫伤：20 g棒4.5 cm高释放	Ⅱ	磷酸盐溶液	①?
裴双等^[37](2017)	雄SD大鼠	180~200 g	30	T₁₀	挫伤：2 N	Ⅱ	磷酸盐溶液	①②?
张锐毅^[17](2019)	雄SD大鼠	200~250 g	60	T₁₀	挫伤：2 N	Ⅱ	PBS	①
周燕等^[38](2019)	雄SD大鼠	220~250 g	80	T₁₀	挫伤：20 kPa	Ⅱ	PBS	①③④

表3

表4

表5

参考文献 43

[1]	ANJUM A, YAZID M D, FAUZI DAUD M, et al. Spinal cord injury: pathophysiology, multimolecular interactions, and underlying recovery mechanisms[J]. Int J Mol Sci, 2020, 21(20): 7533. doi: 10.3390/ijms21207533
[2]	BARNABÉ-HEIDER F, GÖRITZ C, SABELSTRÖM H, et al. Origin of new glial cells in intact and injured adult spinal cord[J]. Cell Stem Cell, 2010, 7(4): 470-482. doi: 10.1016/j.stem.2010.07.014
[3]	BRADBURY E J, BURNSIDE E R. Moving beyond the glial scar for spinal cord repair[J]. Nat Commun, 2019, 10(1): 3879. doi: 10.1038/s41467-019-11707-7
[4]	DIAS D O, GÖRITZ C. Fibrotic scarring following lesions to the central nervous system[J]. Matrix Biol, 2018, 68-69: 561-570. doi: 10.1016/j.matbio.2018.02.009
[5]	ZHOU Y, WEN L L, LI Y F, et al. Exosomes derived from bone marrow mesenchymal stem cells protect the injured spinal cord by inhibiting pericyte pyroptosis[J]. Neural Regen Res, 2022, 17(1): 194-202. doi: 10.4103/1673-5374.314323
[6]	XU H, LEE C W, WANG Y F, et al. The role of paracrine regulation of mesenchymal stem cells in the crosstalk with macrophages in musculoskeletal diseases: a systematic review[J]. Front Bioeng Biotechnol, 2020, 8: 587052. doi: 10.3389/fbioe.2020.587052
[7]	YUAN X, WU Q, WANG P, et al. Exosomes derived from pericytes improve microcirculation and protect blood-spinal cord barrier after spinal cord injury in mice[J]. Front Neurosci, 2019, 13: 319. doi: 10.3389/fnins.2019.00319
[8]	REN Z, QI Y, SUN S, et al. Mesenchymal stem cell-derived exosomes: hope for spinal cord injury repair[J]. Stem Cells Dev, 2020, 29(23): 1467-1478. doi: 10.1089/scd.2020.0133
[9]	LI C, QIN T, ZHAO J, et al. Bone marrow mesenchymal stem cell-derived exosome-educated macrophages promote functional healing after spinal cord injury[J]. Front Cell Neurosci, 2021, 15: 725573. doi: 10.3389/fncel.2021.725573
[10]	CHANG Q, HAO Y, WANG Y, et al. Bone marrow mesenchymal stem cell-derived exosomal microRNA-125a promotes M2 macrophage polarization in spinal cord injury by downregulating IRF5[J]. Brain Res Bull, 2021, 170: 199-210. doi: 10.1016/j.brainresbull.2021.02.015
[11]	CHEN Y, TIAN Z, HE L, et al. Exosomes derived from miR-26a-modified MSCs promote axonal regeneration via the PTEN/AKT/mTOR pathway following spinal cord injury[J]. Stem Cell Res Ther, 2021, 12(1): 224. doi: 10.1186/s13287-021-02282-0
[12]	FAN L, DONG J, HE X, et al. Bone marrow mesenchymal stem cells-derived exosomes reduce apoptosis and inflammatory response during spinal cord injury by inhibiting the TLR4/MyD88/NF-κB signaling pathway[J]. Hum Exp Toxicol, 2021, 40(10): 1612-1623. doi: 10.1177/09603271211003311
[13]	LIU W, RONG Y, WANG J, et al. Exosome-shuttled miR-216a-5p from hypoxic preconditioned mesenchymal stem cells repair traumatic spinal cord injury by shifting microglial M1/M2 polarization[J]. J Neuroinflammation, 2020, 17(1): 47. doi: 10.1186/s12974-020-1726-7
[14]	GU J, JIN Z S, WANG C M, et al. Bone marrow mesenchymal stem cell-derived exosomes improves spinal cord function after injury in rats by activating autophagy[J]. Drug Des Devel Ther, 2020, 14: 1621-1631. doi: 10.2147/DDDT.S237502
[15]	LUO Y, XU T, LIU W, et al. Exosomes derived from GIT1-overexpressing bone marrow mesenchymal stem cells promote traumatic spinal cord injury recovery in a rat model[J]. Int J Neurosci, 2021, 131(2): 170-182. doi: 10.1080/00207454.2020.1734598
[16]	LIU W, MA Z, LI J, et al. Mesenchymal stem cell-derived exosomes: therapeutic opportunities and challenges for spinal cord injury[J]. Stem Cell Res Ther, 2021, 12(1): 102. doi: 10.1186/s13287-021-02153-8
[17]	张锐毅. 骨髓间充质干细胞来源外泌体对大鼠脊髓损伤后周细胞活化及血脊髓屏障的影响[D]. 郑州: 郑州大学, 2019.
	ZHANG R Y. Effects of exosomes derived from bone marrow mesenchymal stem cells on peripheral cell activation and blood spinal cord barrier after spinal cord injury in rats[D]. Zhengzhou: Zhengzhou University, 2019.
[18]	HUANG J H, XU Y, YIN X M, et al. Exosomes derived from miR-126-modified MSCs promote angiogenesis and neurogenesis and attenuate apoptosis after spinal cord injury in rats[J]. Neuroscience, 2020, 424: 133-145. doi: 10.1016/j.neuroscience.2019.10.043
[19]	HUANG W, QU M, LI L, et al. SiRNA in MSC-derived exosomes silences CTGF gene for locomotor recovery in spinal cord injury rats[J]. Stem Cell Res Ther, 2021, 12(1): 334. doi: 10.1186/s13287-021-02401-x
[20]	WANG L, PEI S, HAN L, et al. Mesenchymal stem cell-derived exosomes reduce A1 astrocytes via downregulation of phosphorylated NFκB P65 subunit in spinal cord injury[J]. Cell Physiol Biochem, 2018, 50(4): 1535-1559. doi: 10.1159/000494652
[21]	HUANG J H, YIN X M, XU Y, et al. Systemic administration of exosomes released from mesenchymal stromal cells attenuates apoptosis, inflammation, and promotes angiogenesis after spinal cord injury in rats[J]. J Neurotrauma, 2017, 34(24): 3388-3396. doi: 10.1089/neu.2017.5063
[22]	LU Y, ZHOU Y, ZHANG R, et al. Bone mesenchymal stem cell-derived extracellular vesicles promote recovery following spinal cord injury via improvement of the integrity of the blood-spinal cord barrier[J]. Front Neurosci, 2019, 13: 209. doi: 10.3389/fnins.2019.00209
[23]	XIN W, QIANG S, JIANING D, et al. Human bone marrow mesenchymal stem cell-derived exosomes attenuate blood-spinal cord barrier disruption via the TIMP2/MMP pathway after acute spinal cord injury[J]. Mol Neurobiol, 2021, 58(12): 6490-6504. doi: 10.1007/s12035-021-02565-w
[24]	HOOIJMANS C R, ROVERS M M, DE VRIES R B, et al. SYRCLE's risk of bias tool for animal studies[J]. BMC Med Res Methodol, 2014, 14: 43. doi: 10.1186/1471-2288-14-43
[25]	LEWIN S, BOOTH A, GLENTON C, et al. Applying GRADE-CERQual to qualitative evidence synthesis findings: introduction to the series[J]. Implement Sci, 2018, 13(Suppl 1): 2. doi: 10.1186/s13012-017-0688-3
[26]	陈莉琳, 黄牡丹, 郑海清. 脑卒中后肢体痉挛的识别与评估:Scoping综述[J]. 中国康复理论与实践, 2022, 28(1): 62-68.
	CHEN L L, HUANG M D, ZHENG H Q. Identification and evaluation of post-stroke spasticity: a scoping review[J]. Chin J Rehabil Theory Pract, 2022, 28(1): 62-68.
[27]	王丽群, 庞日朝, 刘建成, 等. 靶向线粒体治疗脊髓损伤的疗效:基于动物实验的系统评价[J]. 中国康复理论与实践, 2021, 27(5): 574-582.
	WANG L Q, PANG R Z, LIU J C, et al. Effect of targeting mitochondria on spinal cord injury: a systematic review based on animal experiments[J]. Chin J Rehabil Theory Pract, 2021, 27(5): 574-582.
[28]	JIA Y, LU T, CHEN Q, et al. Exosomes secreted from sonic hedgehog-modified bone mesenchymal stem cells facilitate the repair of rat spinal cord injuries[J]. Acta Neurochir (Wien), 2021, 163(8): 2297-2306. doi: 10.1007/s00701-021-04829-9
[29]	JIA Y, YANG J, LU T, et al. Repair of spinal cord injury in rats via exosomes from bone mesenchymal stem cells requires sonic hedgehog[J]. Regen Ther, 2021, 18: 309-315.
[30]	LI C, JIAO G, WU W, et al. Exosomes from bone marrow mesenchymal stem cells inhibit neuronal apoptosis and promote motor function recovery via the Wnt/β-catenin signaling pathway[J]. Cell Transplant, 2019, 28(11): 1373-1383. doi: 10.1177/0963689719870999
[31]	LIU J, LIN M, QIAO F, et al. Exosomes derived from lncRNA TCTN2-modified mesenchymal stem cells improve spinal cord injury by miR-329-3 p/IGF1R axis[J]. J Mol Neurosci, 2022, 72(3): 482-495. doi: 10.1007/s12031-021-01914-7. Epub 2021-10-08. doi: 10.1007/s12031-021-01914-7. Epub
[32]	LIU W, WANG Y, GONG F, et al. Exosomes derived from bone mesenchymal stem cells repair traumatic spinal cord injury by suppressing the activation of A1 neurotoxic reactive astrocytes[J]. J Neurotrauma, 2019, 36(3): 469-484. doi: 10.1089/neu.2018.5835
[33]	YU T, ZHAO C, HOU S, et al. Exosomes secreted from miRNA-29b-modified mesenchymal stem cells repaired spinal cord injury in rats[J]. Braz J Med Biol Res, 2019, 52(12): e8735. doi: 10.1590/1414-431x20198735
[34]	ZHANG M, WANG L, HUANG S, et al. Exosomes with high level of miR-181c from bone marrow-derived mesenchymal stem cells inhibit inflammation and apoptosis to alleviate spinal cord injury[J]. J Mol Histol, 2021, 52(2): 301-311. doi: 10.1007/s10735-020-09950-0
[35]	ZHAO C, ZHOU X, QIU J, et al. Exosomes derived from bone marrow mesenchymal stem cells inhibit complement activation in rats with spinal cord injury[J]. Drug Des Devel Ther, 2019, 13: 3693-3704. doi: 10.2147/DDDT.S209636
[36]	董万亮. 骨髓间充质干细胞来源的外泌体通过TSG-6通路促进脊髓损伤修复的作用及机制研究[D]. 郑州: 郑州大学, 2020.
	DONG W L. The role and mechanism of exosomes derived from bone marrow mesenchymal stem cells in promoting the repair of spinal cord injury through TSG-6 pathway[D]. Zhengzhou: Zhengzhou University, 2020.
[37]	裴双, 王琳, 陈雪梅, 等. 骨髓间充质干细胞来源的外泌体静脉移植对脊髓损伤的修复作用[J]. 中国脊柱脊髓杂志, 2017, 27(12): 1119-1127.
	PEI S, WANG L, CHEN X M, et al. Repair of spinal cord injury by intravenous transplantation of exosomes derived from bone marrow mesenchymal stem cells[J]. Chin J Spine Spinal Cord, 2017, 27(12): 1119-1127.
[38]	周燕, 王琳, 裴双, 等. 骨髓间充质干细胞外泌体可减少脊髓损伤后A1型星形胶质细胞的活化[J]. 中国组织工程研究, 2019, 23(21): 3294-3301.
	ZHOU Y, WANG L, PEI S, et al. Bone marrow mesenchymal stem cell-derived exosomes reduce the activation of type A1 astrocytes after spinal cord injury[J]. ChinJ Tiss Eng Res, 2019, 23(21): 3294-3301.
[39]	H RASHED M, BAYRAKTAR E, K HELAL G, et al. Exosomes: from garbage bins to promising therapeutic targets[J]. Int J Mol Sci, 2017, 18(3): 538. doi: 10.3390/ijms18030538
[40]	YANG C, GUO W B, ZHANG W S, et al. Comprehensive proteomics analysis of exosomes derived from human seminal plasma[J]. Andrology, 2017, 5(5): 1007-1015. doi: 10.1111/andr.12412
[41]	ZHANG D, LEE H, ZHU Z, et al. Enrichment of selective miRNAs in exosomes and delivery of exosomal miRNAs in vitro and in vivo[J]. Am J Physiol Lung Cell Mol Physiol, 2017, 312(1): L110-L121.
[42]	DOMENIS R, CIFÙ A, QUAGLIA S, et al. Pro inflammatory stimuli enhance the immunosuppressive functions of adipose mesenchymal stem cells-derived exosomes[J]. Sci Rep, 2018, 8(1): 13325. doi: 10.1038/s41598-018-31707-9
[43]	HU G W, LI Q, NIU X, et al. Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells attenuate limb ischemia by promoting angiogenesis in mice[J]. Stem Cell Res Ther, 2015, 6(1): 10. doi: 10.1186/scrt546

生物效应	作用机制
促进轴突生长	减少硫酸软骨素蛋白聚糖产生,增加生长相关蛋白和神经丝表达,缓解神经传导障碍^[8-9]
促进血管生成	上调参与人脐静脉内皮细胞血管生成的关键基因表达,促进血管内皮生长因子、血管生成素等的表达^[8]
抗炎	促进巨噬细胞M2表型极化,降低炎症因子表达^[10];抑制A1星形胶质细胞活化^[11];抑制脂多糖诱导的NF-κB通路激活^[12];促进小胶质细胞M2表型极化^[13]
抗凋亡	下调caspase-3/9等表达,增加Bcl-2表达,增强Wnt/β-catenin表达^[8,12];促进早期自噬^[14];减少谷氨酸表达^[15]
降低BSCB通透性	通过NF-κB p65通路抑制周细胞迁移^[16];减少claudin-5、ZO-1等的丢失^[17];抑制周细胞焦亡^[5]

结局指标	评定方法
运动功能	①Basso-Beattie-Bresnahan (BBB)评分。②水平爬梯测试^[18]。③运动诱发电位。④尿斑面积定量。⑤热敏度：爪子缩回热潜伏期^[19]。⑥斜板实验^[20]。⑦步态分析^[17]
组织病理学	①HE染色。②免疫荧光Hoechst染色^[11]。③尼氏染色。④透射电子显微镜^[20];Fluoro-Jade B染色^[5]
凋亡	①TUNEL染色^[10]。②凋亡相关标志物^[8]
炎症	①炎性因子和抗炎因子表达^[10]。②炎症相关标志物表达^[11]。③巨噬细胞、小胶质细胞、星形胶质细胞表型极化。④NF-κB信号通路
轴突生长	①免疫荧光染色。②相关基因和蛋白水平^[11]
血管生长	①血管生成相关因子表达^[18]。②内皮细胞免疫荧光染色^[21]
BSCB通透性	①伊文思蓝评估^[22]。②FITC-葡聚糖分析^[23]。③紧密连接蛋白^[17]

结局指标	n	方法学局限性	相关性	结果一致性	数据充分性	总体评价
运动功能	21	21项研究均存在轻微实施偏倚,9项存在轻微评估偏倚,17项存在轻微报告偏倚,1项存在中度报告偏倚	纳入标准与研究问题基本一致	均报告运动功能得到改善	均进行定量分析,资料充分	高
组织病理学	19	19项研究均存在轻微实施偏倚,8项存在轻微评估偏倚,16项存在轻微报告偏倚	纳入标准与研究问题基本一致	均报告脊髓组织病理得到改善	均进行定量或定性分析,资料充分	高
凋亡	11	11项研究均存在轻微实施偏倚,3项存在轻微评估偏倚,10项存在轻微报告偏倚	纳入标准与研究问题基本一致	均报告细胞凋亡得到改善	均进行定量或定性分析,资料相对充分	中
炎症	11	11项研究均存在轻微实施偏倚,5项存在轻微评估偏倚,10项存在轻微报告偏倚	纳入标准与研究问题基本一致	均报告炎症得到改善	均进行定量或定性分析,资料相对充分	中
BSCB通透性	3	3项研究均存在轻微实施偏倚,1项存在轻微评估偏倚,2项存在轻微报告偏倚	纳入标准与研究问题一致	均报告BSCB渗透性得到改善	定性分析,资料不充分	低
轴突生长	1	存在轻微的实施、评估和报告偏倚	纳入标准与研究问题一致	报告轴突生长得到改善	定性分析,资料不充分	低
血管生长	1	存在轻微的实施和报告偏倚	纳入标准与研究问题一致	报告血管生长得到改善	定性分析,资料不充分	低

骨髓间充质干细胞衍生外泌体对脊髓损伤动物治疗作用的系统综述

Effects of bone marrow mesenchymal stem cells derived exosomes on spinal cord injured animals: a systematic review

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 43

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	王航宇, 葛可可, 范永红, 都丽露, 邹敏, 封磊. 基于ICD-11和ICF主动式音乐疗法改善认知障碍老年人认知功能的系统综述[J]. 《中国康复理论与实践》, 2024, 30(1): 36-43.
[2]	闻嘉宁, 金秋艳, 张琦, 李杰, 司琦. 认知参与型身体活动对发展儿童青少年执行功能的效果：基于ICF的系统综述[J]. 《中国康复理论与实践》, 2024, 30(1): 44-53.
[3]	葛可可, 范永红, 王航宇, 都丽露, 李长江, 邹敏. 失眠老年人正念干预健康效益的系统综述[J]. 《中国康复理论与实践》, 2024, 30(1): 54-60.
[4]	刘冬, 徐子涵, 李江, 鞠萍. M1区联合背外侧前额叶高频重复经颅磁刺激对脊髓损伤后神经病理性疼痛患者脑电图θ振幅的效果[J]. 《中国康复理论与实践》, 2024, 30(1): 87-94.
[5]	张婧雅, 邹敏, 孙宏伟, 孙昌隆, 朱峻同. 听障儿童青少年焦虑或抑郁情绪心理干预效果的系统综述[J]. 《中国康复理论与实践》, 2023, 29(9): 1004-1011.
[6]	王俊宇, 杨永, 袁逊, 谢婷, 庄洁. 高强度间歇训练对健康儿童青少年执行功能效果的系统综述[J]. 《中国康复理论与实践》, 2023, 29(9): 1012-1020.
[7]	魏晓微, 杨剑, 魏春艳. 特殊教育学校孤独症谱系障碍儿童参与适应性瑜伽活动的心理与行为效益的系统综述[J]. 《中国康复理论与实践》, 2023, 29(9): 1021-1028.
[8]	杨亚茹, 杨剑. 基于WHO-HPS架构学校身体活动相关健康服务及其健康效益：系统综述的系统综述[J]. 《中国康复理论与实践》, 2023, 29(9): 1040-1047.
[9]	史佳伟, 李凌宇, 杨浩杰, 王琴潞, 邹海欧. 预康复对全膝关节置换术后患者的有效性：系统综述的系统综述[J]. 《中国康复理论与实践》, 2023, 29(9): 1057-1064.
[10]	蒋长好, 黄辰, 高晓妍, 戴元富, 赵国明. 神经反馈训练对老年人认知功能效果的系统综述[J]. 《中国康复理论与实践》, 2023, 29(8): 903-909.
[11]	魏晓微, 杨剑, 魏春艳, 贺启令. 学校环境下适应性体育课程促进智力与发展性残疾儿童心理运动发展的系统综述[J]. 《中国康复理论与实践》, 2023, 29(8): 910-918.
[12]	李芳, 霍速, 杜巨豹, 刘秀贞, 李小爽, 宋为群. 经颅直流电刺激联合任务导向性康复训练对脊髓损伤大鼠前肢运动障碍的效果[J]. 《中国康复理论与实践》, 2023, 29(7): 777-781.
[13]	王少璞, 陈钢. 基于世界卫生组织健康促进学校架构的心理行为健康服务及其健康效益：系统综述的系统综述[J]. 《中国康复理论与实践》, 2023, 29(7): 800-807.
[14]	刘宁, 刘雨泉, 祝斌, 于凌佳, 谭海宁, 杨雍, 李想. 脊髓损伤神经学分类国际标准国内应用情况的文献计量学研究[J]. 《中国康复理论与实践》, 2023, 29(7): 808-815.
[15]	王一吉, 周红俊, 何泽佳, 刘根林, 郑樱, 郝春霞, 卫波, 康海琼, 张缨, 逯晓蕾, 袁媛, 蒙倩茹. 不完全性脊髓损伤患者运动功能对称性与步态对称性的关系[J]. 《中国康复理论与实践》, 2023, 29(6): 639-645.