《中国康复理论与实践》 ›› 2022, Vol. 28 ›› Issue (8): 927-933.doi: 10.3969/j.issn.1006-9771.2022.08.008
关莹1,2,于国强1,2,唐祎周1,2,辛贵乐1,2,李季1,2,王璐1,2,张立1,2,张春艳1,2()
收稿日期:
2022-02-24
修回日期:
2022-05-11
出版日期:
2022-08-25
发布日期:
2022-08-30
通讯作者:
张春艳
E-mail:676477397@qq.com
作者简介:
关莹(1982-),女,满族,辽宁沈阳市人,博士,副主任医师,主要研究方向:脑卒中后功能障碍的中医康复研究。
基金资助:
GUAN Ying1,2,YU Guoqiang1,2,TANG Yizhou1,2,XIN Guile1,2,LI Ji1,2,WANG Lu1,2,ZHANG Li1,2,ZHANG Chunyan1,2()
Received:
2022-02-24
Revised:
2022-05-11
Published:
2022-08-25
Online:
2022-08-30
Contact:
ZHANG Chunyan
E-mail:676477397@qq.com
Supported by:
摘要:
目的 观察头穴艾灸联合跑台训练对血管性痴呆(VaD)模型大鼠学习记忆能力和氧化应激损伤的影响。
方法 健康雄性Sprague-Dawley大鼠40只,随机分为假手术组、模型组、针康组和灸康组,每组10只。后三组采用双侧颈动脉结扎法制备VaD模型大鼠。造模后6周采用Morris水迷宫测试从造模大鼠中筛选出明显认知障碍大鼠每组8只,假手术组随机挑选8只。针康组予头穴丛刺结合跑台训练,灸康组予头穴艾灸结合跑台训练,共4周。干预后行Morris水迷宫测试,检测海马组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和谷胱甘肽过氧化物还原酶(GSH-Px)水平。
结果 与假手术组相比,模型组大鼠逃避潜伏期显著延长,穿越平台次数显著减少,目标象限停留时间显著缩短(P < 0.001),SOD、GSH、GSH-Px水平显著下降,MDA水平显著增加(P < 0.001);针康组和灸康组实验第3天起逃避潜伏期较模型组显著缩短(P < 0.001),穿越平台次数显著增多,目标象限停留时间显著延长(P < 0.001);SOD、GSH、GSH-Px水平显著增高,MDA水平显著下降(P < 0.001),且灸康组显著优于针康组(P < 0.001)。
结论 与针康法相比,头穴艾灸结合跑台训练可进一步降低VaD大鼠海马组织氧化应激水平,改善学习与记忆功能。
中图分类号:
关莹,于国强,唐祎周,辛贵乐,李季,王璐,张立,张春艳. 头穴艾灸联合跑台训练对血管性痴呆大鼠学习记忆和氧化应激的效果[J]. 《中国康复理论与实践》, 2022, 28(8): 927-933.
GUAN Ying,YU Guoqiang,TANG Yizhou,XIN Guile,LI Ji,WANG Lu,ZHANG Li,ZHANG Chunyan. Effects of scalp moxibustion combined with treadmill training on learning and memory, and oxidative stress in rats with vascular dementia[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(8): 927-933.
表3
各组氧化应激水平比较"
组别 | n | MDA/μmol·g-1 | SOD/U·g-1 | GSH/μmol·g-1 | GSH-Px/U·g-1 |
---|---|---|---|---|---|
假手术组 | 8 | 0.90±0.03 | 31.76±1.18 | 8.66±0.42 | 93.92±2.19 |
模型组 | 8 | 4.43±0.31a | 12.79±0.89a | 2.79±0.21a | 27.65±0.98a |
针康组 | 8 | 3.35±0.18a,b | 22.36±1.30a,b | 5.32±0.16a,b | 61.96±1.28a,b |
灸康组 | 8 | 2.42±0.19a,b,c | 28.95±1.82a,b,c | 7.26±0.19a,b,c | 77.90±1.92a,b,c |
F值 | 429.295 | 316.753 | 750.378 | 2319.014 | |
P值 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
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