线栓法建立卒中后吞咽障碍的大鼠模型

doi:10.3969/j.issn.1006-9771.2023.10.005

《中国康复理论与实践》 ›› 2023, Vol. 29 ›› Issue (10): 1147-1153.doi: 10.3969/j.issn.1006-9771.2023.10.005

线栓法建立卒中后吞咽障碍的大鼠模型

黄志霖¹, 徐发邵², 施静², 黄淦¹, 刘梅芳², 张霞辉²()

1.云南中医药大学，云南昆明市 650500
2.云南中医药大学第一附属医院/云南省中医医院，云南昆明市 650021

收稿日期:2022-10-08 修回日期:2023-07-11 出版日期:2023-10-25 发布日期:2023-11-16
通讯作者: 张霞辉(1982-)，男，硕士，主治医师，主要研究方向：卒中及相关并发症的针灸康复治疗。E-mail： 754258082@qq.com
作者简介:黄志霖(1997-)，男，汉族，广东汕尾市人，硕士研究生，主要研究方向：针灸防治脑病的基础及临床应用研究。
基金资助:
云南省科技厅云南中医药大学应用基础研究联合专项(202001AZ070001-049);国家自然科学基金项目(82060901)

Establishment of rat model of dysphagia after stroke by thread embolism

HUANG Zhilin¹, XU Fashao², SHI Jing², HUANG Gan¹, LIU Meifang², ZHANG Xiahui²()

1. Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, China
2. The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine/Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming, Yunnan 650021, China

Received:2022-10-08 Revised:2023-07-11 Published:2023-10-25 Online:2023-11-16
Contact: ZHANG Xiahui, E-mail: 754258082@qq.com
Supported by:
Yunnan Provincial Department of Science and Technology-Yunnan University of Traditional Chinese Medicine Applied Basic Research Joint Project(202001AZ070001-049);National Natural Science Foundation of China(82060901)

摘要/Abstract

摘要：

目的建立一种可行的卒中后吞咽障碍动物模型。
方法清洁级Sprague-Dawley大鼠22只随机分为正常组(n = 11)和造模组(n = 11)。造模组采用线栓法制备卒中后吞咽障碍动物模型，正常组不予处理。分别于造模成功3 d、7 d后记录所有大鼠第1次吞咽的发作潜伏期和吞咽次数，采用TTC染色法检测脑组织梗死情况，采用TUNEL荧光染色法测定缺血脑组织神经细胞凋亡情况。
结果与正常组比较，造模组造模3 d后吞咽潜伏期有所延长，吞咽次数减少，但均无显著性差异(P > 0.05);7 d后吞咽潜伏期延长(P < 0.05)，吞咽次数有所减少，但无显著性差异(P > 0.05)。与正常组比较，造模组脑组织出现明显梗死灶和大量凋亡细胞，体质量减小(P < 0.05)。
结论造模大鼠出现一定程度的吞咽障碍症状，有望成为脑卒中后吞咽障碍的转化模型。

关键词: 脑卒中, 吞咽障碍, 动物模型, 大鼠

Abstract:

Objective To explore a feasible animal model of dysphagia after stroke.
Methods Twenty-two clean Sprague-Dawley rats were randomly divided into normal group (n = 11) and model group (n = 11). The model of dysphagia after stroke was established by the thread embolism, and the normal group received no intervention. The latency of the first swallowing attack and the number of swallowing were recorded three and seven days after modeling. The cerebral infarction was detected by TTC staining, and the neuronal apoptosis in ischemic brain was detected by TUNEL fluorescence staining.
Results Compared with the normal group, the swallowing latency prolonged and the number of swallowing reduced three days in the model group, however, there was no significant difference (P > 0.05); seven days after modeling, the swallowing latency prolonged (P < 0.05), and the number of swallowing slightly reduced with little significant difference (P > 0.05). Compared with the normal group, the brain tissue showed obvious infarction area and a large number of apoptotic cells, while the body mass reduced in the model group (P < 0.05).
Conclusion The model rats express some features of dysphagia, which may become a transformation model of dysphagia after stroke.

Key words: stroke, dysphagia, animal model, rats

中图分类号:

R743.3

黄志霖, 徐发邵, 施静, 黄淦, 刘梅芳, 张霞辉. 线栓法建立卒中后吞咽障碍的大鼠模型[J]. 《中国康复理论与实践》, 2023, 29(10): 1147-1153.

HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.

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组别	n	吞咽潜伏期
正常组	8	5.35(3.73, 5.58)
造模组3 d后	7	17.00(5.00, 30.00)
造模组7 d后	7	32.00(7.00, 34.00)^a

组别	n	吞咽次数
正常组	11	14.00(7.25, 40.00)
造模组3 d后	10	4.50(1.75, 35.75)
造模组7 d后	7	12.00(3.00, 12.00)

组别	n	造模前	造模后第1天	造模后第2天	造模后第3天	造模后第4天	造模后第5天	造模后第6天	造模后第7天
正常组	11	196.15±5.85	204.26±9.93^a	220.53±14.18^a	227.33±13.11^a	226.86±11.50^a	246.90±12.76^a	251.31±15.57^a	264.81±13.26^a
造模组	7	198.83±10.92	181.79±13.48^a,b	172.81±20.99^a,b	169.22±28.39^a,b	168.00±33.96^a,b	176.08±35.77^a,b	179.22±35.87^b	191.09±39.77^b

线栓法建立卒中后吞咽障碍的大鼠模型

Establishment of rat model of dysphagia after stroke by thread embolism

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