丰富环境训练结合头穴丛刺对孤独症谱系障碍模型大鼠行为的影响

doi:10.3969/j.issn.1006-9771.2024.02.006

《中国康复理论与实践》 ›› 2024, Vol. 30 ›› Issue (2): 176-182.doi: 10.3969/j.issn.1006-9771.2024.02.006

丰富环境训练结合头穴丛刺对孤独症谱系障碍模型大鼠行为的影响

穆姿辰¹, 唐强², 史云秋¹, 王艳², 朱淑伟², 庄亚楠², 徐丹双², 李宏玉², 李保龙¹^,², 张春艳¹^,²(), 袁孟珂¹

1.黑龙江中医药大学，黑龙江哈尔滨市 150040
2.黑龙江中医药大学附属第二医院，黑龙江哈尔滨市 150001

收稿日期:2023-03-06 修回日期:2024-01-10 出版日期:2024-02-25 发布日期:2024-03-01
通讯作者: 张春艳 E-mail:676477397@qq.com
作者简介:穆姿辰(1996-)，女，汉族，黑龙江哈尔滨市人，博士研究生，主要研究方向：神经系统疾病中医康复及临床研究。
基金资助:
黑龙江省自然科学基金项目(LH2020H079)

Effect of enriched environment combined with acupuncture at head points on behavior in rats with autism spectrum disorder

MU Zichen¹, TANG Qiang², SHI Yunqiu¹, WANG Yan², ZHU Shuwei², ZHUANG Ya'nan², XU Danshuang², LI Hongyu², LI Baolong¹^,², ZHANG Chunyan¹^,²(), YUAN Mengke¹

1. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China
2. The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China

Received:2023-03-06 Revised:2024-01-10 Published:2024-02-25 Online:2024-03-01
Contact: ZHANG Chunyan E-mail:676477397@qq.com
Supported by:
Natural Science Foundation of Heilongjiang(LH2020H079)

摘要/Abstract

摘要：

目的观察丰富环境训练结合头穴丛刺对孤独症大鼠行为的影响。
方法选择健康雌性Wistar大鼠，于妊娠第12.5天时给予腹腔注射丙戊酸钠，随机选取子代雄鼠24只，随机分为模型组(n = 6)、丰富环境组(n = 6)、头穴丛刺组(n = 6)和丰富环境结合头穴丛刺组(n = 6)。对照组为腹腔注射等量生理盐水的雌鼠所产下的子代雄鼠(n = 6)。子代雄鼠21日龄时分别进行干预，治疗4周后对各组进行三箱实验、埋珠实验，计算社交能力指数、社交偏好指数和埋珠数量;采用酶联免疫吸附法(ELISA法)测定各组外周血白细胞介素(IL)-1β和IL-6的水平。
结果干预后，与模型组比较，丰富环境组、头穴丛刺组和丰富环境结合头穴丛刺组社交能力指数和社交偏好指数均改善(P < 0.05)，重复刻板行为均减少(P < 0.05)，外周血液中IL-6、IL-1β水平均下降(P < 0.05)，其中丰富环境结合头穴丛刺组最佳(P < 0.01)。
结论丰富环境训练和头穴丛刺均能够改善孤独症大鼠行为学症状，降低炎性因子表达，联合应用效果最佳。

关键词: 孤独症谱系障碍, 头穴丛刺, 丰富环境, 行为, 大鼠

Abstract:

Objective To observe the effect of enriched environment (EE) combined with acupuncture at head point (HA) on behavior in rats with autism spectrum disorder.
Methods Healthy female Wistar rats were given peritoneal injection of sodium valproate at 12.5 days of gestation. Twenty-four male offspring rats were randomly selected and then randomly divided into model group (n = 6), EE group (n = 6), HA group (n = 6) and EE combined with HA group (the combined group, n = 6). Six male offspring rats born from female mice injected with the same amount of saline intraperitoneally were as control group. After four weeks of treatment, all the five groups were tested with three-chamber test and marble burying test, and the sociability index, the social novelty index and the number of buried marbles were recorded. The levels of interleukin (IL)-1β and IL-6 in peripheral blood were determined by enzyme-linked immunosorbent assay (ELISA).
Results After treatment, compared with the model group, the sociability index and the social novelty index improved (P < 0.05), the number of buried marbles reduced (P < 0.05), and the levels of IL-6 and IL-1β in peripheral blood decreased in EE group, HA group and the combined group (P < 0.05); while the combined group was the best (P < 0.01).
Conclusion Both EE or acupuncture at HA could improve behavioral symptoms, and reduce the expression of inflammatory factors in rats with autism spectrum disorder. The combination of the two methods showed the best result.

Key words: autism spectrum disorder, acupuncture at head point, enriched environment, behavior, rats

中图分类号:

R749.94

穆姿辰, 唐强, 史云秋, 王艳, 朱淑伟, 庄亚楠, 徐丹双, 李宏玉, 李保龙, 张春艳, 袁孟珂. 丰富环境训练结合头穴丛刺对孤独症谱系障碍模型大鼠行为的影响[J]. 《中国康复理论与实践》, 2024, 30(2): 176-182.

MU Zichen, TANG Qiang, SHI Yunqiu, WANG Yan, ZHU Shuwei, ZHUANG Ya'nan, XU Danshuang, LI Hongyu, LI Baolong, ZHANG Chunyan, YUAN Mengke. Effect of enriched environment combined with acupuncture at head points on behavior in rats with autism spectrum disorder[J]. Chinese Journal of Rehabilitation Theory and Practice, 2024, 30(2): 176-182.

图/表 5

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组别	n	社交能力指数	社交偏好指数
对照组	6	4.20±0.13	2.15±0.11
模型组	6	0.71±0.07^a	0.08±0.05^a
丰富环境组	6	2.10±0.10^b	1.47±0.09^b
头穴丛刺组	6	2.04±0.14^b	1.52±0.09^b
丰富环境结合头穴丛刺组	6	2.83±0.15^b,c,d	1.85±0.07^b,c,d
F值		668.302	218.728
P值		< 0.001	< 0.001

组别	n	埋珠数量
对照组	6	4.16±1.17
模型组	6	16.50±1.38^a
丰富环境组	6	12.33±0.82^b
头穴丛刺组	6	13.33±1.03^b
丰富环境结合头穴丛刺组	6	8.83±4.40^b,c,d
F值		119.716
P值		< 0.001

组别	n	IL-6	IL-1β
对照组	6	11.68±0.80	16.11±0.49
模型组	6	21.29±1.04^a	24.72±0.56^a
丰富环境组	6	16.34±1.04^b	19.98±0.75^b
头穴丛刺组	6	16.91±1.14^b	20.59±0.75^b
丰富环境结合头穴丛刺组	6	13.97±3.39^b,c,d	17.95±0.91^b,c,d
F值		84.442	126.071
P值		< 0.001	< 0.001

丰富环境训练结合头穴丛刺对孤独症谱系障碍模型大鼠行为的影响

Effect of enriched environment combined with acupuncture at head points on behavior in rats with autism spectrum disorder

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