《中国康复理论与实践》

《中国康复理论与实践》 ›› 2001, Vol. 7 ›› Issue (03): 120-121.

• 论著 • 上一篇    下一篇

蚯蚓组织中硫氧还蛋白还原酶抑制剂初探

崔泓1,2; 贾慧敏1; 张祖珣1; 钟良纬3   

  1. 1.首都医科大学 北京市 100054;2.中国康复研究中心基础医学研究所 北京市 100077;3.Medical Nobel Institute for Biochemistry Department of Medical Biochemistry and Biophysics Karolinska Institute,SE-171 77Stockholm;Sweden
  • 收稿日期:2001-03-02 出版日期:2001-09-25 发布日期:2001-09-25

Initial study on the inhibitor of thioredoxin reductase in earthworm

CUI Hong, JIA Hui-min, ZHANG Zu-xun, et al   

  1. Institute of Basic Rehabilitation Medicine,China Rehabilitation Research Center, Beijing100077, China
  • Received:2001-03-02 Published:2001-09-25 Online:2001-09-25

PDF (PC)

摘要： 目的研究蚯蚓组织成份对硫氧还蛋白系统的影响。方法乙酸盐缓冲液抽提蚯蚓组织成份,不同处理的蚯蚓提取液与硫氧还蛋白系统反应。在340nm波长处,用NADPH作为被监测的反应底物来动态监测硫氧还蛋白还原酶(TrxR)的催化活性。结果蚯蚓提取液与TrxR单独作用后,监测底物NADPH的吸光值几乎不发生变化。蚯蚓提取液的溶剂及煮沸后的上清液不具有以上作用。结论蚯蚓组织中含有能够抑制TrxR活性的物质,该物质的化学本质为蛋白质。

关键词: 蚯蚓, 硫氧还蛋白, 硫氧还蛋白还原酶, NADPH, 硫氧还蛋白还原酶抑制剂

Abstract: Objective To study the effect of the tissue components purified from earthworm on the thioredoxin system and its pharmcological mechanism. MethodsAcetate buffer purified the tissue components from the earthworm. The catalyzing activity of the thioredoxin reductases was dynamically measured on the 340nm wavelengthby the NADPH, an indicator by means of the reaction between the tissue components and thioredoxin system. ResultsThe absorbency of the NADPH was almost unchanged in the process of the reaction between the purified tissue components of earthworm and the thioredoxin reductase. On the contrary, the solvent to purify earthworm liquid and the clear liquid from boiled purified earthworm liquid had not above mentioned function. ConclusionsThere are some kinds of proteins in the tissue components of the earthworm, which could inhibit the activity of the thioredoxin reductase.

Key words: earthworm, thioredoxin, thioredoxin reductase, NADPH, thioredoxin reductase inhibitor