《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (01): 13-15.

• 基础研究 • 上一篇    下一篇

中药复方治疗大鼠缺血性脑损伤的药理研究

许健鹏1,3; 董浩2; 陈之罡1; 杨健2   

  1. 1.北京博爱医院中医科 北京市 100077;2.中国康复研究中心康复医学研究所 北京市 100077;3.首都医科大学康复医学院 北京市 100077
  • 收稿日期:2001-08-20 出版日期:2002-01-25 发布日期:2002-01-25

Effect of complex prescription of Chinese medicine on ischemic brain of rat

XU Jian-peng, DONG Hao, CHEN Zhi-gang, et al   

  1. Faculty of Rehabilitation, Capital University of Medical Scienes, Beijing 100077, China
  • Received:2001-08-20 Published:2002-01-25 Online:2002-01-25

PDF (PC)

被引次数

15

摘要： 目的研究中药复方在缺血性脑损伤时对脑组织的保护作用。方法 75只Wistar大鼠随机分为5组。第1组为伪手术组,其余4组剥离颈动脉后结扎,并切断颈总动脉。第2组为造模组。术后第20h起,第3、4、5组分别灌喂中药复方水煎液Ⅰ、Ⅱ、Ⅲ号方各1次。给药3h后,第2—5组分别置于低氧环境1h,以后连续给药7天,每天1次。术后第7天从右心房取血,处死动物,取全部右脑,分别测定脑组织和血清中的MDA、NOS、SOD及脑组织钙的含量,并观察海马CA1区锥体细胞的排列情况。结果Ⅰ、Ⅱ、Ⅲ号方均可降低脑组织和血清MDA的含量 ,并能降低脑组织钙的含量(P<0.05—0.001)。Ⅰ号方还可增加脑组织SOD的活性,Ⅱ、Ⅲ号方可降低脑组织NOS的活性。第3、4、5组海马CA1区锥体细胞排列较整齐,坏死数目较造模组减少,其中第4、5组更为明显。结论中药Ⅰ、Ⅱ、Ⅲ号方对缺血性大鼠的脑组织有保护作用。

关键词: 中药, 方剂, 缺血性脑损伤, 丙二醛(MDA), 一氧化氮合成酶(NOS), 超氧化物歧化酶(SOD), 钙, 动物实验

Abstract: ObjectiveTo study the protection of Chinese herbs on the ischemic brain of rats. Methods 75 Wistar rats were divided into 5 groups, Group 1 for false operation. For the other four groups, the common carotid artery was exposed then was ligatured and cut off, Group 2 for model. From the 20th hour after operation on,Group 3, 4, 5 were fed with complex prescription of Chinese medicine named Prescription 1, 2 and 3 once a day respectively. 3 hours after taking medicine, Groups 2-5 were put in the hypoxia environment for 1 hours, then taking the medicine for 7 days. On the 7th day after operation,the blood was taken from R. atria then the rats were killed and the whole right brains were cut off. Malonaldehyde (MDA), notric oxide synthetase (NOS) and superoxide dismutase (SOD) in the brain tissue and serum, and calcium in the brain were measured respectively. ResultsThe three prescriptions can decrease the quantity of MDA both in brain tissue and serum and the calcium in brain tissue(P<0.05-0.001).Prescription 1 can enhance the activity of SOD in brain tissue while the others can decrease the activity of NOS. The hippocampus cells show tidy, and the number of the necrotic cells decrease greatly among them, Groups 4 and 5 are better than Group 3.Conclusions Prescriptions 1-3 can protect the brain tissue of the rat form ischemic brain injury.

Key words: Chinese herbs, complex prescription, ischemic brain injury, malonaldehyde (MDA), notric oxide synthetase (NOS), superoxide dismutase (SOD), calcium, experiment