《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (01): 5-7.

• 专题研究 • 上一篇    下一篇

短波紫外线照射对创伤局部转化生长因子β表达的影响

索伟1; 王兴林1; 王德文2   

  1. 1.解放军总医院理疗科 北京市 100853;2.军事医学科学院放射医学研究所 北京市 100853
  • 收稿日期:2001-08-06 出版日期:2002-01-25 发布日期:2002-01-25

Effect of ultraviolet C irradiation on expression of transforming growth factor β in wound

SUO Wei,WANG Xing-lin, WANG De-wen   

  1. Department of Physiotherapy, The General Hospital of PLA, Beijing 100853, China
  • Received:2001-08-06 Published:2002-01-25 Online:2002-01-25

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摘要： 目的研究不同剂量短波紫外线(UVC)照射对伤口肉芽组织中转化生长因子β(TGF-β)表达的影响。方法UVC 15mJ/cm2,15s/d和60mJ/cm2,60s/d分别照射大鼠背部伤口3d,采用免疫组化和原位杂交的方法观察伤口肉芽组织中TGF-β的表达。结果致伤后照射7天时,15mJ/cm2 照射组TGF-β在mRNA水平与蛋白水平的表达均高于60mJ/cm2照射组和对照组(P<0.05);照射21天时,60mJ/cm2 照射组TGF-βmRNA表达高于15mJ/cm2照射组和对照组(P<0 .05)。结论在伤口愈合早期15mJ/cm2UVC照射能促进TGF-β的表达,有利于组织修复;愈合晚期,60mJ/cm2照射使TGF-β的表达有逐渐增高的趋势。

关键词: 短波紫外线, 肉芽组织, 转化生长因子β

Abstract: ObjectiveTo study the effect of ultraviolet C(UVC) irradiation at different doses on expression of transforming growth factor β(TGF-β) of granulation tissues in wound. MethodsAfter dosing 15mJ/cm2 or 60mJ/cm2 UVC on wound of rats, the expression of TGF-β were observed at both the mRNA level and the protein level by the methods of in situ hybridization and immunohistochemistry respectively. Results At the 7th day after UVC irradiation, the expression of TGF-β in the 15mJ/cm2 group were higher than that in 60mJ/cm2 group and controls (P<0.05)at both the mRNA and the protein level. On the 21st day, the level of TGF-β mRNA in the 60mJ/cm2 group was higher than that in the other two groups(P<0.05).Conclusions At the early stage of wound healing,the treatment of 15mJ/cm2 UVC irradiation promots the expression of TGF-β and might be useful for accelerating wound healing. The level of TGF-β mRNA was up-regulated at the later stage at the dose of 60mJ/cm2 UVC irradiation.

Key words: ultraviolet C(UVC), granulation tissue, transforming growth factor β(TGF-β)