《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (05): 296-297.

• 专题研究 • 上一篇    下一篇

移植用微囊化神经细胞/组织制备及运输的实验研究

雄鹰1; 于炜婷1; 王为1; 马小军1; 陈绍宗2   

  1. 1.中国科学院大连化学物理研究所生物技术部生物医用材料工程组 大连市 116023;2.第四军用大学塘都医院整形外科 西安市 710038
  • 收稿日期:2002-03-28 出版日期:2002-05-25 发布日期:2002-05-25

Experimental study of microencapsulated nerve cell/tissue for transplantation

XIONG Ying,YU Wei-ting,WANG Wei,et al   

  1. Biochemical Engineering Departmant,DaLian Institute of Chemical Physics. DaLian 116023,Shandong,China
  • Received:2002-03-28 Published:2002-05-25 Online:2002-05-25

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被引次数

69

摘要： 目的探讨海藻酸钠/聚赖氨酸(APA)微胶囊用于神经细胞/组织移植的可行性。方法使用大功率高压脉冲微胶囊制备仪制备APA微胶囊,包埋分离自大鼠周围神经的神经细胞/组织;应用ELISA双抗体夹心法检测微囊化神经细胞/组织培养液中神经生长因子(NGF)的浓度。结果包埋神经细胞/组织的APA微胶囊在培养后及经过一定时间的空运后,囊内的神经细胞 /组织正常生存并保持分泌神经生长因子的功能。结论微囊化的神经细胞/组织可以在体外培养和运输,并可分泌NGF。

关键词: 微胶囊, 神经生长因子, 细胞移植

Abstract: ObjectiveTo investigate the feasibility of poly l lysne to preparation microencapsules for cell transplantation therapies.MethodsUsing drop generative technique preparation Alginate poly l lysne Alginate (APA) microencapsules containing nerve cell/tissue. The concentration of nerve growth factor in supernatant was detected by two antibody sandwich method of enzyme linked immunosorbent assay.ResultsThe nerve cell/tissue in microencapsules retain reliable cell viability and function. Conclusions The APA is proved with reliable biocompatibility and strength,would work as an immunoisolation tools to exert important function in nerve renovate.

Key words: microencapsules, nerve growth factor, cell transplantation